Staphylococcus virus 108PVL
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0ZS33|A0ZS33_9CAUD dUTP diphosphatase OS=Staphylococcus virus 108PVL OX=360398 GN=dut PE=4 SV=1
MM1 pKa = 7.51 NNRR4 pKa = 11.84 EE5 pKa = 4.1 QIEE8 pKa = 3.98 QSVISASAYY17 pKa = 10.04 NGNDD21 pKa = 3.29 TEE23 pKa = 4.52 GLLKK27 pKa = 10.49 EE28 pKa = 4.44 IEE30 pKa = 4.24 DD31 pKa = 4.28 VYY33 pKa = 11.57 KK34 pKa = 10.64 KK35 pKa = 10.72 AQAFDD40 pKa = 4.66 EE41 pKa = 4.34 ILEE44 pKa = 4.2 GLPNAMQDD52 pKa = 3.61 ALKK55 pKa = 10.63 EE56 pKa = 4.3 DD57 pKa = 3.43 IGLDD61 pKa = 3.48 EE62 pKa = 5.32 AVGIMTGQVVYY73 pKa = 10.25 KK74 pKa = 10.67 YY75 pKa = 10.68 EE76 pKa = 3.97 EE77 pKa = 4.06 EE78 pKa = 4.28 QEE80 pKa = 3.93 NDD82 pKa = 2.92
Molecular weight: 9.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.211
IPC2_protein 3.935
IPC_protein 3.834
Toseland 3.668
ProMoST 3.897
Dawson 3.795
Bjellqvist 4.012
Wikipedia 3.694
Rodwell 3.681
Grimsley 3.579
Solomon 3.783
Lehninger 3.732
Nozaki 3.923
DTASelect 4.037
Thurlkill 3.706
EMBOSS 3.706
Sillero 3.948
Patrickios 3.427
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0ZS47|A0ZS47_9CAUD Uncharacterized protein OS=Staphylococcus virus 108PVL OX=360398 PE=4 SV=1
MM1 pKa = 7.42 GARR4 pKa = 11.84 IEE6 pKa = 4.59 SNNIEE11 pKa = 4.18 QGLKK15 pKa = 10.42 NAVLKK20 pKa = 10.7 MNLNSNVIVKK30 pKa = 10.08 AGAMSLVPLLKK41 pKa = 10.86 SNTPFANTKK50 pKa = 9.27 KK51 pKa = 9.95 HH52 pKa = 5.78 ARR54 pKa = 11.84 DD55 pKa = 3.92 HH56 pKa = 6.19 IAVSNVKK63 pKa = 8.84 TDD65 pKa = 2.81 RR66 pKa = 11.84 HH67 pKa = 4.91 TSEE70 pKa = 4.85 KK71 pKa = 10.29 IVTIGYY77 pKa = 10.15 AKK79 pKa = 10.15 GVSHH83 pKa = 7.61 RR84 pKa = 11.84 IHH86 pKa = 5.72 ATEE89 pKa = 4.86 FGTMYY94 pKa = 10.3 QKK96 pKa = 10.66 PQLFITKK103 pKa = 7.84 TEE105 pKa = 3.97 KK106 pKa = 9.85 QGKK109 pKa = 8.76 NKK111 pKa = 9.58 VLKK114 pKa = 10.18 TMLDD118 pKa = 3.43 TAKK121 pKa = 10.54 RR122 pKa = 11.84 LQKK125 pKa = 10.81
Molecular weight: 13.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.115
IPC2_protein 9.619
IPC_protein 9.575
Toseland 10.701
ProMoST 10.175
Dawson 10.774
Bjellqvist 10.321
Wikipedia 10.862
Rodwell 11.506
Grimsley 10.789
Solomon 10.804
Lehninger 10.789
Nozaki 10.657
DTASelect 10.321
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.672
Patrickios 11.242
IPC_peptide 10.804
IPC2_peptide 8.639
IPC2.peptide.svr19 8.617
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58
1
59
13120
32
1224
222.4
25.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.221 ± 0.345
0.579 ± 0.114
6.181 ± 0.307
7.287 ± 0.52
3.941 ± 0.251
5.8 ± 0.539
1.852 ± 0.161
7.706 ± 0.271
9.611 ± 0.349
7.797 ± 0.281
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.614 ± 0.194
7.066 ± 0.285
2.736 ± 0.169
3.933 ± 0.26
4.306 ± 0.208
6.319 ± 0.302
5.96 ± 0.245
5.716 ± 0.26
1.113 ± 0.18
4.261 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here