Dialister sp. CAG:486
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1926 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6A8G7|R6A8G7_9FIRM Uncharacterized protein OS=Dialister sp. CAG:486 OX=1262870 GN=BN678_01231 PE=3 SV=1
MM1 pKa = 7.31 TSASVRR7 pKa = 11.84 TDD9 pKa = 3.68 FYY11 pKa = 10.73 QTFNVEE17 pKa = 4.61 GFFSTKK23 pKa = 7.58 VTFDD27 pKa = 4.31 FIISEE32 pKa = 4.35 YY33 pKa = 11.0 AADD36 pKa = 3.44 LCYY39 pKa = 10.29 IIFRR43 pKa = 11.84 QISDD47 pKa = 3.51 ADD49 pKa = 3.59 VSINSCFAA57 pKa = 4.03
Molecular weight: 6.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.846
IPC2_protein 4.088
IPC_protein 3.91
Toseland 3.706
ProMoST 4.113
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.846
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.037
Patrickios 0.604
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|R6AUQ8|R6AUQ8_9FIRM Signal peptidase I OS=Dialister sp. CAG:486 OX=1262870 GN=BN678_00201 PE=3 SV=1
MM1 pKa = 7.7 AKK3 pKa = 7.99 MTFQPNNHH11 pKa = 5.63 WRR13 pKa = 11.84 KK14 pKa = 6.87 QTHH17 pKa = 5.61 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MKK24 pKa = 8.64 TKK26 pKa = 10.33 AGRR29 pKa = 11.84 IVLKK33 pKa = 10.36 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.89 GRR40 pKa = 11.84 KK41 pKa = 8.67 VLSAA45 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1926
0
1926
572162
29
2613
297.1
32.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.071 ± 0.06
1.251 ± 0.023
5.677 ± 0.047
6.929 ± 0.066
4.02 ± 0.044
7.664 ± 0.06
2.281 ± 0.026
6.637 ± 0.049
6.36 ± 0.048
9.374 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.268 ± 0.025
3.257 ± 0.047
3.91 ± 0.04
2.94 ± 0.033
5.018 ± 0.045
5.731 ± 0.041
5.333 ± 0.04
6.777 ± 0.049
1.036 ± 0.022
3.462 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here