Dokdonia sinensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Dokdonia

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3191 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M0G828|A0A3M0G828_9FLAO Uncharacterized protein OS=Dokdonia sinensis OX=2479847 GN=EAX61_06975 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.15KK3 pKa = 10.04YY4 pKa = 10.41IKK6 pKa = 9.77YY7 pKa = 10.57LPVLAIAFAACEE19 pKa = 4.17PEE21 pKa = 3.65FDD23 pKa = 4.97NPIEE27 pKa = 4.09EE28 pKa = 5.41DD29 pKa = 3.5NFFTSGEE36 pKa = 4.13ADD38 pKa = 3.72FSNYY42 pKa = 8.17VALGNSLTAGFADD55 pKa = 4.0GALYY59 pKa = 8.33RR60 pKa = 11.84TAQNDD65 pKa = 3.88SYY67 pKa = 10.46PNIMAQQFALAGGGEE82 pKa = 4.35FTQPLTNDD90 pKa = 3.15NLGGLTLGGNQITANRR106 pKa = 11.84FVLATDD112 pKa = 3.85ANGNPGPAVLAGTPTTDD129 pKa = 2.59ISNVLSGPFNNMGVPGAKK147 pKa = 9.49SFHH150 pKa = 6.51LVTPGYY156 pKa = 10.89GNVANFPAAANPYY169 pKa = 8.83FIRR172 pKa = 11.84IASEE176 pKa = 3.72PNATVLADD184 pKa = 4.18AVAQNPSFFSLWIGNNDD201 pKa = 3.23VLSYY205 pKa = 9.37ATSGGAGTDD214 pKa = 3.14QTGNLDD220 pKa = 3.34PSTYY224 pKa = 10.36GGNDD228 pKa = 2.58ITDD231 pKa = 4.0PNVFASVYY239 pKa = 8.05SQQVAALVGTGAKK252 pKa = 9.73GALVNIADD260 pKa = 3.83VTSIPFFTTVPAQSIPLDD278 pKa = 3.81APTAAFLNSNFALYY292 pKa = 9.37NQQVLPGLVQFGIITAEE309 pKa = 3.74EE310 pKa = 3.6AAARR314 pKa = 11.84QINFAEE320 pKa = 4.73GVNFPTMSDD329 pKa = 3.88DD330 pKa = 4.24DD331 pKa = 4.36LTDD334 pKa = 3.39ISQILQGPPFGLDD347 pKa = 2.72AATAATLGTLRR358 pKa = 11.84QANDD362 pKa = 3.11SDD364 pKa = 5.05LFPLTAAGVLGTLVNDD380 pKa = 3.58NPQLINGVSVPLADD394 pKa = 3.64QFVLTAVEE402 pKa = 4.05QGRR405 pKa = 11.84VATAQASYY413 pKa = 10.87NATIQGLAAANDD425 pKa = 4.03LAYY428 pKa = 10.88VDD430 pKa = 5.78ARR432 pKa = 11.84AQLQQVAGSGVVFDD446 pKa = 5.07GGTLTSTFVTGGAFSLDD463 pKa = 3.32GVHH466 pKa = 6.96PSPRR470 pKa = 11.84GYY472 pKa = 11.29ALTANSIIDD481 pKa = 4.82AINAKK486 pKa = 10.32YY487 pKa = 10.03GATVPKK493 pKa = 10.67VNIGDD498 pKa = 3.77YY499 pKa = 9.74STVNITNNVNN509 pKa = 2.98

Molecular weight: 52.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M0G7T5|A0A3M0G7T5_9FLAO Eco47II family restriction endonuclease OS=Dokdonia sinensis OX=2479847 GN=EAX61_05690 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.92GRR40 pKa = 11.84KK41 pKa = 8.34KK42 pKa = 10.22ISVSSEE48 pKa = 3.61LRR50 pKa = 11.84HH51 pKa = 6.16KK52 pKa = 10.53KK53 pKa = 10.04

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3191

0

3191

1089628

18

5232

341.5

38.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.105 ± 0.039

0.724 ± 0.016

6.197 ± 0.064

6.59 ± 0.039

5.07 ± 0.034

6.801 ± 0.042

1.717 ± 0.022

7.377 ± 0.035

6.739 ± 0.067

9.085 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.022

5.562 ± 0.046

3.545 ± 0.023

3.489 ± 0.028

3.893 ± 0.037

6.176 ± 0.032

6.259 ± 0.065

6.465 ± 0.034

1.042 ± 0.015

3.912 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski