Amino acid dipepetide frequency for Gordonia phage Bowser

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.362AlaAla: 18.362 ± 1.491
0.685AlaCys: 0.685 ± 0.261
8.359AlaAsp: 8.359 ± 0.742
8.085AlaGlu: 8.085 ± 0.925
3.083AlaPhe: 3.083 ± 0.517
10.963AlaGly: 10.963 ± 1.074
2.055AlaHis: 2.055 ± 0.371
4.796AlaIle: 4.796 ± 0.649
3.974AlaLys: 3.974 ± 0.611
10.072AlaLeu: 10.072 ± 0.839
2.946AlaMet: 2.946 ± 0.549
3.426AlaAsn: 3.426 ± 0.456
7.263AlaPro: 7.263 ± 0.934
4.454AlaGln: 4.454 ± 0.69
9.318AlaArg: 9.318 ± 0.902
6.715AlaSer: 6.715 ± 0.798
8.496AlaThr: 8.496 ± 0.865
8.565AlaVal: 8.565 ± 0.908
2.261AlaTrp: 2.261 ± 0.406
1.918AlaTyr: 1.918 ± 0.313
0.0AlaXaa: 0.0 ± 0.0
Cys
0.548CysAla: 0.548 ± 0.243
0.069CysCys: 0.069 ± 0.067
0.822CysAsp: 0.822 ± 0.227
0.548CysGlu: 0.548 ± 0.207
0.069CysPhe: 0.069 ± 0.062
0.754CysGly: 0.754 ± 0.271
0.206CysHis: 0.206 ± 0.121
0.206CysIle: 0.206 ± 0.108
0.069CysLys: 0.069 ± 0.069
0.617CysLeu: 0.617 ± 0.245
0.069CysMet: 0.069 ± 0.09
0.411CysAsn: 0.411 ± 0.151
0.891CysPro: 0.891 ± 0.288
0.206CysGln: 0.206 ± 0.106
0.754CysArg: 0.754 ± 0.24
0.48CysSer: 0.48 ± 0.185
0.617CysThr: 0.617 ± 0.193
0.411CysVal: 0.411 ± 0.164
0.411CysTrp: 0.411 ± 0.149
0.137CysTyr: 0.137 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
7.811AspAla: 7.811 ± 0.665
0.891AspCys: 0.891 ± 0.261
5.002AspAsp: 5.002 ± 0.766
5.961AspGlu: 5.961 ± 0.675
2.398AspPhe: 2.398 ± 0.374
6.372AspGly: 6.372 ± 0.744
1.85AspHis: 1.85 ± 0.406
1.918AspIle: 1.918 ± 0.471
1.439AspLys: 1.439 ± 0.376
6.029AspLeu: 6.029 ± 0.763
0.959AspMet: 0.959 ± 0.239
1.302AspAsn: 1.302 ± 0.306
4.591AspPro: 4.591 ± 0.784
2.535AspGln: 2.535 ± 0.395
4.591AspArg: 4.591 ± 0.812
3.7AspSer: 3.7 ± 0.48
2.946AspThr: 2.946 ± 0.495
4.042AspVal: 4.042 ± 0.674
1.439AspTrp: 1.439 ± 0.273
1.37AspTyr: 1.37 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
6.235GluAla: 6.235 ± 0.744
0.548GluCys: 0.548 ± 0.279
3.083GluAsp: 3.083 ± 0.449
2.398GluGlu: 2.398 ± 0.427
2.055GluPhe: 2.055 ± 0.565
4.522GluGly: 4.522 ± 0.584
1.096GluHis: 1.096 ± 0.302
3.289GluIle: 3.289 ± 0.529
1.576GluLys: 1.576 ± 0.455
5.55GluLeu: 5.55 ± 0.693
1.096GluMet: 1.096 ± 0.292
1.302GluAsn: 1.302 ± 0.354
3.7GluPro: 3.7 ± 0.67
2.124GluGln: 2.124 ± 0.34
5.002GluArg: 5.002 ± 0.714
3.22GluSer: 3.22 ± 0.433
3.7GluThr: 3.7 ± 0.482
3.631GluVal: 3.631 ± 0.525
1.165GluTrp: 1.165 ± 0.333
1.918GluTyr: 1.918 ± 0.337
0.0GluXaa: 0.0 ± 0.0
Phe
2.809PheAla: 2.809 ± 0.462
0.137PheCys: 0.137 ± 0.105
1.987PheAsp: 1.987 ± 0.376
2.398PheGlu: 2.398 ± 0.424
0.685PhePhe: 0.685 ± 0.243
2.672PheGly: 2.672 ± 0.496
0.891PheHis: 0.891 ± 0.29
1.233PheIle: 1.233 ± 0.305
0.411PheLys: 0.411 ± 0.186
2.33PheLeu: 2.33 ± 0.489
0.069PheMet: 0.069 ± 0.055
0.617PheAsn: 0.617 ± 0.238
1.233PhePro: 1.233 ± 0.295
0.754PheGln: 0.754 ± 0.195
1.576PheArg: 1.576 ± 0.345
1.576PheSer: 1.576 ± 0.401
2.055PheThr: 2.055 ± 0.445
2.193PheVal: 2.193 ± 0.35
0.617PheTrp: 0.617 ± 0.186
0.685PheTyr: 0.685 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
9.113GlyAla: 9.113 ± 1.159
0.48GlyCys: 0.48 ± 0.176
5.481GlyAsp: 5.481 ± 0.771
4.728GlyGlu: 4.728 ± 0.531
2.741GlyPhe: 2.741 ± 0.438
8.428GlyGly: 8.428 ± 0.959
2.261GlyHis: 2.261 ± 0.312
3.7GlyIle: 3.7 ± 0.687
2.467GlyLys: 2.467 ± 0.341
7.537GlyLeu: 7.537 ± 0.762
2.055GlyMet: 2.055 ± 0.351
2.398GlyAsn: 2.398 ± 0.476
4.18GlyPro: 4.18 ± 0.557
2.946GlyGln: 2.946 ± 0.443
7.194GlyArg: 7.194 ± 0.716
4.591GlySer: 4.591 ± 0.551
5.207GlyThr: 5.207 ± 0.573
7.126GlyVal: 7.126 ± 0.699
1.439GlyTrp: 1.439 ± 0.291
2.809GlyTyr: 2.809 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
2.193HisAla: 2.193 ± 0.387
0.343HisCys: 0.343 ± 0.19
1.096HisAsp: 1.096 ± 0.245
0.959HisGlu: 0.959 ± 0.24
0.48HisPhe: 0.48 ± 0.243
1.576HisGly: 1.576 ± 0.417
0.548HisHis: 0.548 ± 0.182
0.959HisIle: 0.959 ± 0.272
0.274HisLys: 0.274 ± 0.132
1.918HisLeu: 1.918 ± 0.427
0.274HisMet: 0.274 ± 0.143
0.617HisAsn: 0.617 ± 0.21
1.713HisPro: 1.713 ± 0.303
0.548HisGln: 0.548 ± 0.21
1.233HisArg: 1.233 ± 0.305
1.37HisSer: 1.37 ± 0.27
0.959HisThr: 0.959 ± 0.287
1.439HisVal: 1.439 ± 0.323
0.411HisTrp: 0.411 ± 0.155
0.48HisTyr: 0.48 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
6.646IleAla: 6.646 ± 0.747
0.274IleCys: 0.274 ± 0.115
2.946IleAsp: 2.946 ± 0.492
3.22IleGlu: 3.22 ± 0.565
0.822IlePhe: 0.822 ± 0.263
3.768IleGly: 3.768 ± 0.601
0.754IleHis: 0.754 ± 0.241
1.918IleIle: 1.918 ± 0.336
1.302IleLys: 1.302 ± 0.488
2.467IleLeu: 2.467 ± 0.412
0.411IleMet: 0.411 ± 0.176
1.439IleAsn: 1.439 ± 0.389
2.124IlePro: 2.124 ± 0.398
1.37IleGln: 1.37 ± 0.381
3.289IleArg: 3.289 ± 0.486
2.809IleSer: 2.809 ± 0.452
3.083IleThr: 3.083 ± 0.418
3.357IleVal: 3.357 ± 0.492
0.959IleTrp: 0.959 ± 0.279
1.096IleTyr: 1.096 ± 0.276
0.0IleXaa: 0.0 ± 0.0
Lys
3.426LysAla: 3.426 ± 0.496
0.206LysCys: 0.206 ± 0.107
2.055LysAsp: 2.055 ± 0.496
0.822LysGlu: 0.822 ± 0.237
0.891LysPhe: 0.891 ± 0.298
2.467LysGly: 2.467 ± 0.381
0.206LysHis: 0.206 ± 0.127
1.37LysIle: 1.37 ± 0.333
1.644LysLys: 1.644 ± 0.562
2.33LysLeu: 2.33 ± 0.522
0.48LysMet: 0.48 ± 0.184
0.548LysAsn: 0.548 ± 0.174
1.439LysPro: 1.439 ± 0.309
0.891LysGln: 0.891 ± 0.313
1.781LysArg: 1.781 ± 0.459
1.644LysSer: 1.644 ± 0.37
2.261LysThr: 2.261 ± 0.469
2.055LysVal: 2.055 ± 0.313
0.959LysTrp: 0.959 ± 0.332
0.48LysTyr: 0.48 ± 0.182
0.0LysXaa: 0.0 ± 0.0
Leu
11.442LeuAla: 11.442 ± 0.846
0.48LeuCys: 0.48 ± 0.188
5.481LeuAsp: 5.481 ± 0.705
4.317LeuGlu: 4.317 ± 0.792
2.398LeuPhe: 2.398 ± 0.391
6.235LeuGly: 6.235 ± 0.625
1.781LeuHis: 1.781 ± 0.363
3.152LeuIle: 3.152 ± 0.466
1.644LeuLys: 1.644 ± 0.333
6.509LeuLeu: 6.509 ± 0.836
1.713LeuMet: 1.713 ± 0.341
2.124LeuAsn: 2.124 ± 0.312
5.276LeuPro: 5.276 ± 0.737
2.33LeuGln: 2.33 ± 0.451
6.441LeuArg: 6.441 ± 0.797
5.002LeuSer: 5.002 ± 0.653
5.892LeuThr: 5.892 ± 0.72
7.263LeuVal: 7.263 ± 0.803
1.37LeuTrp: 1.37 ± 0.323
1.507LeuTyr: 1.507 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
3.426MetAla: 3.426 ± 0.78
0.137MetCys: 0.137 ± 0.095
1.233MetAsp: 1.233 ± 0.266
0.343MetGlu: 0.343 ± 0.146
0.48MetPhe: 0.48 ± 0.197
1.576MetGly: 1.576 ± 0.41
0.343MetHis: 0.343 ± 0.162
1.233MetIle: 1.233 ± 0.324
0.411MetLys: 0.411 ± 0.163
2.33MetLeu: 2.33 ± 0.395
0.685MetMet: 0.685 ± 0.209
0.754MetAsn: 0.754 ± 0.24
1.165MetPro: 1.165 ± 0.263
0.617MetGln: 0.617 ± 0.191
1.644MetArg: 1.644 ± 0.318
1.302MetSer: 1.302 ± 0.324
2.055MetThr: 2.055 ± 0.39
1.165MetVal: 1.165 ± 0.245
0.754MetTrp: 0.754 ± 0.259
0.137MetTyr: 0.137 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
3.494AsnAla: 3.494 ± 0.602
0.0AsnCys: 0.0 ± 0.0
1.918AsnAsp: 1.918 ± 0.279
0.822AsnGlu: 0.822 ± 0.251
0.48AsnPhe: 0.48 ± 0.192
3.563AsnGly: 3.563 ± 0.618
0.48AsnHis: 0.48 ± 0.154
0.959AsnIle: 0.959 ± 0.383
0.343AsnLys: 0.343 ± 0.202
2.261AsnLeu: 2.261 ± 0.471
0.48AsnMet: 0.48 ± 0.172
0.822AsnAsn: 0.822 ± 0.297
2.604AsnPro: 2.604 ± 0.404
1.165AsnGln: 1.165 ± 0.366
2.193AsnArg: 2.193 ± 0.426
0.959AsnSer: 0.959 ± 0.319
1.644AsnThr: 1.644 ± 0.301
1.987AsnVal: 1.987 ± 0.349
0.754AsnTrp: 0.754 ± 0.191
0.617AsnTyr: 0.617 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
8.085ProAla: 8.085 ± 0.852
0.754ProCys: 0.754 ± 0.246
4.865ProAsp: 4.865 ± 0.672
3.083ProGlu: 3.083 ± 0.479
1.507ProPhe: 1.507 ± 0.378
5.413ProGly: 5.413 ± 0.702
0.685ProHis: 0.685 ± 0.27
2.398ProIle: 2.398 ± 0.469
1.644ProLys: 1.644 ± 0.315
4.111ProLeu: 4.111 ± 0.467
1.096ProMet: 1.096 ± 0.287
2.193ProAsn: 2.193 ± 0.445
4.796ProPro: 4.796 ± 1.237
1.987ProGln: 1.987 ± 0.435
3.7ProArg: 3.7 ± 0.626
3.22ProSer: 3.22 ± 0.608
4.18ProThr: 4.18 ± 0.791
4.591ProVal: 4.591 ± 0.55
1.713ProTrp: 1.713 ± 0.438
0.822ProTyr: 0.822 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
3.768GlnAla: 3.768 ± 0.744
0.411GlnCys: 0.411 ± 0.169
1.576GlnAsp: 1.576 ± 0.344
1.37GlnGlu: 1.37 ± 0.374
0.685GlnPhe: 0.685 ± 0.177
2.604GlnGly: 2.604 ± 0.519
0.822GlnHis: 0.822 ± 0.221
1.918GlnIle: 1.918 ± 0.454
1.028GlnLys: 1.028 ± 0.298
3.357GlnLeu: 3.357 ± 0.424
1.507GlnMet: 1.507 ± 0.33
1.028GlnAsn: 1.028 ± 0.279
1.644GlnPro: 1.644 ± 0.371
1.781GlnGln: 1.781 ± 0.351
2.672GlnArg: 2.672 ± 0.503
1.713GlnSer: 1.713 ± 0.35
1.987GlnThr: 1.987 ± 0.344
2.261GlnVal: 2.261 ± 0.485
0.617GlnTrp: 0.617 ± 0.226
0.48GlnTyr: 0.48 ± 0.173
0.0GlnXaa: 0.0 ± 0.0
Arg
8.428ArgAla: 8.428 ± 0.957
0.822ArgCys: 0.822 ± 0.268
5.139ArgAsp: 5.139 ± 0.584
3.563ArgGlu: 3.563 ± 0.523
1.507ArgPhe: 1.507 ± 0.313
4.796ArgGly: 4.796 ± 0.624
1.781ArgHis: 1.781 ± 0.409
3.426ArgIle: 3.426 ± 0.519
3.22ArgLys: 3.22 ± 0.589
6.166ArgLeu: 6.166 ± 0.717
2.672ArgMet: 2.672 ± 0.44
2.535ArgAsn: 2.535 ± 0.497
4.18ArgPro: 4.18 ± 0.775
3.289ArgGln: 3.289 ± 0.58
7.4ArgArg: 7.4 ± 1.081
4.18ArgSer: 4.18 ± 0.516
4.317ArgThr: 4.317 ± 0.486
4.18ArgVal: 4.18 ± 0.516
1.302ArgTrp: 1.302 ± 0.36
1.37ArgTyr: 1.37 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
7.674SerAla: 7.674 ± 0.851
0.274SerCys: 0.274 ± 0.114
3.289SerAsp: 3.289 ± 0.423
3.631SerGlu: 3.631 ± 0.607
1.165SerPhe: 1.165 ± 0.365
6.235SerGly: 6.235 ± 0.845
0.754SerHis: 0.754 ± 0.222
3.015SerIle: 3.015 ± 0.427
1.37SerLys: 1.37 ± 0.325
3.905SerLeu: 3.905 ± 0.579
1.507SerMet: 1.507 ± 0.398
1.439SerAsn: 1.439 ± 0.252
3.015SerPro: 3.015 ± 0.471
1.37SerGln: 1.37 ± 0.281
3.152SerArg: 3.152 ± 0.476
3.152SerSer: 3.152 ± 0.575
3.494SerThr: 3.494 ± 0.521
4.385SerVal: 4.385 ± 0.521
0.959SerTrp: 0.959 ± 0.299
0.959SerTyr: 0.959 ± 0.263
0.0SerXaa: 0.0 ± 0.0
Thr
7.4ThrAla: 7.4 ± 0.886
0.343ThrCys: 0.343 ± 0.156
4.728ThrAsp: 4.728 ± 0.646
4.111ThrGlu: 4.111 ± 0.557
1.85ThrPhe: 1.85 ± 0.329
6.029ThrGly: 6.029 ± 0.594
0.822ThrHis: 0.822 ± 0.313
3.289ThrIle: 3.289 ± 0.56
0.959ThrLys: 0.959 ± 0.269
5.618ThrLeu: 5.618 ± 0.517
0.959ThrMet: 0.959 ± 0.263
1.233ThrAsn: 1.233 ± 0.275
4.865ThrPro: 4.865 ± 0.748
1.85ThrGln: 1.85 ± 0.312
4.248ThrArg: 4.248 ± 0.557
3.905ThrSer: 3.905 ± 0.505
5.618ThrThr: 5.618 ± 0.858
6.166ThrVal: 6.166 ± 0.728
1.302ThrTrp: 1.302 ± 0.325
1.37ThrTyr: 1.37 ± 0.455
0.0ThrXaa: 0.0 ± 0.0
Val
10.483ValAla: 10.483 ± 1.225
0.617ValCys: 0.617 ± 0.247
5.07ValAsp: 5.07 ± 0.797
4.18ValGlu: 4.18 ± 0.58
1.987ValPhe: 1.987 ± 0.351
6.372ValGly: 6.372 ± 0.68
0.754ValHis: 0.754 ± 0.273
3.357ValIle: 3.357 ± 0.551
2.878ValLys: 2.878 ± 0.525
5.55ValLeu: 5.55 ± 0.624
1.507ValMet: 1.507 ± 0.312
1.918ValAsn: 1.918 ± 0.392
3.905ValPro: 3.905 ± 0.547
1.85ValGln: 1.85 ± 0.335
5.139ValArg: 5.139 ± 0.649
3.494ValSer: 3.494 ± 0.615
5.618ValThr: 5.618 ± 0.748
5.07ValVal: 5.07 ± 0.681
1.85ValTrp: 1.85 ± 0.348
1.439ValTyr: 1.439 ± 0.341
0.0ValXaa: 0.0 ± 0.0
Trp
1.987TrpAla: 1.987 ± 0.44
0.548TrpCys: 0.548 ± 0.18
1.439TrpAsp: 1.439 ± 0.324
1.302TrpGlu: 1.302 ± 0.338
0.822TrpPhe: 0.822 ± 0.216
1.096TrpGly: 1.096 ± 0.285
0.685TrpHis: 0.685 ± 0.304
1.096TrpIle: 1.096 ± 0.293
0.822TrpLys: 0.822 ± 0.228
1.918TrpLeu: 1.918 ± 0.446
1.028TrpMet: 1.028 ± 0.256
1.028TrpAsn: 1.028 ± 0.338
1.096TrpPro: 1.096 ± 0.244
0.891TrpGln: 0.891 ± 0.22
1.576TrpArg: 1.576 ± 0.317
0.685TrpSer: 0.685 ± 0.272
0.959TrpThr: 0.959 ± 0.267
1.233TrpVal: 1.233 ± 0.273
0.617TrpTrp: 0.617 ± 0.221
0.411TrpTyr: 0.411 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.467TyrAla: 2.467 ± 0.389
0.274TyrCys: 0.274 ± 0.133
1.576TyrAsp: 1.576 ± 0.398
1.37TyrGlu: 1.37 ± 0.373
0.822TyrPhe: 0.822 ± 0.283
1.439TyrGly: 1.439 ± 0.347
0.617TyrHis: 0.617 ± 0.2
0.822TyrIle: 0.822 ± 0.31
0.48TyrLys: 0.48 ± 0.18
1.781TyrLeu: 1.781 ± 0.446
0.206TyrMet: 0.206 ± 0.125
0.411TyrAsn: 0.411 ± 0.147
1.165TyrPro: 1.165 ± 0.301
0.274TyrGln: 0.274 ± 0.15
1.439TyrArg: 1.439 ± 0.296
1.028TyrSer: 1.028 ± 0.276
1.576TyrThr: 1.576 ± 0.326
1.918TyrVal: 1.918 ± 0.32
0.343TyrTrp: 0.343 ± 0.142
0.617TyrTyr: 0.617 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (14596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski