Gordonia phage Beyoncage

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Terapinvirus; unclassified Terapinvirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MBC2|A0A345MBC2_9CAUD Ribbon-helix-helix DNA binding domain protein OS=Gordonia phage Beyoncage OX=2283245 GN=82 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84SRR4 pKa = 11.84SRR6 pKa = 11.84KK7 pKa = 9.26CDD9 pKa = 3.35YY10 pKa = 10.95CGTNLASEE18 pKa = 4.48FDD20 pKa = 3.67EE21 pKa = 4.73MNDD24 pKa = 3.36GTRR27 pKa = 11.84MCTEE31 pKa = 5.09CYY33 pKa = 10.05DD34 pKa = 3.75EE35 pKa = 5.17QYY37 pKa = 11.75GEE39 pKa = 4.24GDD41 pKa = 3.36YY42 pKa = 11.3SGTGQTVDD50 pKa = 3.25PEE52 pKa = 4.31EE53 pKa = 4.37FNEE56 pKa = 4.16GDD58 pKa = 3.18NRR60 pKa = 11.84TEE62 pKa = 4.14FNVLSPDD69 pKa = 3.31EE70 pKa = 4.28VASSNGG76 pKa = 3.23

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MB46|A0A345MB46_9CAUD Uncharacterized protein OS=Gordonia phage Beyoncage OX=2283245 GN=6 PE=4 SV=1
MM1 pKa = 7.42GKK3 pKa = 9.35RR4 pKa = 11.84WGNALGGWKK13 pKa = 9.13YY14 pKa = 9.83QKK16 pKa = 8.97RR17 pKa = 11.84TKK19 pKa = 10.4KK20 pKa = 9.66GTFAGLGLRR29 pKa = 11.84GKK31 pKa = 7.15TRR33 pKa = 11.84KK34 pKa = 9.21GVKK37 pKa = 9.1KK38 pKa = 10.72VKK40 pKa = 10.19GSLRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.59RR47 pKa = 11.84RR48 pKa = 3.24

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

21164

37

2256

220.5

24.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.233 ± 0.536

0.775 ± 0.112

6.667 ± 0.34

7.466 ± 0.269

3.426 ± 0.134

8.604 ± 0.439

2.018 ± 0.172

4.89 ± 0.178

5.599 ± 0.273

7.305 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.118

3.667 ± 0.129

4.805 ± 0.223

4.271 ± 0.19

6.261 ± 0.248

5.49 ± 0.193

5.382 ± 0.211

6.724 ± 0.159

1.862 ± 0.162

2.939 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski