Streptococcus phage SW4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7W8X5|A0A3S7W8X5_9CAUD Apaf-1 related killer DARK OS=Streptococcus phage SW4 OX=2419654 GN=SW4_047 PE=4 SV=1
MM1 pKa = 7.13VNWVDD6 pKa = 4.58ANGNDD11 pKa = 3.45IPDD14 pKa = 4.08GKK16 pKa = 10.99SEE18 pKa = 4.08DD19 pKa = 4.06FKK21 pKa = 11.35PGMFFSFAGDD31 pKa = 3.4EE32 pKa = 4.36TNITDD37 pKa = 3.65TGEE40 pKa = 3.72GGYY43 pKa = 10.63YY44 pKa = 9.66GGYY47 pKa = 8.9YY48 pKa = 9.56FRR50 pKa = 11.84KK51 pKa = 9.96FEE53 pKa = 4.13FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S5H0T1|A0A3S5H0T1_9CAUD Minor capsid protein OS=Streptococcus phage SW4 OX=2419654 GN=SW4_011 PE=4 SV=1
MM1 pKa = 6.97GTTVSVKK8 pKa = 10.13VDD10 pKa = 2.99LHH12 pKa = 7.19GLEE15 pKa = 4.88KK16 pKa = 10.49KK17 pKa = 10.47CSPEE21 pKa = 3.35AVKK24 pKa = 10.43RR25 pKa = 11.84GKK27 pKa = 10.24VAMIGQMITDD37 pKa = 4.47MEE39 pKa = 4.37PFIPRR44 pKa = 11.84RR45 pKa = 11.84DD46 pKa = 3.71GTLSASGSPFSDD58 pKa = 4.77GIRR61 pKa = 11.84YY62 pKa = 7.2PGPYY66 pKa = 10.0ARR68 pKa = 11.84AQFYY72 pKa = 10.7GSSYY76 pKa = 10.12NKK78 pKa = 9.31NRR80 pKa = 11.84SFVFRR85 pKa = 11.84NYY87 pKa = 7.13TTPGTGKK94 pKa = 9.94RR95 pKa = 11.84WDD97 pKa = 3.53MKK99 pKa = 11.21ASAKK103 pKa = 10.21YY104 pKa = 8.51SKK106 pKa = 10.29QWGEE110 pKa = 3.73VALRR114 pKa = 11.84AMGVKK119 pKa = 10.0

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10560

44

1528

224.7

25.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.358 ± 1.1

0.634 ± 0.131

5.644 ± 0.508

7.121 ± 0.783

4.299 ± 0.242

6.023 ± 0.6

1.345 ± 0.185

7.273 ± 0.417

8.58 ± 0.667

7.727 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.334

5.644 ± 0.327

2.576 ± 0.204

4.621 ± 0.34

3.949 ± 0.462

6.998 ± 0.578

6.25 ± 0.37

5.956 ± 0.268

1.013 ± 0.118

4.053 ± 0.505
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski