Sporobolus striate mosaic virus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7FHN2|J7FHN2_9GEMI Capsid protein OS=Sporobolus striate mosaic virus 2 OX=1302850 PE=3 SV=1
MM1 pKa = 7.66SSQSNSTEE9 pKa = 3.45ASPANFRR16 pKa = 11.84FRR18 pKa = 11.84ARR20 pKa = 11.84SAFLTYY26 pKa = 9.35PKK28 pKa = 9.65CTLEE32 pKa = 4.02PRR34 pKa = 11.84DD35 pKa = 4.05VVEE38 pKa = 4.08HH39 pKa = 6.71LYY41 pKa = 11.21SKK43 pKa = 10.1FRR45 pKa = 11.84KK46 pKa = 9.61YY47 pKa = 10.39GPKK50 pKa = 8.48YY51 pKa = 9.44CLVTRR56 pKa = 11.84EE57 pKa = 4.04HH58 pKa = 6.9HH59 pKa = 7.16SDD61 pKa = 3.16GDD63 pKa = 3.87YY64 pKa = 11.08HH65 pKa = 6.47LHH67 pKa = 7.29CLFQLDD73 pKa = 4.43KK74 pKa = 11.4AFSTNDD80 pKa = 2.79SSTFNILDD88 pKa = 3.54YY89 pKa = 11.01HH90 pKa = 6.63PNIQTAKK97 pKa = 10.19SPTNVRR103 pKa = 11.84DD104 pKa = 3.61YY105 pKa = 11.11CLKK108 pKa = 10.68NPVSKK113 pKa = 11.01AEE115 pKa = 4.09RR116 pKa = 11.84GTFIPLKK123 pKa = 10.62GRR125 pKa = 11.84TPKK128 pKa = 9.28NTEE131 pKa = 3.78SKK133 pKa = 11.03AKK135 pKa = 10.03DD136 pKa = 3.24SVMRR140 pKa = 11.84SIINTSTDD148 pKa = 3.14RR149 pKa = 11.84ASYY152 pKa = 11.0LSMVRR157 pKa = 11.84KK158 pKa = 9.91AFPFDD163 pKa = 3.26WATKK167 pKa = 8.85LQQFEE172 pKa = 4.39YY173 pKa = 10.11SASKK177 pKa = 10.47LFPDD181 pKa = 4.99VIPEE185 pKa = 4.06YY186 pKa = 9.9TSPFPTEE193 pKa = 4.11NLMCNEE199 pKa = 5.62RR200 pKa = 11.84ITDD203 pKa = 3.49WLDD206 pKa = 2.99NTLYY210 pKa = 10.34QVSPAAYY217 pKa = 10.01SSLHH221 pKa = 5.59PQADD225 pKa = 3.27ASGDD229 pKa = 4.14LNWLMDD235 pKa = 4.08ASLQTIQEE243 pKa = 4.25LGNLGSTSADD253 pKa = 3.04QIVPVRR259 pKa = 11.84HH260 pKa = 6.52LGPGVV265 pKa = 3.14

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7FGY7|J7FGY7_9GEMI Replication-associated protein OS=Sporobolus striate mosaic virus 2 OX=1302850 PE=3 SV=1
MM1 pKa = 7.59SNPATPLGDD10 pKa = 3.68YY11 pKa = 9.15TSSQHH16 pKa = 6.31QGGTQSGSVGIGNDD30 pKa = 3.66SAWRR34 pKa = 11.84ALALAFTVTTVTLVLLFGAWRR55 pKa = 11.84LCLKK59 pKa = 10.71DD60 pKa = 3.77CLLTLRR66 pKa = 11.84AKK68 pKa = 10.38RR69 pKa = 11.84SKK71 pKa = 7.92TTTEE75 pKa = 4.0LGFGQTPRR83 pKa = 11.84RR84 pKa = 11.84DD85 pKa = 3.57PVGGGVTQYY94 pKa = 11.04PPGVPGG100 pKa = 3.64

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

957

100

346

239.3

27.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.956 ± 0.37

1.881 ± 0.084

5.747 ± 0.375

3.657 ± 0.655

4.598 ± 0.346

6.688 ± 1.992

2.299 ± 0.366

3.866 ± 0.518

5.852 ± 0.594

7.315 ± 1.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.985 ± 0.248

5.225 ± 0.754

6.165 ± 0.28

3.239 ± 0.388

6.688 ± 1.005

8.986 ± 0.832

7.837 ± 0.884

5.12 ± 0.848

1.776 ± 0.275

5.12 ± 0.577
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski