Amino acid dipepetide frequency for Streptomyces phage Scap1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.21AlaAla: 10.21 ± 1.937
0.292AlaCys: 0.292 ± 0.147
5.616AlaAsp: 5.616 ± 0.547
6.709AlaGlu: 6.709 ± 0.656
3.501AlaPhe: 3.501 ± 0.468
7.949AlaGly: 7.949 ± 1.832
1.313AlaHis: 1.313 ± 0.342
5.761AlaIle: 5.761 ± 0.989
7.147AlaLys: 7.147 ± 1.055
8.022AlaLeu: 8.022 ± 1.538
3.501AlaMet: 3.501 ± 0.479
3.209AlaAsn: 3.209 ± 0.45
3.574AlaPro: 3.574 ± 0.468
2.553AlaGln: 2.553 ± 0.632
5.032AlaArg: 5.032 ± 0.57
4.74AlaSer: 4.74 ± 0.805
5.543AlaThr: 5.543 ± 0.616
6.855AlaVal: 6.855 ± 0.708
1.386AlaTrp: 1.386 ± 0.364
2.917AlaTyr: 2.917 ± 0.573
0.0AlaXaa: 0.0 ± 0.0
Cys
0.146CysAla: 0.146 ± 0.126
0.146CysCys: 0.146 ± 0.095
0.438CysAsp: 0.438 ± 0.198
0.365CysGlu: 0.365 ± 0.158
0.146CysPhe: 0.146 ± 0.112
0.219CysGly: 0.219 ± 0.132
0.0CysHis: 0.0 ± 0.0
0.365CysIle: 0.365 ± 0.199
0.146CysLys: 0.146 ± 0.111
0.511CysLeu: 0.511 ± 0.227
0.073CysMet: 0.073 ± 0.085
0.292CysAsn: 0.292 ± 0.156
0.292CysPro: 0.292 ± 0.177
0.0CysGln: 0.0 ± 0.0
0.073CysArg: 0.073 ± 0.07
0.365CysSer: 0.365 ± 0.154
0.073CysThr: 0.073 ± 0.08
0.365CysVal: 0.365 ± 0.158
0.073CysTrp: 0.073 ± 0.06
0.146CysTyr: 0.146 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
7.22AspAla: 7.22 ± 0.614
0.292AspCys: 0.292 ± 0.164
3.282AspAsp: 3.282 ± 0.645
6.199AspGlu: 6.199 ± 0.919
3.136AspPhe: 3.136 ± 0.615
5.907AspGly: 5.907 ± 0.7
0.365AspHis: 0.365 ± 0.14
3.428AspIle: 3.428 ± 0.67
2.844AspLys: 2.844 ± 0.65
5.105AspLeu: 5.105 ± 0.682
1.532AspMet: 1.532 ± 0.281
2.844AspAsn: 2.844 ± 0.481
3.355AspPro: 3.355 ± 0.689
1.896AspGln: 1.896 ± 0.31
3.209AspArg: 3.209 ± 0.673
2.771AspSer: 2.771 ± 0.423
3.209AspThr: 3.209 ± 0.424
3.938AspVal: 3.938 ± 0.615
1.094AspTrp: 1.094 ± 0.334
1.969AspTyr: 1.969 ± 0.416
0.0AspXaa: 0.0 ± 0.0
Glu
5.688GluAla: 5.688 ± 0.709
0.292GluCys: 0.292 ± 0.156
4.011GluAsp: 4.011 ± 0.552
5.543GluGlu: 5.543 ± 0.887
3.209GluPhe: 3.209 ± 0.592
4.084GluGly: 4.084 ± 0.773
1.167GluHis: 1.167 ± 0.377
3.792GluIle: 3.792 ± 0.432
4.011GluLys: 4.011 ± 0.645
5.543GluLeu: 5.543 ± 0.679
1.969GluMet: 1.969 ± 0.255
1.677GluAsn: 1.677 ± 0.474
2.334GluPro: 2.334 ± 0.42
2.553GluGln: 2.553 ± 0.46
3.136GluArg: 3.136 ± 0.467
2.917GluSer: 2.917 ± 0.423
4.23GluThr: 4.23 ± 0.827
6.199GluVal: 6.199 ± 1.03
1.677GluTrp: 1.677 ± 0.328
1.896GluTyr: 1.896 ± 0.421
0.0GluXaa: 0.0 ± 0.0
Phe
3.646PheAla: 3.646 ± 0.604
0.365PheCys: 0.365 ± 0.164
2.844PheAsp: 2.844 ± 0.552
2.625PheGlu: 2.625 ± 0.682
1.532PhePhe: 1.532 ± 0.283
2.844PheGly: 2.844 ± 0.419
0.365PheHis: 0.365 ± 0.169
1.459PheIle: 1.459 ± 0.3
2.625PheLys: 2.625 ± 0.583
2.553PheLeu: 2.553 ± 0.423
1.167PheMet: 1.167 ± 0.318
1.604PheAsn: 1.604 ± 0.359
0.875PhePro: 0.875 ± 0.219
1.24PheGln: 1.24 ± 0.322
2.553PheArg: 2.553 ± 0.414
2.407PheSer: 2.407 ± 0.464
2.99PheThr: 2.99 ± 0.53
2.188PheVal: 2.188 ± 0.423
0.292PheTrp: 0.292 ± 0.13
1.532PheTyr: 1.532 ± 0.397
0.0PheXaa: 0.0 ± 0.0
Gly
7.366GlyAla: 7.366 ± 0.95
0.365GlyCys: 0.365 ± 0.182
4.157GlyAsp: 4.157 ± 0.538
5.105GlyGlu: 5.105 ± 0.732
2.99GlyPhe: 2.99 ± 0.44
6.272GlyGly: 6.272 ± 0.894
1.167GlyHis: 1.167 ± 0.324
4.74GlyIle: 4.74 ± 1.328
3.938GlyLys: 3.938 ± 0.788
7.001GlyLeu: 7.001 ± 1.627
2.261GlyMet: 2.261 ± 0.419
2.115GlyAsn: 2.115 ± 0.599
2.261GlyPro: 2.261 ± 0.401
1.604GlyGln: 1.604 ± 0.308
3.719GlyArg: 3.719 ± 0.431
5.032GlySer: 5.032 ± 0.795
5.907GlyThr: 5.907 ± 0.753
6.345GlyVal: 6.345 ± 0.822
1.823GlyTrp: 1.823 ± 0.489
3.282GlyTyr: 3.282 ± 0.708
0.0GlyXaa: 0.0 ± 0.0
His
1.459HisAla: 1.459 ± 0.414
0.146HisCys: 0.146 ± 0.093
0.729HisAsp: 0.729 ± 0.241
0.948HisGlu: 0.948 ± 0.353
0.365HisPhe: 0.365 ± 0.15
0.875HisGly: 0.875 ± 0.267
0.583HisHis: 0.583 ± 0.199
1.021HisIle: 1.021 ± 0.315
0.511HisLys: 0.511 ± 0.169
1.094HisLeu: 1.094 ± 0.274
0.656HisMet: 0.656 ± 0.199
1.094HisAsn: 1.094 ± 0.297
0.656HisPro: 0.656 ± 0.238
0.802HisGln: 0.802 ± 0.303
0.583HisArg: 0.583 ± 0.209
1.167HisSer: 1.167 ± 0.347
0.656HisThr: 0.656 ± 0.206
1.386HisVal: 1.386 ± 0.35
0.219HisTrp: 0.219 ± 0.176
1.313HisTyr: 1.313 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
5.251IleAla: 5.251 ± 0.826
0.292IleCys: 0.292 ± 0.165
3.865IleAsp: 3.865 ± 0.484
3.646IleGlu: 3.646 ± 0.602
2.115IlePhe: 2.115 ± 0.49
4.23IleGly: 4.23 ± 1.156
0.583IleHis: 0.583 ± 0.291
2.917IleIle: 2.917 ± 0.487
3.428IleLys: 3.428 ± 0.666
4.084IleLeu: 4.084 ± 0.568
1.313IleMet: 1.313 ± 0.343
2.188IleAsn: 2.188 ± 0.337
2.48IlePro: 2.48 ± 0.334
1.75IleGln: 1.75 ± 0.308
2.261IleArg: 2.261 ± 0.554
3.501IleSer: 3.501 ± 0.418
4.157IleThr: 4.157 ± 0.532
3.938IleVal: 3.938 ± 0.514
0.438IleTrp: 0.438 ± 0.158
1.459IleTyr: 1.459 ± 0.322
0.0IleXaa: 0.0 ± 0.0
Lys
5.616LysAla: 5.616 ± 0.836
0.073LysCys: 0.073 ± 0.075
3.209LysAsp: 3.209 ± 0.512
3.428LysGlu: 3.428 ± 0.517
1.969LysPhe: 1.969 ± 0.385
3.792LysGly: 3.792 ± 0.905
1.094LysHis: 1.094 ± 0.395
3.355LysIle: 3.355 ± 0.536
5.032LysLys: 5.032 ± 0.753
5.98LysLeu: 5.98 ± 0.861
1.823LysMet: 1.823 ± 0.486
2.917LysAsn: 2.917 ± 0.577
2.553LysPro: 2.553 ± 0.481
2.844LysGln: 2.844 ± 0.467
2.99LysArg: 2.99 ± 0.545
3.865LysSer: 3.865 ± 0.579
4.157LysThr: 4.157 ± 0.566
3.792LysVal: 3.792 ± 0.55
0.875LysTrp: 0.875 ± 0.269
1.823LysTyr: 1.823 ± 0.289
0.0LysXaa: 0.0 ± 0.0
Leu
9.335LeuAla: 9.335 ± 1.952
0.146LeuCys: 0.146 ± 0.097
5.397LeuAsp: 5.397 ± 0.669
4.74LeuGlu: 4.74 ± 0.651
2.99LeuPhe: 2.99 ± 0.439
6.782LeuGly: 6.782 ± 0.932
1.167LeuHis: 1.167 ± 0.302
4.157LeuIle: 4.157 ± 0.785
5.324LeuLys: 5.324 ± 0.857
4.959LeuLeu: 4.959 ± 0.713
1.823LeuMet: 1.823 ± 0.366
3.428LeuAsn: 3.428 ± 0.535
3.646LeuPro: 3.646 ± 0.49
2.698LeuGln: 2.698 ± 0.68
4.449LeuArg: 4.449 ± 0.583
5.397LeuSer: 5.397 ± 0.899
5.761LeuThr: 5.761 ± 0.738
5.616LeuVal: 5.616 ± 0.652
1.021LeuTrp: 1.021 ± 0.306
2.698LeuTyr: 2.698 ± 0.45
0.0LeuXaa: 0.0 ± 0.0
Met
1.532MetAla: 1.532 ± 0.252
0.146MetCys: 0.146 ± 0.12
2.334MetAsp: 2.334 ± 0.429
1.459MetGlu: 1.459 ± 0.295
0.948MetPhe: 0.948 ± 0.273
1.677MetGly: 1.677 ± 0.272
0.511MetHis: 0.511 ± 0.202
1.386MetIle: 1.386 ± 0.377
1.896MetLys: 1.896 ± 0.423
2.698MetLeu: 2.698 ± 0.599
0.729MetMet: 0.729 ± 0.234
1.24MetAsn: 1.24 ± 0.353
0.875MetPro: 0.875 ± 0.183
0.583MetGln: 0.583 ± 0.195
1.386MetArg: 1.386 ± 0.367
1.969MetSer: 1.969 ± 0.409
1.532MetThr: 1.532 ± 0.289
2.042MetVal: 2.042 ± 0.447
0.292MetTrp: 0.292 ± 0.149
1.021MetTyr: 1.021 ± 0.338
0.0MetXaa: 0.0 ± 0.0
Asn
3.719AsnAla: 3.719 ± 0.44
0.292AsnCys: 0.292 ± 0.143
2.407AsnAsp: 2.407 ± 0.375
2.48AsnGlu: 2.48 ± 0.46
1.313AsnPhe: 1.313 ± 0.256
3.428AsnGly: 3.428 ± 0.583
0.656AsnHis: 0.656 ± 0.218
1.896AsnIle: 1.896 ± 0.299
2.553AsnLys: 2.553 ± 0.397
2.99AsnLeu: 2.99 ± 0.552
0.583AsnMet: 0.583 ± 0.241
1.24AsnAsn: 1.24 ± 0.287
2.48AsnPro: 2.48 ± 0.493
1.532AsnGln: 1.532 ± 0.295
3.209AsnArg: 3.209 ± 0.57
3.063AsnSer: 3.063 ± 0.559
2.48AsnThr: 2.48 ± 0.462
2.625AsnVal: 2.625 ± 0.474
0.656AsnTrp: 0.656 ± 0.22
1.532AsnTyr: 1.532 ± 0.371
0.0AsnXaa: 0.0 ± 0.0
Pro
3.938ProAla: 3.938 ± 0.548
0.438ProCys: 0.438 ± 0.176
3.574ProAsp: 3.574 ± 0.724
2.698ProGlu: 2.698 ± 0.535
1.677ProPhe: 1.677 ± 0.32
2.99ProGly: 2.99 ± 0.488
0.656ProHis: 0.656 ± 0.218
1.896ProIle: 1.896 ± 0.382
2.553ProLys: 2.553 ± 0.375
2.771ProLeu: 2.771 ± 0.464
0.511ProMet: 0.511 ± 0.191
2.407ProAsn: 2.407 ± 0.459
1.969ProPro: 1.969 ± 0.428
1.021ProGln: 1.021 ± 0.271
1.677ProArg: 1.677 ± 0.466
2.48ProSer: 2.48 ± 0.584
3.792ProThr: 3.792 ± 0.603
2.99ProVal: 2.99 ± 0.457
0.511ProTrp: 0.511 ± 0.18
1.386ProTyr: 1.386 ± 0.384
0.0ProXaa: 0.0 ± 0.0
Gln
4.011GlnAla: 4.011 ± 0.698
0.073GlnCys: 0.073 ± 0.06
1.313GlnAsp: 1.313 ± 0.311
0.948GlnGlu: 0.948 ± 0.293
1.459GlnPhe: 1.459 ± 0.295
2.625GlnGly: 2.625 ± 0.499
1.167GlnHis: 1.167 ± 0.306
2.115GlnIle: 2.115 ± 0.454
1.24GlnLys: 1.24 ± 0.234
3.719GlnLeu: 3.719 ± 0.706
1.313GlnMet: 1.313 ± 0.293
1.094GlnAsn: 1.094 ± 0.34
1.021GlnPro: 1.021 ± 0.243
1.24GlnGln: 1.24 ± 0.281
2.188GlnArg: 2.188 ± 0.531
1.094GlnSer: 1.094 ± 0.271
2.334GlnThr: 2.334 ± 0.45
1.896GlnVal: 1.896 ± 0.362
0.219GlnTrp: 0.219 ± 0.135
1.532GlnTyr: 1.532 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
3.792ArgAla: 3.792 ± 0.573
0.292ArgCys: 0.292 ± 0.171
2.844ArgAsp: 2.844 ± 0.514
3.501ArgGlu: 3.501 ± 0.539
1.459ArgPhe: 1.459 ± 0.336
2.48ArgGly: 2.48 ± 0.47
0.802ArgHis: 0.802 ± 0.253
2.698ArgIle: 2.698 ± 0.501
3.501ArgLys: 3.501 ± 0.491
5.324ArgLeu: 5.324 ± 0.809
1.823ArgMet: 1.823 ± 0.29
2.334ArgAsn: 2.334 ± 0.323
2.48ArgPro: 2.48 ± 0.531
2.407ArgGln: 2.407 ± 0.515
3.646ArgArg: 3.646 ± 0.691
2.844ArgSer: 2.844 ± 0.552
3.574ArgThr: 3.574 ± 0.683
4.084ArgVal: 4.084 ± 0.594
0.583ArgTrp: 0.583 ± 0.25
1.604ArgTyr: 1.604 ± 0.412
0.0ArgXaa: 0.0 ± 0.0
Ser
5.251SerAla: 5.251 ± 0.944
0.073SerCys: 0.073 ± 0.076
4.376SerAsp: 4.376 ± 0.584
3.501SerGlu: 3.501 ± 0.431
1.459SerPhe: 1.459 ± 0.323
5.761SerGly: 5.761 ± 0.728
1.677SerHis: 1.677 ± 0.493
2.48SerIle: 2.48 ± 0.323
2.917SerLys: 2.917 ± 0.439
4.303SerLeu: 4.303 ± 0.566
1.313SerMet: 1.313 ± 0.229
2.625SerAsn: 2.625 ± 0.394
2.553SerPro: 2.553 ± 0.474
1.532SerGln: 1.532 ± 0.341
2.261SerArg: 2.261 ± 0.437
3.355SerSer: 3.355 ± 0.8
4.303SerThr: 4.303 ± 0.67
4.522SerVal: 4.522 ± 0.714
1.313SerTrp: 1.313 ± 0.29
2.042SerTyr: 2.042 ± 0.502
0.0SerXaa: 0.0 ± 0.0
Thr
6.782ThrAla: 6.782 ± 1.034
0.219ThrCys: 0.219 ± 0.139
4.303ThrAsp: 4.303 ± 0.744
4.084ThrGlu: 4.084 ± 0.632
2.261ThrPhe: 2.261 ± 0.395
6.199ThrGly: 6.199 ± 0.706
1.021ThrHis: 1.021 ± 0.308
3.792ThrIle: 3.792 ± 0.529
3.938ThrLys: 3.938 ± 0.52
4.886ThrLeu: 4.886 ± 0.641
0.948ThrMet: 0.948 ± 0.262
1.896ThrAsn: 1.896 ± 0.301
4.23ThrPro: 4.23 ± 0.752
2.553ThrGln: 2.553 ± 0.344
3.574ThrArg: 3.574 ± 0.707
3.865ThrSer: 3.865 ± 0.731
4.886ThrThr: 4.886 ± 0.911
5.397ThrVal: 5.397 ± 0.668
0.583ThrTrp: 0.583 ± 0.252
2.407ThrTyr: 2.407 ± 0.509
0.0ThrXaa: 0.0 ± 0.0
Val
6.272ValAla: 6.272 ± 0.553
0.073ValCys: 0.073 ± 0.082
5.032ValAsp: 5.032 ± 0.54
5.47ValGlu: 5.47 ± 0.915
2.698ValPhe: 2.698 ± 0.438
5.616ValGly: 5.616 ± 0.681
1.094ValHis: 1.094 ± 0.27
3.719ValIle: 3.719 ± 0.634
4.084ValLys: 4.084 ± 0.439
6.418ValLeu: 6.418 ± 0.652
1.604ValMet: 1.604 ± 0.328
3.209ValAsn: 3.209 ± 0.572
2.917ValPro: 2.917 ± 0.526
2.188ValGln: 2.188 ± 0.461
4.376ValArg: 4.376 ± 0.631
4.011ValSer: 4.011 ± 0.439
4.959ValThr: 4.959 ± 0.562
4.74ValVal: 4.74 ± 0.735
1.459ValTrp: 1.459 ± 0.37
2.917ValTyr: 2.917 ± 0.595
0.0ValXaa: 0.0 ± 0.0
Trp
0.948TrpAla: 0.948 ± 0.235
0.0TrpCys: 0.0 ± 0.0
1.313TrpAsp: 1.313 ± 0.405
0.802TrpGlu: 0.802 ± 0.236
0.656TrpPhe: 0.656 ± 0.258
1.313TrpGly: 1.313 ± 0.341
0.219TrpHis: 0.219 ± 0.126
1.021TrpIle: 1.021 ± 0.359
0.802TrpLys: 0.802 ± 0.254
1.021TrpLeu: 1.021 ± 0.265
0.292TrpMet: 0.292 ± 0.198
1.532TrpAsn: 1.532 ± 0.405
0.219TrpPro: 0.219 ± 0.111
0.438TrpGln: 0.438 ± 0.15
0.511TrpArg: 0.511 ± 0.238
1.24TrpSer: 1.24 ± 0.429
1.021TrpThr: 1.021 ± 0.326
1.094TrpVal: 1.094 ± 0.33
0.219TrpTrp: 0.219 ± 0.155
0.438TrpTyr: 0.438 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.646TyrAla: 3.646 ± 0.804
0.219TyrCys: 0.219 ± 0.134
2.553TyrAsp: 2.553 ± 0.476
1.677TyrGlu: 1.677 ± 0.287
1.823TyrPhe: 1.823 ± 0.455
2.407TyrGly: 2.407 ± 0.493
0.656TyrHis: 0.656 ± 0.223
1.896TyrIle: 1.896 ± 0.343
2.698TyrLys: 2.698 ± 0.558
2.553TyrLeu: 2.553 ± 0.541
0.948TyrMet: 0.948 ± 0.258
2.188TyrAsn: 2.188 ± 0.382
1.167TyrPro: 1.167 ± 0.317
1.167TyrGln: 1.167 ± 0.259
1.313TyrArg: 1.313 ± 0.326
1.532TyrSer: 1.532 ± 0.369
2.261TyrThr: 2.261 ± 0.457
2.844TyrVal: 2.844 ± 0.497
0.365TyrTrp: 0.365 ± 0.193
1.094TyrTyr: 1.094 ± 0.355
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13713 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski