Amino acid dipepetide frequency for Mycobacterium phage Harley

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.023AlaAla: 15.023 ± 1.673
1.18AlaCys: 1.18 ± 0.279
7.243AlaAsp: 7.243 ± 0.664
7.994AlaGlu: 7.994 ± 0.845
2.683AlaPhe: 2.683 ± 0.348
10.248AlaGly: 10.248 ± 1.186
2.468AlaHis: 2.468 ± 0.443
4.024AlaIle: 4.024 ± 0.519
4.239AlaLys: 4.239 ± 0.391
8.585AlaLeu: 8.585 ± 0.775
2.79AlaMet: 2.79 ± 0.361
2.79AlaAsn: 2.79 ± 0.402
4.453AlaPro: 4.453 ± 0.488
3.541AlaGln: 3.541 ± 0.384
7.136AlaArg: 7.136 ± 0.706
5.258AlaSer: 5.258 ± 0.63
6.975AlaThr: 6.975 ± 0.666
6.546AlaVal: 6.546 ± 0.645
3.112AlaTrp: 3.112 ± 0.608
2.2AlaTyr: 2.2 ± 0.334
0.0AlaXaa: 0.0 ± 0.0
Cys
1.019CysAla: 1.019 ± 0.231
0.161CysCys: 0.161 ± 0.089
1.288CysAsp: 1.288 ± 0.329
0.858CysGlu: 0.858 ± 0.222
0.161CysPhe: 0.161 ± 0.101
1.824CysGly: 1.824 ± 0.446
0.268CysHis: 0.268 ± 0.139
0.161CysIle: 0.161 ± 0.114
0.376CysLys: 0.376 ± 0.152
0.912CysLeu: 0.912 ± 0.246
0.107CysMet: 0.107 ± 0.077
0.322CysAsn: 0.322 ± 0.13
1.019CysPro: 1.019 ± 0.228
0.322CysGln: 0.322 ± 0.143
0.966CysArg: 0.966 ± 0.306
0.912CysSer: 0.912 ± 0.328
0.59CysThr: 0.59 ± 0.192
0.537CysVal: 0.537 ± 0.152
0.322CysTrp: 0.322 ± 0.121
0.161CysTyr: 0.161 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
6.76AspAla: 6.76 ± 0.699
0.966AspCys: 0.966 ± 0.204
4.561AspAsp: 4.561 ± 0.55
3.97AspGlu: 3.97 ± 0.505
1.985AspPhe: 1.985 ± 0.293
6.76AspGly: 6.76 ± 0.669
1.449AspHis: 1.449 ± 0.286
2.307AspIle: 2.307 ± 0.31
1.824AspLys: 1.824 ± 0.287
5.741AspLeu: 5.741 ± 0.558
1.288AspMet: 1.288 ± 0.306
1.556AspAsn: 1.556 ± 0.365
5.043AspPro: 5.043 ± 0.535
2.468AspGln: 2.468 ± 0.33
4.99AspArg: 4.99 ± 0.512
3.809AspSer: 3.809 ± 0.482
4.453AspThr: 4.453 ± 0.575
4.346AspVal: 4.346 ± 0.499
1.341AspTrp: 1.341 ± 0.281
1.985AspTyr: 1.985 ± 0.338
0.0AspXaa: 0.0 ± 0.0
Glu
6.814GluAla: 6.814 ± 0.906
1.127GluCys: 1.127 ± 0.241
2.897GluAsp: 2.897 ± 0.429
2.79GluGlu: 2.79 ± 0.509
2.039GluPhe: 2.039 ± 0.319
3.219GluGly: 3.219 ± 0.407
1.717GluHis: 1.717 ± 0.37
2.629GluIle: 2.629 ± 0.347
1.771GluLys: 1.771 ± 0.293
5.312GluLeu: 5.312 ± 0.624
1.341GluMet: 1.341 ± 0.283
2.039GluAsn: 2.039 ± 0.319
3.434GluPro: 3.434 ± 0.506
2.79GluGln: 2.79 ± 0.471
4.614GluArg: 4.614 ± 0.606
3.541GluSer: 3.541 ± 0.643
4.185GluThr: 4.185 ± 0.504
3.38GluVal: 3.38 ± 0.457
1.717GluTrp: 1.717 ± 0.301
1.717GluTyr: 1.717 ± 0.319
0.0GluXaa: 0.0 ± 0.0
Phe
2.683PheAla: 2.683 ± 0.329
0.268PheCys: 0.268 ± 0.132
2.522PheAsp: 2.522 ± 0.454
1.878PheGlu: 1.878 ± 0.326
1.18PhePhe: 1.18 ± 0.285
2.844PheGly: 2.844 ± 0.542
0.322PheHis: 0.322 ± 0.123
1.556PheIle: 1.556 ± 0.322
1.18PheLys: 1.18 ± 0.263
1.878PheLeu: 1.878 ± 0.296
0.644PheMet: 0.644 ± 0.187
1.341PheAsn: 1.341 ± 0.314
1.717PhePro: 1.717 ± 0.318
1.127PheGln: 1.127 ± 0.284
1.61PheArg: 1.61 ± 0.309
1.502PheSer: 1.502 ± 0.26
2.468PheThr: 2.468 ± 0.404
1.932PheVal: 1.932 ± 0.238
0.537PheTrp: 0.537 ± 0.17
0.858PheTyr: 0.858 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
8.96GlyAla: 8.96 ± 1.104
1.288GlyCys: 1.288 ± 0.295
6.224GlyAsp: 6.224 ± 0.492
3.917GlyGlu: 3.917 ± 0.497
3.005GlyPhe: 3.005 ± 0.464
11.321GlyGly: 11.321 ± 1.947
1.771GlyHis: 1.771 ± 0.281
4.239GlyIle: 4.239 ± 0.535
2.575GlyLys: 2.575 ± 0.428
5.365GlyLeu: 5.365 ± 0.624
2.307GlyMet: 2.307 ± 0.431
2.683GlyAsn: 2.683 ± 0.401
3.917GlyPro: 3.917 ± 0.662
2.468GlyGln: 2.468 ± 0.592
5.097GlyArg: 5.097 ± 0.629
6.277GlySer: 6.277 ± 0.776
6.277GlyThr: 6.277 ± 0.69
6.546GlyVal: 6.546 ± 0.607
2.575GlyTrp: 2.575 ± 0.392
1.824GlyTyr: 1.824 ± 0.343
0.0GlyXaa: 0.0 ± 0.0
His
2.146HisAla: 2.146 ± 0.386
0.429HisCys: 0.429 ± 0.176
0.858HisAsp: 0.858 ± 0.227
1.073HisGlu: 1.073 ± 0.268
0.537HisPhe: 0.537 ± 0.176
1.663HisGly: 1.663 ± 0.287
1.019HisHis: 1.019 ± 0.25
1.18HisIle: 1.18 ± 0.247
0.697HisLys: 0.697 ± 0.221
1.663HisLeu: 1.663 ± 0.307
0.483HisMet: 0.483 ± 0.14
0.697HisAsn: 0.697 ± 0.198
1.771HisPro: 1.771 ± 0.299
0.966HisGln: 0.966 ± 0.299
1.932HisArg: 1.932 ± 0.386
1.019HisSer: 1.019 ± 0.217
1.449HisThr: 1.449 ± 0.33
1.663HisVal: 1.663 ± 0.269
0.644HisTrp: 0.644 ± 0.174
0.912HisTyr: 0.912 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
6.224IleAla: 6.224 ± 0.56
0.537IleCys: 0.537 ± 0.216
3.756IleAsp: 3.756 ± 0.473
3.058IleGlu: 3.058 ± 0.358
0.912IlePhe: 0.912 ± 0.258
4.078IleGly: 4.078 ± 0.402
1.556IleHis: 1.556 ± 0.381
1.449IleIle: 1.449 ± 0.328
0.966IleLys: 0.966 ± 0.235
2.039IleLeu: 2.039 ± 0.324
0.429IleMet: 0.429 ± 0.146
1.771IleAsn: 1.771 ± 0.276
2.468IlePro: 2.468 ± 0.314
1.395IleGln: 1.395 ± 0.253
2.629IleArg: 2.629 ± 0.399
2.307IleSer: 2.307 ± 0.419
3.327IleThr: 3.327 ± 0.439
3.166IleVal: 3.166 ± 0.346
0.858IleTrp: 0.858 ± 0.244
0.858IleTyr: 0.858 ± 0.187
0.0IleXaa: 0.0 ± 0.0
Lys
3.702LysAla: 3.702 ± 0.477
0.537LysCys: 0.537 ± 0.189
1.341LysAsp: 1.341 ± 0.265
1.878LysGlu: 1.878 ± 0.303
1.18LysPhe: 1.18 ± 0.249
2.844LysGly: 2.844 ± 0.397
1.18LysHis: 1.18 ± 0.313
0.966LysIle: 0.966 ± 0.229
1.502LysLys: 1.502 ± 0.4
2.468LysLeu: 2.468 ± 0.411
0.644LysMet: 0.644 ± 0.172
0.751LysAsn: 0.751 ± 0.206
2.146LysPro: 2.146 ± 0.4
1.18LysGln: 1.18 ± 0.205
2.575LysArg: 2.575 ± 0.425
2.092LysSer: 2.092 ± 0.316
2.253LysThr: 2.253 ± 0.306
2.575LysVal: 2.575 ± 0.419
0.912LysTrp: 0.912 ± 0.284
0.912LysTyr: 0.912 ± 0.255
0.0LysXaa: 0.0 ± 0.0
Leu
7.994LeuAla: 7.994 ± 0.747
0.59LeuCys: 0.59 ± 0.192
5.151LeuAsp: 5.151 ± 0.532
3.595LeuGlu: 3.595 ± 0.39
2.2LeuPhe: 2.2 ± 0.293
5.043LeuGly: 5.043 ± 0.511
1.18LeuHis: 1.18 ± 0.285
3.112LeuIle: 3.112 ± 0.449
2.253LeuLys: 2.253 ± 0.368
4.936LeuLeu: 4.936 ± 0.478
1.61LeuMet: 1.61 ± 0.303
2.575LeuAsn: 2.575 ± 0.405
5.258LeuPro: 5.258 ± 0.663
2.629LeuGln: 2.629 ± 0.407
5.687LeuArg: 5.687 ± 0.687
4.561LeuSer: 4.561 ± 0.526
5.58LeuThr: 5.58 ± 0.533
4.99LeuVal: 4.99 ± 0.491
1.127LeuTrp: 1.127 ± 0.289
2.092LeuTyr: 2.092 ± 0.33
0.0LeuXaa: 0.0 ± 0.0
Met
2.146MetAla: 2.146 ± 0.286
0.107MetCys: 0.107 ± 0.071
1.18MetAsp: 1.18 ± 0.25
0.912MetGlu: 0.912 ± 0.181
0.697MetPhe: 0.697 ± 0.207
1.932MetGly: 1.932 ± 0.288
0.107MetHis: 0.107 ± 0.08
0.751MetIle: 0.751 ± 0.233
0.858MetLys: 0.858 ± 0.227
1.932MetLeu: 1.932 ± 0.296
0.537MetMet: 0.537 ± 0.213
0.858MetAsn: 0.858 ± 0.239
1.288MetPro: 1.288 ± 0.246
0.537MetGln: 0.537 ± 0.169
1.502MetArg: 1.502 ± 0.247
2.736MetSer: 2.736 ± 0.436
1.985MetThr: 1.985 ± 0.295
1.234MetVal: 1.234 ± 0.301
0.322MetTrp: 0.322 ± 0.12
0.268MetTyr: 0.268 ± 0.111
0.0MetXaa: 0.0 ± 0.0
Asn
3.38AsnAla: 3.38 ± 0.421
0.215AsnCys: 0.215 ± 0.103
1.771AsnAsp: 1.771 ± 0.249
1.771AsnGlu: 1.771 ± 0.312
0.805AsnPhe: 0.805 ± 0.268
3.809AsnGly: 3.809 ± 0.471
0.805AsnHis: 0.805 ± 0.211
1.878AsnIle: 1.878 ± 0.408
1.073AsnLys: 1.073 ± 0.268
1.824AsnLeu: 1.824 ± 0.319
0.429AsnMet: 0.429 ± 0.14
1.61AsnAsn: 1.61 ± 0.337
2.414AsnPro: 2.414 ± 0.335
0.966AsnGln: 0.966 ± 0.268
1.878AsnArg: 1.878 ± 0.359
1.61AsnSer: 1.61 ± 0.271
1.878AsnThr: 1.878 ± 0.324
2.307AsnVal: 2.307 ± 0.385
0.912AsnTrp: 0.912 ± 0.192
0.858AsnTyr: 0.858 ± 0.162
0.0AsnXaa: 0.0 ± 0.0
Pro
5.365ProAla: 5.365 ± 0.738
0.644ProCys: 0.644 ± 0.199
4.936ProAsp: 4.936 ± 0.545
4.507ProGlu: 4.507 ± 0.427
1.771ProPhe: 1.771 ± 0.352
6.17ProGly: 6.17 ± 0.691
1.234ProHis: 1.234 ± 0.26
2.414ProIle: 2.414 ± 0.396
2.146ProLys: 2.146 ± 0.329
4.185ProLeu: 4.185 ± 0.542
1.234ProMet: 1.234 ± 0.302
1.878ProAsn: 1.878 ± 0.314
3.809ProPro: 3.809 ± 0.552
1.878ProGln: 1.878 ± 0.326
2.951ProArg: 2.951 ± 0.451
3.273ProSer: 3.273 ± 0.435
3.166ProThr: 3.166 ± 0.38
4.078ProVal: 4.078 ± 0.49
1.18ProTrp: 1.18 ± 0.259
1.771ProTyr: 1.771 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
4.4GlnAla: 4.4 ± 0.528
0.376GlnCys: 0.376 ± 0.168
1.502GlnAsp: 1.502 ± 0.258
1.556GlnGlu: 1.556 ± 0.322
1.019GlnPhe: 1.019 ± 0.204
2.575GlnGly: 2.575 ± 0.462
0.697GlnHis: 0.697 ± 0.223
1.824GlnIle: 1.824 ± 0.343
1.395GlnLys: 1.395 ± 0.231
2.951GlnLeu: 2.951 ± 0.353
0.644GlnMet: 0.644 ± 0.169
0.912GlnAsn: 0.912 ± 0.271
2.146GlnPro: 2.146 ± 0.381
1.288GlnGln: 1.288 ± 0.304
2.629GlnArg: 2.629 ± 0.369
1.985GlnSer: 1.985 ± 0.361
1.878GlnThr: 1.878 ± 0.32
2.146GlnVal: 2.146 ± 0.331
0.805GlnTrp: 0.805 ± 0.215
1.019GlnTyr: 1.019 ± 0.273
0.0GlnXaa: 0.0 ± 0.0
Arg
5.956ArgAla: 5.956 ± 0.648
1.073ArgCys: 1.073 ± 0.29
4.829ArgAsp: 4.829 ± 0.581
5.204ArgGlu: 5.204 ± 0.692
2.361ArgPhe: 2.361 ± 0.389
4.239ArgGly: 4.239 ± 0.474
1.61ArgHis: 1.61 ± 0.338
4.024ArgIle: 4.024 ± 0.488
2.414ArgLys: 2.414 ± 0.347
4.668ArgLeu: 4.668 ± 0.653
2.361ArgMet: 2.361 ± 0.381
2.575ArgAsn: 2.575 ± 0.448
3.648ArgPro: 3.648 ± 0.453
1.878ArgGln: 1.878 ± 0.421
5.473ArgArg: 5.473 ± 0.838
3.595ArgSer: 3.595 ± 0.361
3.273ArgThr: 3.273 ± 0.473
5.634ArgVal: 5.634 ± 0.732
2.146ArgTrp: 2.146 ± 0.39
1.717ArgTyr: 1.717 ± 0.323
0.0ArgXaa: 0.0 ± 0.0
Ser
6.331SerAla: 6.331 ± 1.023
0.537SerCys: 0.537 ± 0.248
4.346SerAsp: 4.346 ± 0.491
2.951SerGlu: 2.951 ± 0.406
1.878SerPhe: 1.878 ± 0.385
6.277SerGly: 6.277 ± 0.963
1.234SerHis: 1.234 ± 0.247
2.683SerIle: 2.683 ± 0.387
2.092SerLys: 2.092 ± 0.364
3.809SerLeu: 3.809 ± 0.441
1.395SerMet: 1.395 ± 0.273
2.092SerAsn: 2.092 ± 0.374
3.38SerPro: 3.38 ± 0.373
1.824SerGln: 1.824 ± 0.291
3.756SerArg: 3.756 ± 0.471
3.541SerSer: 3.541 ± 0.616
3.38SerThr: 3.38 ± 0.445
4.722SerVal: 4.722 ± 0.534
1.234SerTrp: 1.234 ± 0.259
1.449SerTyr: 1.449 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
6.76ThrAla: 6.76 ± 0.615
0.429ThrCys: 0.429 ± 0.174
4.078ThrAsp: 4.078 ± 0.575
3.917ThrGlu: 3.917 ± 0.417
2.039ThrPhe: 2.039 ± 0.331
5.848ThrGly: 5.848 ± 0.696
1.663ThrHis: 1.663 ± 0.357
3.595ThrIle: 3.595 ± 0.457
2.092ThrLys: 2.092 ± 0.296
4.185ThrLeu: 4.185 ± 0.491
1.18ThrMet: 1.18 ± 0.255
2.253ThrAsn: 2.253 ± 0.355
4.614ThrPro: 4.614 ± 0.634
2.039ThrGln: 2.039 ± 0.362
4.775ThrArg: 4.775 ± 0.569
3.863ThrSer: 3.863 ± 0.474
4.882ThrThr: 4.882 ± 0.661
5.58ThrVal: 5.58 ± 0.611
1.234ThrTrp: 1.234 ± 0.247
1.61ThrTyr: 1.61 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
8.531ValAla: 8.531 ± 0.614
1.395ValCys: 1.395 ± 0.315
5.848ValAsp: 5.848 ± 0.46
4.561ValGlu: 4.561 ± 0.454
2.2ValPhe: 2.2 ± 0.341
4.936ValGly: 4.936 ± 0.572
1.341ValHis: 1.341 ± 0.281
2.683ValIle: 2.683 ± 0.403
2.468ValLys: 2.468 ± 0.335
5.419ValLeu: 5.419 ± 0.591
1.127ValMet: 1.127 ± 0.178
1.985ValAsn: 1.985 ± 0.339
3.756ValPro: 3.756 ± 0.422
2.79ValGln: 2.79 ± 0.353
3.863ValArg: 3.863 ± 0.486
4.507ValSer: 4.507 ± 0.485
5.043ValThr: 5.043 ± 0.602
6.438ValVal: 6.438 ± 0.718
1.556ValTrp: 1.556 ± 0.292
1.449ValTyr: 1.449 ± 0.281
0.0ValXaa: 0.0 ± 0.0
Trp
1.932TrpAla: 1.932 ± 0.276
0.107TrpCys: 0.107 ± 0.073
1.61TrpAsp: 1.61 ± 0.318
1.019TrpGlu: 1.019 ± 0.317
0.644TrpPhe: 0.644 ± 0.141
0.966TrpGly: 0.966 ± 0.239
0.805TrpHis: 0.805 ± 0.255
1.127TrpIle: 1.127 ± 0.248
0.805TrpLys: 0.805 ± 0.188
2.146TrpLeu: 2.146 ± 0.337
1.073TrpMet: 1.073 ± 0.289
0.697TrpAsn: 0.697 ± 0.243
1.18TrpPro: 1.18 ± 0.271
0.858TrpGln: 0.858 ± 0.246
2.522TrpArg: 2.522 ± 0.407
1.61TrpSer: 1.61 ± 0.5
1.663TrpThr: 1.663 ± 0.332
1.985TrpVal: 1.985 ± 0.441
0.912TrpTrp: 0.912 ± 0.189
0.483TrpTyr: 0.483 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.361TyrAla: 2.361 ± 0.413
0.322TyrCys: 0.322 ± 0.156
1.663TyrAsp: 1.663 ± 0.341
1.663TyrGlu: 1.663 ± 0.274
0.751TyrPhe: 0.751 ± 0.177
2.039TyrGly: 2.039 ± 0.353
0.376TyrHis: 0.376 ± 0.104
1.18TyrIle: 1.18 ± 0.216
0.912TyrLys: 0.912 ± 0.224
2.146TyrLeu: 2.146 ± 0.331
0.215TyrMet: 0.215 ± 0.104
0.805TyrAsn: 0.805 ± 0.206
1.127TyrPro: 1.127 ± 0.21
0.805TyrGln: 0.805 ± 0.208
2.092TyrArg: 2.092 ± 0.34
0.966TyrSer: 0.966 ± 0.249
1.932TyrThr: 1.932 ± 0.375
2.092TyrVal: 2.092 ± 0.359
0.697TyrTrp: 0.697 ± 0.214
0.644TyrTyr: 0.644 ± 0.152
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (18639 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski