Marinifilaceae bacterium T3-2 S1-C

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinifilaceae; Ancylomarina

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A434ATP8|A0A434ATP8_9BACT DUF4199 domain-containing protein OS=Marinifilaceae bacterium T3-2 S1-C OX=2487017 GN=DLK05_11420 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.22KK3 pKa = 10.3YY4 pKa = 10.85LLLGYY9 pKa = 10.1LLVISTLIVYY19 pKa = 8.81SQTPPTLNAATGATVDD35 pKa = 3.67GNIVITFSDD44 pKa = 3.61PGSYY48 pKa = 8.28RR49 pKa = 11.84TSIQSVSYY57 pKa = 10.68NNTVLPSGALDD68 pKa = 3.57LTVTDD73 pKa = 4.5QIILIPSGDD82 pKa = 3.74PALQVAGSADD92 pKa = 3.99VIVVATGFSDD102 pKa = 3.38ATVSQTIGHH111 pKa = 6.61GAANKK116 pKa = 10.37LGVTTQPVAPSVNGGVFATQPAITVQDD143 pKa = 3.68QYY145 pKa = 12.13GNTCTSDD152 pKa = 3.31GPRR155 pKa = 11.84SITAANGDD163 pKa = 3.95GQSWTLGGTTDD174 pKa = 3.3QDD176 pKa = 3.38ASSGVLTFSGLTATSNVAVSNAYY199 pKa = 8.72LTFSTSGLTSINSNTFSLALNSPPAITASGTATVDD234 pKa = 2.99ADD236 pKa = 3.67FTITFADD243 pKa = 3.49ANGWQSKK250 pKa = 7.37ITSITYY256 pKa = 10.09GGTDD260 pKa = 2.55ISGAYY265 pKa = 7.48NTGGTSGEE273 pKa = 4.05ITFTPSASTALQAAGTADD291 pKa = 3.67FVIVATGFSDD301 pKa = 3.38ATVSQTIGHH310 pKa = 7.0GSPTKK315 pKa = 10.64LGVTTQPVAPSVNGAAFAPQPAIAIQDD342 pKa = 3.56QYY344 pKa = 12.16DD345 pKa = 3.93NVCTTDD351 pKa = 3.09GTRR354 pKa = 11.84TITAAKK360 pKa = 9.8ADD362 pKa = 3.81AGNWTLGGTTDD373 pKa = 3.3QDD375 pKa = 3.38ASSGVLTFSGLTATSS390 pKa = 3.25

Molecular weight: 39.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A434AVJ3|A0A434AVJ3_9BACT Thymidine kinase OS=Marinifilaceae bacterium T3-2 S1-C OX=2487017 GN=tdk PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.89HH16 pKa = 3.93GFRR19 pKa = 11.84EE20 pKa = 4.17RR21 pKa = 11.84MASVSGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.62LSVSSEE47 pKa = 3.87RR48 pKa = 11.84RR49 pKa = 11.84HH50 pKa = 5.76KK51 pKa = 10.9AA52 pKa = 2.86

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3389

0

3389

1141565

22

4052

336.8

38.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.234 ± 0.042

0.979 ± 0.016

5.45 ± 0.034

6.542 ± 0.045

5.118 ± 0.037

6.345 ± 0.04

1.844 ± 0.017

8.274 ± 0.044

7.933 ± 0.051

9.592 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.021

5.872 ± 0.036

3.314 ± 0.02

3.567 ± 0.022

3.837 ± 0.027

6.639 ± 0.038

4.923 ± 0.036

5.977 ± 0.037

1.06 ± 0.017

4.054 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski