Amino acid dipepetide frequency for Streptococcus phage SW19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.401AlaAla: 5.401 ± 2.763
0.279AlaCys: 0.279 ± 0.15
3.445AlaAsp: 3.445 ± 0.616
3.352AlaGlu: 3.352 ± 0.425
2.514AlaPhe: 2.514 ± 0.809
4.47AlaGly: 4.47 ± 1.194
0.931AlaHis: 0.931 ± 0.284
7.263AlaIle: 7.263 ± 1.6
6.518AlaLys: 6.518 ± 0.859
5.68AlaLeu: 5.68 ± 1.102
3.073AlaMet: 3.073 ± 0.987
4.377AlaAsn: 4.377 ± 0.764
2.7AlaPro: 2.7 ± 0.504
2.7AlaGln: 2.7 ± 0.763
2.7AlaArg: 2.7 ± 0.429
4.004AlaSer: 4.004 ± 0.96
5.68AlaThr: 5.68 ± 1.282
4.377AlaVal: 4.377 ± 1.448
0.559AlaTrp: 0.559 ± 0.229
2.142AlaTyr: 2.142 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
0.279CysAla: 0.279 ± 0.164
0.186CysCys: 0.186 ± 0.189
0.652CysAsp: 0.652 ± 0.263
0.466CysGlu: 0.466 ± 0.212
0.0CysPhe: 0.0 ± 0.0
0.372CysGly: 0.372 ± 0.196
0.279CysHis: 0.279 ± 0.166
0.466CysIle: 0.466 ± 0.153
0.559CysLys: 0.559 ± 0.245
0.652CysLeu: 0.652 ± 0.295
0.0CysMet: 0.0 ± 0.0
0.093CysAsn: 0.093 ± 0.091
0.186CysPro: 0.186 ± 0.129
0.186CysGln: 0.186 ± 0.125
0.186CysArg: 0.186 ± 0.113
0.559CysSer: 0.559 ± 0.282
0.093CysThr: 0.093 ± 0.091
0.093CysVal: 0.093 ± 0.087
0.093CysTrp: 0.093 ± 0.099
0.372CysTyr: 0.372 ± 0.198
0.0CysXaa: 0.0 ± 0.0
Asp
2.887AspAla: 2.887 ± 0.588
0.279AspCys: 0.279 ± 0.166
5.494AspAsp: 5.494 ± 1.084
4.19AspGlu: 4.19 ± 0.631
3.911AspPhe: 3.911 ± 0.784
4.935AspGly: 4.935 ± 0.636
0.559AspHis: 0.559 ± 0.224
4.097AspIle: 4.097 ± 0.778
4.563AspLys: 4.563 ± 0.797
3.911AspLeu: 3.911 ± 0.898
2.049AspMet: 2.049 ± 0.349
3.818AspAsn: 3.818 ± 0.8
1.304AspPro: 1.304 ± 0.344
1.769AspGln: 1.769 ± 0.338
2.514AspArg: 2.514 ± 0.531
3.259AspSer: 3.259 ± 0.941
3.539AspThr: 3.539 ± 0.529
3.911AspVal: 3.911 ± 0.874
0.931AspTrp: 0.931 ± 0.335
3.352AspTyr: 3.352 ± 0.782
0.0AspXaa: 0.0 ± 0.0
Glu
4.377GluAla: 4.377 ± 0.644
0.186GluCys: 0.186 ± 0.141
3.259GluAsp: 3.259 ± 0.789
5.68GluGlu: 5.68 ± 1.266
3.259GluPhe: 3.259 ± 0.724
3.352GluGly: 3.352 ± 0.429
1.024GluHis: 1.024 ± 0.322
4.377GluIle: 4.377 ± 0.945
4.563GluLys: 4.563 ± 0.885
6.798GluLeu: 6.798 ± 0.978
1.862GluMet: 1.862 ± 0.497
5.122GluAsn: 5.122 ± 0.981
1.769GluPro: 1.769 ± 0.533
2.98GluGln: 2.98 ± 0.703
2.887GluArg: 2.887 ± 0.56
3.073GluSer: 3.073 ± 0.472
3.725GluThr: 3.725 ± 0.681
5.494GluVal: 5.494 ± 0.828
1.117GluTrp: 1.117 ± 0.321
2.98GluTyr: 2.98 ± 0.736
0.0GluXaa: 0.0 ± 0.0
Phe
2.235PheAla: 2.235 ± 0.405
0.372PheCys: 0.372 ± 0.166
3.911PheAsp: 3.911 ± 0.792
3.725PheGlu: 3.725 ± 0.653
1.49PhePhe: 1.49 ± 0.254
3.445PheGly: 3.445 ± 0.698
0.466PheHis: 0.466 ± 0.203
2.607PheIle: 2.607 ± 0.475
3.911PheLys: 3.911 ± 0.785
2.049PheLeu: 2.049 ± 0.467
1.117PheMet: 1.117 ± 0.349
4.097PheAsn: 4.097 ± 0.668
0.466PhePro: 0.466 ± 0.156
1.397PheGln: 1.397 ± 0.363
1.397PheArg: 1.397 ± 0.354
3.073PheSer: 3.073 ± 0.732
2.328PheThr: 2.328 ± 0.372
2.328PheVal: 2.328 ± 0.414
0.186PheTrp: 0.186 ± 0.132
1.955PheTyr: 1.955 ± 0.598
0.0PheXaa: 0.0 ± 0.0
Gly
4.377GlyAla: 4.377 ± 1.115
0.372GlyCys: 0.372 ± 0.21
4.097GlyAsp: 4.097 ± 0.717
3.632GlyGlu: 3.632 ± 0.677
3.259GlyPhe: 3.259 ± 0.589
2.794GlyGly: 2.794 ± 0.458
0.931GlyHis: 0.931 ± 0.353
7.449GlyIle: 7.449 ± 1.833
5.401GlyLys: 5.401 ± 0.692
5.773GlyLeu: 5.773 ± 1.037
2.142GlyMet: 2.142 ± 0.711
3.445GlyAsn: 3.445 ± 0.759
0.279GlyPro: 0.279 ± 0.133
2.328GlyGln: 2.328 ± 0.445
2.235GlyArg: 2.235 ± 0.501
4.19GlySer: 4.19 ± 0.889
4.935GlyThr: 4.935 ± 1.276
2.235GlyVal: 2.235 ± 0.465
0.745GlyTrp: 0.745 ± 0.237
2.328GlyTyr: 2.328 ± 0.632
0.0GlyXaa: 0.0 ± 0.0
His
0.372HisAla: 0.372 ± 0.196
0.093HisCys: 0.093 ± 0.094
0.745HisAsp: 0.745 ± 0.257
0.838HisGlu: 0.838 ± 0.26
0.652HisPhe: 0.652 ± 0.251
1.024HisGly: 1.024 ± 0.323
0.466HisHis: 0.466 ± 0.185
0.838HisIle: 0.838 ± 0.287
0.931HisLys: 0.931 ± 0.294
0.652HisLeu: 0.652 ± 0.19
0.372HisMet: 0.372 ± 0.166
0.372HisAsn: 0.372 ± 0.215
0.745HisPro: 0.745 ± 0.24
0.372HisGln: 0.372 ± 0.201
0.652HisArg: 0.652 ± 0.213
0.745HisSer: 0.745 ± 0.199
0.745HisThr: 0.745 ± 0.31
0.838HisVal: 0.838 ± 0.265
0.279HisTrp: 0.279 ± 0.149
0.279HisTyr: 0.279 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
6.798IleAla: 6.798 ± 1.397
0.279IleCys: 0.279 ± 0.174
5.494IleAsp: 5.494 ± 0.726
4.283IleGlu: 4.283 ± 0.68
1.211IlePhe: 1.211 ± 0.406
5.401IleGly: 5.401 ± 1.502
0.838IleHis: 0.838 ± 0.39
4.47IleIle: 4.47 ± 1.184
8.101IleLys: 8.101 ± 0.691
4.004IleLeu: 4.004 ± 0.62
1.676IleMet: 1.676 ± 0.455
5.215IleAsn: 5.215 ± 0.924
2.794IlePro: 2.794 ± 0.507
2.142IleGln: 2.142 ± 0.4
3.445IleArg: 3.445 ± 0.616
6.611IleSer: 6.611 ± 1.224
4.935IleThr: 4.935 ± 0.753
3.632IleVal: 3.632 ± 0.471
0.466IleTrp: 0.466 ± 0.236
2.421IleTyr: 2.421 ± 0.698
0.0IleXaa: 0.0 ± 0.0
Lys
6.518LysAla: 6.518 ± 0.693
0.652LysCys: 0.652 ± 0.269
3.632LysAsp: 3.632 ± 0.564
6.332LysGlu: 6.332 ± 1.019
2.7LysPhe: 2.7 ± 0.563
4.656LysGly: 4.656 ± 0.428
0.745LysHis: 0.745 ± 0.307
4.563LysIle: 4.563 ± 0.695
6.146LysLys: 6.146 ± 1.04
5.866LysLeu: 5.866 ± 0.783
2.235LysMet: 2.235 ± 0.468
4.097LysAsn: 4.097 ± 0.621
3.166LysPro: 3.166 ± 0.584
3.539LysGln: 3.539 ± 0.641
4.283LysArg: 4.283 ± 0.659
6.146LysSer: 6.146 ± 0.635
6.518LysThr: 6.518 ± 0.839
4.935LysVal: 4.935 ± 0.718
0.931LysTrp: 0.931 ± 0.319
3.259LysTyr: 3.259 ± 0.736
0.0LysXaa: 0.0 ± 0.0
Leu
5.96LeuAla: 5.96 ± 1.106
0.372LeuCys: 0.372 ± 0.24
4.749LeuAsp: 4.749 ± 0.709
5.68LeuGlu: 5.68 ± 1.073
3.166LeuPhe: 3.166 ± 0.478
5.401LeuGly: 5.401 ± 0.87
0.652LeuHis: 0.652 ± 0.229
3.911LeuIle: 3.911 ± 0.614
7.077LeuLys: 7.077 ± 0.908
4.283LeuLeu: 4.283 ± 0.764
1.583LeuMet: 1.583 ± 0.483
5.122LeuAsn: 5.122 ± 0.646
2.7LeuPro: 2.7 ± 0.584
3.166LeuGln: 3.166 ± 0.59
3.166LeuArg: 3.166 ± 0.666
5.96LeuSer: 5.96 ± 0.791
5.308LeuThr: 5.308 ± 0.739
4.283LeuVal: 4.283 ± 0.563
0.745LeuTrp: 0.745 ± 0.294
2.607LeuTyr: 2.607 ± 0.568
0.0LeuXaa: 0.0 ± 0.0
Met
2.607MetAla: 2.607 ± 0.883
0.093MetCys: 0.093 ± 0.096
1.117MetAsp: 1.117 ± 0.624
1.211MetGlu: 1.211 ± 0.224
1.211MetPhe: 1.211 ± 0.274
1.769MetGly: 1.769 ± 0.381
0.466MetHis: 0.466 ± 0.235
2.794MetIle: 2.794 ± 0.533
2.794MetLys: 2.794 ± 0.366
2.235MetLeu: 2.235 ± 0.609
0.931MetMet: 0.931 ± 0.448
1.304MetAsn: 1.304 ± 0.282
0.652MetPro: 0.652 ± 0.207
1.49MetGln: 1.49 ± 0.56
1.304MetArg: 1.304 ± 0.388
2.049MetSer: 2.049 ± 0.482
2.049MetThr: 2.049 ± 0.506
2.421MetVal: 2.421 ± 0.582
0.372MetTrp: 0.372 ± 0.218
0.838MetTyr: 0.838 ± 0.399
0.0MetXaa: 0.0 ± 0.0
Asn
4.283AsnAla: 4.283 ± 0.562
0.652AsnCys: 0.652 ± 0.186
4.47AsnAsp: 4.47 ± 0.775
3.632AsnGlu: 3.632 ± 0.647
2.514AsnPhe: 2.514 ± 0.599
4.004AsnGly: 4.004 ± 0.785
0.838AsnHis: 0.838 ± 0.259
4.004AsnIle: 4.004 ± 0.875
4.283AsnLys: 4.283 ± 0.757
3.818AsnLeu: 3.818 ± 0.621
1.583AsnMet: 1.583 ± 0.332
3.166AsnAsn: 3.166 ± 0.491
2.142AsnPro: 2.142 ± 0.35
2.514AsnGln: 2.514 ± 0.491
1.211AsnArg: 1.211 ± 0.378
3.725AsnSer: 3.725 ± 0.683
3.352AsnThr: 3.352 ± 0.657
3.166AsnVal: 3.166 ± 0.595
1.304AsnTrp: 1.304 ± 0.344
2.7AsnTyr: 2.7 ± 0.576
0.0AsnXaa: 0.0 ± 0.0
Pro
0.931ProAla: 0.931 ± 0.367
0.0ProCys: 0.0 ± 0.0
1.676ProAsp: 1.676 ± 0.353
2.328ProGlu: 2.328 ± 0.481
1.676ProPhe: 1.676 ± 0.372
1.304ProGly: 1.304 ± 0.358
0.279ProHis: 0.279 ± 0.172
2.049ProIle: 2.049 ± 0.437
2.887ProLys: 2.887 ± 0.578
2.235ProLeu: 2.235 ± 0.689
0.279ProMet: 0.279 ± 0.168
1.49ProAsn: 1.49 ± 0.518
0.931ProPro: 0.931 ± 0.278
1.304ProGln: 1.304 ± 0.337
1.117ProArg: 1.117 ± 0.352
2.142ProSer: 2.142 ± 0.486
2.235ProThr: 2.235 ± 0.504
2.142ProVal: 2.142 ± 0.471
0.093ProTrp: 0.093 ± 0.102
1.583ProTyr: 1.583 ± 0.469
0.0ProXaa: 0.0 ± 0.0
Gln
4.656GlnAla: 4.656 ± 1.177
0.372GlnCys: 0.372 ± 0.153
1.211GlnAsp: 1.211 ± 0.278
2.328GlnGlu: 2.328 ± 0.547
2.142GlnPhe: 2.142 ± 0.393
2.328GlnGly: 2.328 ± 0.72
0.372GlnHis: 0.372 ± 0.188
3.539GlnIle: 3.539 ± 0.795
2.887GlnLys: 2.887 ± 0.468
4.004GlnLeu: 4.004 ± 0.828
2.142GlnMet: 2.142 ± 0.429
1.676GlnAsn: 1.676 ± 0.397
0.279GlnPro: 0.279 ± 0.154
2.328GlnGln: 2.328 ± 0.433
1.117GlnArg: 1.117 ± 0.292
4.004GlnSer: 4.004 ± 0.557
1.769GlnThr: 1.769 ± 0.285
2.142GlnVal: 2.142 ± 0.455
0.559GlnTrp: 0.559 ± 0.199
0.931GlnTyr: 0.931 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
1.769ArgAla: 1.769 ± 0.309
0.186ArgCys: 0.186 ± 0.124
2.887ArgAsp: 2.887 ± 0.431
3.073ArgGlu: 3.073 ± 0.599
1.211ArgPhe: 1.211 ± 0.365
2.049ArgGly: 2.049 ± 0.405
0.745ArgHis: 0.745 ± 0.244
2.049ArgIle: 2.049 ± 0.433
3.539ArgLys: 3.539 ± 0.774
3.073ArgLeu: 3.073 ± 0.603
1.583ArgMet: 1.583 ± 0.441
2.7ArgAsn: 2.7 ± 0.56
1.211ArgPro: 1.211 ± 0.428
1.211ArgGln: 1.211 ± 0.29
1.769ArgArg: 1.769 ± 0.501
1.862ArgSer: 1.862 ± 0.319
2.235ArgThr: 2.235 ± 0.558
2.887ArgVal: 2.887 ± 0.756
0.652ArgTrp: 0.652 ± 0.245
1.955ArgTyr: 1.955 ± 0.444
0.0ArgXaa: 0.0 ± 0.0
Ser
5.96SerAla: 5.96 ± 2.918
0.372SerCys: 0.372 ± 0.188
3.166SerAsp: 3.166 ± 0.677
4.283SerGlu: 4.283 ± 0.814
3.166SerPhe: 3.166 ± 0.579
5.215SerGly: 5.215 ± 0.872
0.559SerHis: 0.559 ± 0.203
6.239SerIle: 6.239 ± 0.806
5.028SerLys: 5.028 ± 0.66
6.705SerLeu: 6.705 ± 0.819
2.328SerMet: 2.328 ± 0.446
2.887SerAsn: 2.887 ± 0.576
2.142SerPro: 2.142 ± 0.375
3.352SerGln: 3.352 ± 0.826
2.235SerArg: 2.235 ± 0.539
5.773SerSer: 5.773 ± 1.233
3.445SerThr: 3.445 ± 0.782
5.494SerVal: 5.494 ± 0.737
0.559SerTrp: 0.559 ± 0.254
1.583SerTyr: 1.583 ± 0.453
0.0SerXaa: 0.0 ± 0.0
Thr
5.215ThrAla: 5.215 ± 1.241
0.279ThrCys: 0.279 ± 0.154
4.377ThrAsp: 4.377 ± 0.812
3.911ThrGlu: 3.911 ± 0.729
2.98ThrPhe: 2.98 ± 0.555
3.539ThrGly: 3.539 ± 0.747
0.652ThrHis: 0.652 ± 0.272
4.749ThrIle: 4.749 ± 0.627
4.749ThrLys: 4.749 ± 0.769
5.215ThrLeu: 5.215 ± 0.767
1.583ThrMet: 1.583 ± 0.629
2.98ThrAsn: 2.98 ± 0.666
2.328ThrPro: 2.328 ± 0.412
3.073ThrGln: 3.073 ± 0.793
1.49ThrArg: 1.49 ± 0.464
4.842ThrSer: 4.842 ± 1.173
4.004ThrThr: 4.004 ± 0.716
4.749ThrVal: 4.749 ± 0.515
0.279ThrTrp: 0.279 ± 0.146
3.259ThrTyr: 3.259 ± 0.675
0.0ThrXaa: 0.0 ± 0.0
Val
5.028ValAla: 5.028 ± 0.661
0.279ValCys: 0.279 ± 0.143
3.259ValAsp: 3.259 ± 0.69
4.935ValGlu: 4.935 ± 0.866
2.794ValPhe: 2.794 ± 0.482
3.911ValGly: 3.911 ± 0.935
0.652ValHis: 0.652 ± 0.274
5.494ValIle: 5.494 ± 0.607
4.19ValLys: 4.19 ± 0.641
3.352ValLeu: 3.352 ± 0.792
1.583ValMet: 1.583 ± 0.336
2.794ValAsn: 2.794 ± 0.566
1.769ValPro: 1.769 ± 0.356
2.235ValGln: 2.235 ± 0.577
1.955ValArg: 1.955 ± 0.347
4.842ValSer: 4.842 ± 0.782
4.842ValThr: 4.842 ± 0.681
4.749ValVal: 4.749 ± 0.664
0.838ValTrp: 0.838 ± 0.344
2.794ValTyr: 2.794 ± 0.621
0.0ValXaa: 0.0 ± 0.0
Trp
0.372TrpAla: 0.372 ± 0.183
0.093TrpCys: 0.093 ± 0.089
0.838TrpAsp: 0.838 ± 0.305
0.745TrpGlu: 0.745 ± 0.286
0.652TrpPhe: 0.652 ± 0.242
0.279TrpGly: 0.279 ± 0.165
0.186TrpHis: 0.186 ± 0.132
0.652TrpIle: 0.652 ± 0.245
0.559TrpLys: 0.559 ± 0.19
1.304TrpLeu: 1.304 ± 0.291
0.372TrpMet: 0.372 ± 0.203
0.745TrpAsn: 0.745 ± 0.215
0.186TrpPro: 0.186 ± 0.133
0.838TrpGln: 0.838 ± 0.241
0.745TrpArg: 0.745 ± 0.323
0.652TrpSer: 0.652 ± 0.274
0.931TrpThr: 0.931 ± 0.369
0.652TrpVal: 0.652 ± 0.266
0.093TrpTrp: 0.093 ± 0.082
0.559TrpTyr: 0.559 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.142TyrAla: 2.142 ± 0.468
0.372TyrCys: 0.372 ± 0.197
2.794TyrAsp: 2.794 ± 0.667
3.259TyrGlu: 3.259 ± 0.731
2.049TyrPhe: 2.049 ± 0.646
2.7TyrGly: 2.7 ± 0.559
0.372TyrHis: 0.372 ± 0.167
2.607TyrIle: 2.607 ± 0.506
2.235TyrLys: 2.235 ± 0.571
4.283TyrLeu: 4.283 ± 0.838
1.117TyrMet: 1.117 ± 0.363
1.862TyrAsn: 1.862 ± 0.514
0.931TyrPro: 0.931 ± 0.332
1.862TyrGln: 1.862 ± 0.528
2.328TyrArg: 2.328 ± 0.805
2.98TyrSer: 2.98 ± 0.563
1.583TyrThr: 1.583 ± 0.481
1.769TyrVal: 1.769 ± 0.477
0.652TyrTrp: 0.652 ± 0.245
2.794TyrTyr: 2.794 ± 0.809
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (10740 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski