Staphylococcus virus 47
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZCH3|Q4ZCH3_9CAUD ORF048 OS=Staphylococcus virus 47 OX=320839 PE=4 SV=1
MM1 pKa = 7.06 ATQRR5 pKa = 11.84 QVEE8 pKa = 4.5 YY9 pKa = 11.4 VMSLQEE15 pKa = 3.92 QLEE18 pKa = 4.35 LEE20 pKa = 4.45 DD21 pKa = 3.95 CEE23 pKa = 5.56 KK24 pKa = 10.67 YY25 pKa = 9.42 TDD27 pKa = 3.83 EE28 pKa = 4.01 QVKK31 pKa = 10.4 AMSHH35 pKa = 6.03 KK36 pKa = 10.06 EE37 pKa = 3.72 VSNVIEE43 pKa = 4.41 NYY45 pKa = 9.42 KK46 pKa = 10.77 ASISNEE52 pKa = 3.8 EE53 pKa = 4.63 LYY55 pKa = 10.85 DD56 pKa = 3.48 EE57 pKa = 4.93 CMSFGLPNCC66 pKa = 4.51
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.045
IPC2_protein 4.342
IPC_protein 4.177
Toseland 4.037
ProMoST 4.266
Dawson 4.101
Bjellqvist 4.253
Wikipedia 3.948
Rodwell 4.012
Grimsley 3.948
Solomon 4.088
Lehninger 4.037
Nozaki 4.228
DTASelect 4.279
Thurlkill 4.037
EMBOSS 3.973
Sillero 4.279
Patrickios 0.846
IPC_peptide 4.101
IPC2_peptide 4.266
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|Q4ZCJ8|Q4ZCJ8_9CAUD ORF014 OS=Staphylococcus virus 47 OX=320839 PE=4 SV=1
MM1 pKa = 7.51 FFFRR5 pKa = 11.84 GEE7 pKa = 3.53 KK8 pKa = 9.04 RR9 pKa = 11.84 ANYY12 pKa = 9.33 LNKK15 pKa = 10.25 GKK17 pKa = 9.53 HH18 pKa = 4.18 AWKK21 pKa = 8.53 TQRR24 pKa = 11.84 FKK26 pKa = 11.15 NAKK29 pKa = 7.45 TVDD32 pKa = 3.57 MTRR35 pKa = 11.84 FLYY38 pKa = 10.71 VCIQRR43 pKa = 11.84 RR44 pKa = 11.84 IIYY47 pKa = 9.97 SPSHH51 pKa = 6.41 YY52 pKa = 10.88 DD53 pKa = 4.0 DD54 pKa = 4.0 MFNSRR59 pKa = 11.84 KK60 pKa = 10.03 ALTPVFSSVLL70 pKa = 3.48
Molecular weight: 8.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.882
IPC_protein 10.423
Toseland 10.599
ProMoST 10.233
Dawson 10.73
Bjellqvist 10.394
Wikipedia 10.906
Rodwell 11.155
Grimsley 10.789
Solomon 10.789
Lehninger 10.76
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.643
Patrickios 10.906
IPC_peptide 10.789
IPC2_peptide 9.238
IPC2.peptide.svr19 8.518
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
14332
34
2066
199.1
22.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.777 ± 0.58
0.621 ± 0.13
6.126 ± 0.289
7.675 ± 0.448
3.838 ± 0.227
5.91 ± 0.547
1.814 ± 0.109
7.082 ± 0.339
9.866 ± 0.467
8.547 ± 0.295
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.561 ± 0.123
6.294 ± 0.255
2.651 ± 0.221
3.586 ± 0.181
4.061 ± 0.223
6.405 ± 0.326
5.91 ± 0.252
5.735 ± 0.223
1.242 ± 0.122
4.298 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here