Moraxella phage Mcat13
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PI46|A0A0R6PI46_9CAUD Uncharacterized protein OS=Moraxella phage Mcat13 OX=1647528 PE=4 SV=1
MM1 pKa = 7.39 IASKK5 pKa = 10.57 RR6 pKa = 11.84 IFGLLGEE13 pKa = 4.56 LVNDD17 pKa = 3.65 RR18 pKa = 11.84 VYY20 pKa = 11.12 PLFVPEE26 pKa = 4.74 TADD29 pKa = 3.26 STPPFMVYY37 pKa = 9.4 TLVSNVPDD45 pKa = 3.65 NTLDD49 pKa = 3.51 GATGHH54 pKa = 6.12 EE55 pKa = 4.34 WVGVQIDD62 pKa = 4.68 IYY64 pKa = 11.07 AADD67 pKa = 4.0 YY68 pKa = 11.55 DD69 pKa = 4.13 EE70 pKa = 5.35 TIALAQEE77 pKa = 3.92 AVKK80 pKa = 10.44 RR81 pKa = 11.84 LNTIKK86 pKa = 10.09 PSEE89 pKa = 4.32 YY90 pKa = 9.31 GGVVYY95 pKa = 10.9 VHH97 pKa = 7.72 DD98 pKa = 5.59 DD99 pKa = 3.19 GLYY102 pKa = 9.37 RR103 pKa = 11.84 AIIEE107 pKa = 4.2 YY108 pKa = 9.94 EE109 pKa = 4.13 FWQTIAA115 pKa = 4.44
Molecular weight: 12.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.96
IPC2_protein 4.266
IPC_protein 4.177
Toseland 3.999
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.062
Rodwell 4.012
Grimsley 3.91
Solomon 4.139
Lehninger 4.101
Nozaki 4.266
DTASelect 4.457
Thurlkill 4.037
EMBOSS 4.075
Sillero 4.291
Patrickios 1.977
IPC_peptide 4.139
IPC2_peptide 4.279
IPC2.peptide.svr19 4.18
Protein with the highest isoelectric point:
>tr|A0A0R6PID1|A0A0R6PID1_9CAUD Tail protein OS=Moraxella phage Mcat13 OX=1647528 PE=4 SV=1
MM1 pKa = 7.05 SANVFMMFYY10 pKa = 9.6 VWGWSHH16 pKa = 6.09 EE17 pKa = 4.39 KK18 pKa = 10.24 IAVRR22 pKa = 11.84 YY23 pKa = 9.1 LSAIGYY29 pKa = 8.4 RR30 pKa = 11.84 GKK32 pKa = 10.63 SRR34 pKa = 11.84 ASRR37 pKa = 11.84 HH38 pKa = 5.37 DD39 pKa = 3.4 VRR41 pKa = 11.84 SALIKK46 pKa = 10.73 AEE48 pKa = 4.06 SFLAGLLHH56 pKa = 6.76 KK57 pKa = 10.43 LYY59 pKa = 11.03 SFTT62 pKa = 4.73
Molecular weight: 7.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.26
IPC2_protein 9.911
IPC_protein 10.394
Toseland 10.292
ProMoST 10.043
Dawson 10.496
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.833
Grimsley 10.584
Solomon 10.54
Lehninger 10.511
Nozaki 10.248
DTASelect 10.204
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.409
Patrickios 10.599
IPC_peptide 10.54
IPC2_peptide 8.975
IPC2.peptide.svr19 8.609
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
8423
40
1224
191.4
21.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.118 ± 0.778
0.938 ± 0.19
6.019 ± 0.378
6.328 ± 0.264
3.479 ± 0.171
6.648 ± 0.393
2.101 ± 0.308
6.601 ± 0.343
7.658 ± 0.425
8.607 ± 0.321
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.742 ± 0.238
5.093 ± 0.245
3.336 ± 0.282
4.452 ± 0.221
4.63 ± 0.361
5.829 ± 0.293
5.675 ± 0.234
6.043 ± 0.272
1.27 ± 0.166
3.431 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here