Hydrogenophaga sp. PBC

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga; unclassified Hydrogenophaga

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9VGZ9|A0A1C9VGZ9_9BURK Acetyltransferase OS=Hydrogenophaga sp. PBC OX=795665 GN=Q5W_14075 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 4.81EE3 pKa = 5.13PDD5 pKa = 3.62LFTWLQHH12 pKa = 4.61WRR14 pKa = 11.84CNTGHH19 pKa = 6.8EE20 pKa = 4.16AQAAQAMPQVEE31 pKa = 4.36PLALQVGDD39 pKa = 3.26AWMALSTTQQPDD51 pKa = 3.69VIRR54 pKa = 11.84VDD56 pKa = 3.71VLIEE60 pKa = 3.74PVLEE64 pKa = 4.39HH65 pKa = 6.96EE66 pKa = 4.46VAPWICEE73 pKa = 3.69EE74 pKa = 4.02LLRR77 pKa = 11.84WSFGHH82 pKa = 7.23PYY84 pKa = 10.62LSDD87 pKa = 3.51GLQFALDD94 pKa = 3.69PDD96 pKa = 4.4GALVACAQLAIEE108 pKa = 5.05PGMEE112 pKa = 3.88APAVEE117 pKa = 4.4EE118 pKa = 4.23LLMEE122 pKa = 4.17TAEE125 pKa = 4.49QINEE129 pKa = 3.52AWTLIMADD137 pKa = 4.44ALLQAYY143 pKa = 7.92LAQQPPTAVQGSLAVV158 pKa = 3.44

Molecular weight: 17.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9VKA5|A0A1C9VKA5_9BURK Lipoprotein signal peptidase OS=Hydrogenophaga sp. PBC OX=795665 GN=lspA PE=3 SV=1
MM1 pKa = 7.92IGISPRR7 pKa = 11.84ALISSMAALLALLLSRR23 pKa = 11.84VTFSGTPLAFMALSKK38 pKa = 10.74KK39 pKa = 8.71RR40 pKa = 11.84TAAALSRR47 pKa = 11.84WAVSRR52 pKa = 11.84KK53 pKa = 8.05STVFPSLSTARR64 pKa = 3.86

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4855

0

4855

1570076

27

4199

323.4

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.168 ± 0.05

0.863 ± 0.009

5.298 ± 0.024

5.34 ± 0.03

3.395 ± 0.018

8.489 ± 0.031

2.387 ± 0.019

3.994 ± 0.024

2.869 ± 0.036

11.05 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.018

2.434 ± 0.022

5.509 ± 0.027

3.982 ± 0.023

7.454 ± 0.035

5.013 ± 0.024

5.061 ± 0.024

7.711 ± 0.027

1.59 ± 0.017

2.017 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski