Macaca mulatta feces associated virus 2
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5PWS6|A0A1W5PWS6_9VIRU Putative capsid protein OS=Macaca mulatta feces associated virus 2 OX=2499224 PE=4 SV=1
MM1 pKa = 7.01 FVKK4 pKa = 10.65 VSEE7 pKa = 4.5 TYY9 pKa = 10.76 DD10 pKa = 3.38 LSTKK14 pKa = 9.04 IGKK17 pKa = 8.47 MGLVGIHH24 pKa = 5.42 TPEE27 pKa = 3.77 GRR29 pKa = 11.84 LVFKK33 pKa = 10.49 QWEE36 pKa = 4.74 GFFKK40 pKa = 10.52 NYY42 pKa = 9.89 RR43 pKa = 11.84 KK44 pKa = 9.42 IRR46 pKa = 11.84 YY47 pKa = 7.99 HH48 pKa = 6.01 SCDD51 pKa = 2.87 VAMACASMLPADD63 pKa = 4.26 PLQIGVEE70 pKa = 4.06 AGSIAPQDD78 pKa = 3.62 MFNPILYY85 pKa = 9.92 KK86 pKa = 10.44 PVSNDD91 pKa = 3.52 SMSTLLNRR99 pKa = 11.84 LYY101 pKa = 10.96 VGSTAGSPAVAKK113 pKa = 10.87 NSVIDD118 pKa = 3.74 TNDD121 pKa = 2.94 FNFGDD126 pKa = 4.09 SQVSQDD132 pKa = 3.43 ASGADD137 pKa = 3.52 VPVKK141 pKa = 10.67 SVGGDD146 pKa = 3.1 QFQIYY151 pKa = 10.16 YY152 pKa = 10.78 GLLSDD157 pKa = 3.49 TDD159 pKa = 3.73 GWXKK163 pKa = 10.76 AMPQSGLEE171 pKa = 4.07 MKK173 pKa = 10.48 GLYY176 pKa = 9.73 PLVFSTLKK184 pKa = 10.18 TVGQPSNWSSSTRR197 pKa = 11.84 QGIVADD203 pKa = 3.62 TAMNLVGEE211 pKa = 4.47 SQAYY215 pKa = 9.27 QYY217 pKa = 11.05 APNVGFIGQYY227 pKa = 8.92 MRR229 pKa = 11.84 GPAQRR234 pKa = 11.84 MPACDD239 pKa = 3.42 TYY241 pKa = 11.21 LACTGNPNXRR251 pKa = 11.84 VEE253 pKa = 4.59 GEE255 pKa = 4.34 TITIPDD261 pKa = 3.3 SRR263 pKa = 11.84 XNDD266 pKa = 3.53 KK267 pKa = 10.88 AYY269 pKa = 7.35 PTLSKK274 pKa = 9.95 TPTLTRR280 pKa = 11.84 VTGPALSNSMAPNLDD295 pKa = 3.52 MPDD298 pKa = 3.87 CFVGAIVLPPAKK310 pKa = 10.18 LNQLYY315 pKa = 10.26 YY316 pKa = 10.12 RR317 pKa = 11.84 LKK319 pKa = 9.08 VTWTVEE325 pKa = 4.08 FTEE328 pKa = 4.43 PRR330 pKa = 11.84 PLTDD334 pKa = 3.78 LSNWYY339 pKa = 9.71 GIQSIGFQSYY349 pKa = 7.87 ATDD352 pKa = 3.45 YY353 pKa = 9.12 ATQTQALQSSGSSVTLGMLDD373 pKa = 3.59 TANVDD378 pKa = 3.2 AEE380 pKa = 4.48 KK381 pKa = 10.91 IMDD384 pKa = 3.68 GTKK387 pKa = 10.42
Molecular weight: 41.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.966
IPC2_protein 5.156
IPC_protein 5.067
Toseland 4.927
ProMoST 5.219
Dawson 5.054
Bjellqvist 5.194
Wikipedia 4.965
Rodwell 4.94
Grimsley 4.851
Solomon 5.054
Lehninger 5.016
Nozaki 5.169
DTASelect 5.41
Thurlkill 4.952
EMBOSS 4.991
Sillero 5.219
Patrickios 3.656
IPC_peptide 5.054
IPC2_peptide 5.207
IPC2.peptide.svr19 5.251
Protein with the highest isoelectric point:
>tr|A0A1W5PWS6|A0A1W5PWS6_9VIRU Putative capsid protein OS=Macaca mulatta feces associated virus 2 OX=2499224 PE=4 SV=1
MM1 pKa = 7.48 TKK3 pKa = 9.52 TVMITMEE10 pKa = 3.55 RR11 pKa = 11.84 RR12 pKa = 11.84 EE13 pKa = 4.31 TYY15 pKa = 10.48 KK16 pKa = 10.2 EE17 pKa = 4.0 WNWFLFAIRR26 pKa = 11.84 KK27 pKa = 9.31 LDD29 pKa = 3.21 IHH31 pKa = 6.5 KK32 pKa = 10.0 WIIAAEE38 pKa = 4.05 EE39 pKa = 4.3 GKK41 pKa = 10.63 GGYY44 pKa = 8.49 KK45 pKa = 9.74 HH46 pKa = 4.74 WQIRR50 pKa = 11.84 IASRR54 pKa = 11.84 FDD56 pKa = 3.49 AKK58 pKa = 9.14 WWTRR62 pKa = 11.84 ALLIQFGQRR71 pKa = 11.84 SIHH74 pKa = 5.82 TEE76 pKa = 3.63 NASNTWTYY84 pKa = 8.55 EE85 pKa = 3.9 AKK87 pKa = 10.2 EE88 pKa = 4.24 GCYY91 pKa = 9.56 LASWDD96 pKa = 3.92 TLEE99 pKa = 5.01 VRR101 pKa = 11.84 QQRR104 pKa = 11.84 FGKK107 pKa = 9.39 LRR109 pKa = 11.84 KK110 pKa = 7.32 VQKK113 pKa = 10.05 YY114 pKa = 9.74 ALNVLEE120 pKa = 4.42 GTNDD124 pKa = 3.39 RR125 pKa = 11.84 QVMVWVDD132 pKa = 3.31 EE133 pKa = 4.44 EE134 pKa = 4.76 GNSGKK139 pKa = 10.11 SWLIGHH145 pKa = 7.63 LYY147 pKa = 8.26 EE148 pKa = 5.09 TGQAYY153 pKa = 7.68 YY154 pKa = 10.48 APPYY158 pKa = 8.19 LTSVKK163 pKa = 10.38 EE164 pKa = 4.12 IIQTIASLAVKK175 pKa = 10.36 DD176 pKa = 4.29 RR177 pKa = 11.84 DD178 pKa = 4.0 AGNPPKK184 pKa = 10.32 KK185 pKa = 10.04 YY186 pKa = 10.86 ALIDD190 pKa = 3.53 IPRR193 pKa = 11.84 SWKK196 pKa = 9.01 WSNEE200 pKa = 3.36 LYY202 pKa = 10.17 TAIEE206 pKa = 4.52 AIKK209 pKa = 10.37 DD210 pKa = 3.7 GLIMEE215 pKa = 5.1 PRR217 pKa = 11.84 YY218 pKa = 9.79 SAQPINIKK226 pKa = 9.97 GIKK229 pKa = 9.32 VLVVTNTRR237 pKa = 11.84 PKK239 pKa = 10.45 LDD241 pKa = 3.93 KK242 pKa = 10.63 LSKK245 pKa = 10.47 DD246 pKa = 2.67 RR247 pKa = 11.84 WEE249 pKa = 3.85 IFEE252 pKa = 4.4 WDD254 pKa = 3.79 NNPTIDD260 pKa = 3.32 TYY262 pKa = 11.68 LRR264 pKa = 11.84 GAQEE268 pKa = 3.74 KK269 pKa = 8.56 RR270 pKa = 11.84 TLL272 pKa = 3.51
Molecular weight: 31.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.787
IPC2_protein 8.814
IPC_protein 8.726
Toseland 9.414
ProMoST 9.194
Dawson 9.706
Bjellqvist 9.385
Wikipedia 9.882
Rodwell 9.984
Grimsley 9.78
Solomon 9.736
Lehninger 9.692
Nozaki 9.428
DTASelect 9.37
Thurlkill 9.531
EMBOSS 9.853
Sillero 9.619
Patrickios 4.965
IPC_peptide 9.721
IPC2_peptide 7.863
IPC2.peptide.svr19 7.817
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
659
272
387
329.5
36.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.436 ± 0.045
0.91 ± 0.296
5.615 ± 0.455
4.704 ± 1.443
2.883 ± 0.369
7.132 ± 0.881
0.91 ± 0.305
5.918 ± 1.383
6.373 ± 1.335
7.739 ± 0.01
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.035 ± 0.652
4.552 ± 0.077
5.159 ± 1.008
4.704 ± 0.36
4.552 ± 1.125
6.98 ± 1.4
7.436 ± 0.245
5.918 ± 0.821
2.883 ± 1.234
4.704 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here