Amino acid dipepetide frequency for Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.779AlaAla: 3.779 ± 0.084	0.746AlaCys: 0.746 ± 0.026	2.385AlaAsp: 2.385 ± 0.056	3.314AlaGlu: 3.314 ± 0.067	2.517AlaPhe: 2.517 ± 0.053	3.667AlaGly: 3.667 ± 0.075	0.783AlaHis: 0.783 ± 0.027	5.829AlaIle: 5.829 ± 0.083	4.737AlaLys: 4.737 ± 0.067	5.684AlaLeu: 5.684 ± 0.071	1.618AlaMet: 1.618 ± 0.038	2.579AlaAsn: 2.579 ± 0.049	1.459AlaPro: 1.459 ± 0.039	1.278AlaGln: 1.278 ± 0.036	1.703AlaArg: 1.703 ± 0.038	3.23AlaSer: 3.23 ± 0.055	2.644AlaThr: 2.644 ± 0.051	3.825AlaVal: 3.825 ± 0.068	0.349AlaTrp: 0.349 ± 0.018	2.12AlaTyr: 2.12 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.63CysAla: 0.63 ± 0.026	0.247CysCys: 0.247 ± 0.02	0.684CysAsp: 0.684 ± 0.027	0.843CysGlu: 0.843 ± 0.028	0.499CysPhe: 0.499 ± 0.021	1.077CysGly: 1.077 ± 0.038	0.252CysHis: 0.252 ± 0.013	1.244CysIle: 1.244 ± 0.034	1.108CysLys: 1.108 ± 0.036	0.882CysLeu: 0.882 ± 0.033	0.346CysMet: 0.346 ± 0.018	0.805CysAsn: 0.805 ± 0.028	0.489CysPro: 0.489 ± 0.024	0.226CysGln: 0.226 ± 0.013	0.414CysArg: 0.414 ± 0.023	0.814CysSer: 0.814 ± 0.027	0.641CysThr: 0.641 ± 0.027	0.65CysVal: 0.65 ± 0.027	0.067CysTrp: 0.067 ± 0.008	0.471CysTyr: 0.471 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	2.385AspAla: 2.385 ± 0.057	0.618AspCys: 0.618 ± 0.024	2.213AspAsp: 2.213 ± 0.053	4.109AspGlu: 4.109 ± 0.068	2.753AspPhe: 2.753 ± 0.049	3.051AspGly: 3.051 ± 0.062	0.598AspHis: 0.598 ± 0.026	6.449AspIle: 6.449 ± 0.08	6.059AspLys: 6.059 ± 0.089	4.9AspLeu: 4.9 ± 0.071	1.562AspMet: 1.562 ± 0.04	3.212AspAsn: 3.212 ± 0.055	1.365AspPro: 1.365 ± 0.039	0.803AspGln: 0.803 ± 0.028	1.701AspArg: 1.701 ± 0.04	3.034AspSer: 3.034 ± 0.051	2.486AspThr: 2.486 ± 0.049	3.21AspVal: 3.21 ± 0.061	0.353AspTrp: 0.353 ± 0.018	2.525AspTyr: 2.525 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	4.036GluAla: 4.036 ± 0.063	0.72GluCys: 0.72 ± 0.029	4.648GluAsp: 4.648 ± 0.08	7.539GluGlu: 7.539 ± 0.132	2.98GluPhe: 2.98 ± 0.051	4.183GluGly: 4.183 ± 0.072	0.954GluHis: 0.954 ± 0.034	7.141GluIle: 7.141 ± 0.094	8.644GluLys: 8.644 ± 0.108	6.361GluLeu: 6.361 ± 0.084	1.816GluMet: 1.816 ± 0.048	5.814GluAsn: 5.814 ± 0.08	1.402GluPro: 1.402 ± 0.035	1.679GluGln: 1.679 ± 0.045	2.661GluArg: 2.661 ± 0.067	3.365GluSer: 3.365 ± 0.064	2.799GluThr: 2.799 ± 0.054	4.656GluVal: 4.656 ± 0.076	0.404GluTrp: 0.404 ± 0.021	2.924GluTyr: 2.924 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	2.102PheAla: 2.102 ± 0.052	0.549PheCys: 0.549 ± 0.024	2.177PheAsp: 2.177 ± 0.045	2.495PheGlu: 2.495 ± 0.051	1.99PhePhe: 1.99 ± 0.053	2.729PheGly: 2.729 ± 0.059	0.573PheHis: 0.573 ± 0.024	4.989PheIle: 4.989 ± 0.086	4.122PheLys: 4.122 ± 0.07	4.101PheLeu: 4.101 ± 0.072	1.234PheMet: 1.234 ± 0.04	3.059PheAsn: 3.059 ± 0.066	1.212PhePro: 1.212 ± 0.033	1.03PheGln: 1.03 ± 0.035	1.202PheArg: 1.202 ± 0.035	3.192PheSer: 3.192 ± 0.061	2.229PheThr: 2.229 ± 0.041	2.543PheVal: 2.543 ± 0.051	0.319PheTrp: 0.319 ± 0.017	1.904PheTyr: 1.904 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.054GlyAla: 4.054 ± 0.069	0.883GlyCys: 0.883 ± 0.032	3.151GlyAsp: 3.151 ± 0.058	4.315GlyGlu: 4.315 ± 0.066	2.938GlyPhe: 2.938 ± 0.061	4.217GlyGly: 4.217 ± 0.081	0.965GlyHis: 0.965 ± 0.033	6.801GlyIle: 6.801 ± 0.1	5.882GlyLys: 5.882 ± 0.083	5.09GlyLeu: 5.09 ± 0.078	1.751GlyMet: 1.751 ± 0.046	3.322GlyAsn: 3.322 ± 0.062	1.287GlyPro: 1.287 ± 0.045	1.407GlyGln: 1.407 ± 0.038	2.1GlyArg: 2.1 ± 0.05	3.545GlySer: 3.545 ± 0.07	3.355GlyThr: 3.355 ± 0.067	4.337GlyVal: 4.337 ± 0.065	0.488GlyTrp: 0.488 ± 0.021	2.894GlyTyr: 2.894 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	0.642HisAla: 0.642 ± 0.023	0.225HisCys: 0.225 ± 0.016	0.659HisAsp: 0.659 ± 0.026	0.816HisGlu: 0.816 ± 0.031	0.599HisPhe: 0.599 ± 0.023	0.939HisGly: 0.939 ± 0.032	0.299HisHis: 0.299 ± 0.017	1.477HisIle: 1.477 ± 0.04	1.205HisLys: 1.205 ± 0.035	1.154HisLeu: 1.154 ± 0.035	0.386HisMet: 0.386 ± 0.018	0.879HisAsn: 0.879 ± 0.03	0.567HisPro: 0.567 ± 0.026	0.283HisGln: 0.283 ± 0.02	0.532HisArg: 0.532 ± 0.026	0.839HisSer: 0.839 ± 0.025	0.656HisThr: 0.656 ± 0.026	0.751HisVal: 0.751 ± 0.026	0.11HisTrp: 0.11 ± 0.01	0.574HisTyr: 0.574 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.767IleAla: 5.767 ± 0.085	1.403IleCys: 1.403 ± 0.041	5.845IleAsp: 5.845 ± 0.073	7.492IleGlu: 7.492 ± 0.098	4.416IlePhe: 4.416 ± 0.073	6.376IleGly: 6.376 ± 0.091	1.319IleHis: 1.319 ± 0.033	10.487IleIle: 10.487 ± 0.135	10.307IleLys: 10.307 ± 0.106	9.797IleLeu: 9.797 ± 0.121	2.563IleMet: 2.563 ± 0.05	6.812IleAsn: 6.812 ± 0.107	3.48IlePro: 3.48 ± 0.061	2.183IleGln: 2.183 ± 0.044	3.009IleArg: 3.009 ± 0.057	7.004IleSer: 7.004 ± 0.095	4.916IleThr: 4.916 ± 0.077	6.248IleVal: 6.248 ± 0.086	0.595IleTrp: 0.595 ± 0.022	3.964IleTyr: 3.964 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	5.094LysAla: 5.094 ± 0.07	0.95LysCys: 0.95 ± 0.032	6.866LysAsp: 6.866 ± 0.097	10.454LysGlu: 10.454 ± 0.136	3.535LysPhe: 3.535 ± 0.056	5.658LysGly: 5.658 ± 0.079	1.227LysHis: 1.227 ± 0.036	9.352LysIle: 9.352 ± 0.1	9.504LysLys: 9.504 ± 0.098	7.964LysLeu: 7.964 ± 0.085	2.558LysMet: 2.558 ± 0.054	7.688LysAsn: 7.688 ± 0.103	2.213LysPro: 2.213 ± 0.046	2.219LysGln: 2.219 ± 0.059	3.053LysArg: 3.053 ± 0.057	5.419LysSer: 5.419 ± 0.074	4.113LysThr: 4.113 ± 0.064	6.135LysVal: 6.135 ± 0.085	0.682LysTrp: 0.682 ± 0.027	4.365LysTyr: 4.365 ± 0.078	0.0LysXaa: 0.0 ± 0.0
Leu	5.117LeuAla: 5.117 ± 0.072	1.117LeuCys: 1.117 ± 0.03	5.163LeuAsp: 5.163 ± 0.072	6.276LeuGlu: 6.276 ± 0.095	3.694LeuPhe: 3.694 ± 0.072	6.063LeuGly: 6.063 ± 0.104	1.078LeuHis: 1.078 ± 0.033	8.374LeuIle: 8.374 ± 0.108	9.424LeuLys: 9.424 ± 0.117	7.691LeuLeu: 7.691 ± 0.102	2.346LeuMet: 2.346 ± 0.051	6.21LeuAsn: 6.21 ± 0.092	2.731LeuPro: 2.731 ± 0.061	2.102LeuGln: 2.102 ± 0.056	2.86LeuArg: 2.86 ± 0.057	6.384LeuSer: 6.384 ± 0.082	4.161LeuThr: 4.161 ± 0.074	5.098LeuVal: 5.098 ± 0.076	0.612LeuTrp: 0.612 ± 0.027	3.375LeuTyr: 3.375 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	1.746MetAla: 1.746 ± 0.045	0.306MetCys: 0.306 ± 0.02	1.676MetAsp: 1.676 ± 0.039	2.022MetGlu: 2.022 ± 0.049	1.067MetPhe: 1.067 ± 0.034	1.894MetGly: 1.894 ± 0.046	0.342MetHis: 0.342 ± 0.018	2.297MetIle: 2.297 ± 0.05	2.727MetLys: 2.727 ± 0.047	2.406MetLeu: 2.406 ± 0.048	0.685MetMet: 0.685 ± 0.029	1.682MetAsn: 1.682 ± 0.04	0.918MetPro: 0.918 ± 0.03	0.613MetGln: 0.613 ± 0.025	0.814MetArg: 0.814 ± 0.027	1.584MetSer: 1.584 ± 0.04	1.125MetThr: 1.125 ± 0.033	1.619MetVal: 1.619 ± 0.039	0.163MetTrp: 0.163 ± 0.012	0.92MetTyr: 0.92 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	2.687AsnAla: 2.687 ± 0.055	0.828AsnCys: 0.828 ± 0.027	2.649AsnAsp: 2.649 ± 0.046	4.299AsnGlu: 4.299 ± 0.071	2.871AsnPhe: 2.871 ± 0.058	3.352AsnGly: 3.352 ± 0.067	0.808AsnHis: 0.808 ± 0.026	8.33AsnIle: 8.33 ± 0.119	7.429AsnLys: 7.429 ± 0.108	6.193AsnLeu: 6.193 ± 0.098	1.815AsnMet: 1.815 ± 0.039	5.077AsnAsn: 5.077 ± 0.109	2.097AsnPro: 2.097 ± 0.048	1.209AsnGln: 1.209 ± 0.037	1.922AsnArg: 1.922 ± 0.046	4.018AsnSer: 4.018 ± 0.083	2.989AsnThr: 2.989 ± 0.053	3.674AsnVal: 3.674 ± 0.063	0.478AsnTrp: 0.478 ± 0.021	2.924AsnTyr: 2.924 ± 0.064	0.0AsnXaa: 0.0 ± 0.0
Pro	1.424ProAla: 1.424 ± 0.041	0.349ProCys: 0.349 ± 0.017	1.342ProAsp: 1.342 ± 0.035	2.085ProGlu: 2.085 ± 0.052	1.365ProPhe: 1.365 ± 0.043	1.715ProGly: 1.715 ± 0.05	0.428ProHis: 0.428 ± 0.017	2.947ProIle: 2.947 ± 0.051	2.539ProLys: 2.539 ± 0.052	2.493ProLeu: 2.493 ± 0.055	0.705ProMet: 0.705 ± 0.032	1.641ProAsn: 1.641 ± 0.043	0.603ProPro: 0.603 ± 0.027	0.758ProGln: 0.758 ± 0.026	0.745ProArg: 0.745 ± 0.026	1.756ProSer: 1.756 ± 0.041	1.47ProThr: 1.47 ± 0.038	1.991ProVal: 1.991 ± 0.044	0.244ProTrp: 0.244 ± 0.016	1.367ProTyr: 1.367 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	1.239GlnAla: 1.239 ± 0.035	0.297GlnCys: 0.297 ± 0.017	1.147GlnAsp: 1.147 ± 0.035	1.607GlnGlu: 1.607 ± 0.042	0.865GlnPhe: 0.865 ± 0.03	1.406GlnGly: 1.406 ± 0.037	0.326GlnHis: 0.326 ± 0.019	2.066GlnIle: 2.066 ± 0.045	1.952GlnLys: 1.952 ± 0.04	1.926GlnLeu: 1.926 ± 0.048	0.603GlnMet: 0.603 ± 0.027	1.46GlnAsn: 1.46 ± 0.037	0.531GlnPro: 0.531 ± 0.023	0.71GlnGln: 0.71 ± 0.034	0.872GlnArg: 0.872 ± 0.031	1.311GlnSer: 1.311 ± 0.034	0.952GlnThr: 0.952 ± 0.029	1.369GlnVal: 1.369 ± 0.037	0.223GlnTrp: 0.223 ± 0.015	1.022GlnTyr: 1.022 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	1.709ArgAla: 1.709 ± 0.037	0.417ArgCys: 0.417 ± 0.025	1.716ArgAsp: 1.716 ± 0.041	2.835ArgGlu: 2.835 ± 0.052	1.34ArgPhe: 1.34 ± 0.035	1.914ArgGly: 1.914 ± 0.041	0.461ArgHis: 0.461 ± 0.02	3.079ArgIle: 3.079 ± 0.054	2.993ArgLys: 2.993 ± 0.052	2.684ArgLeu: 2.684 ± 0.044	0.88ArgMet: 0.88 ± 0.031	1.921ArgAsn: 1.921 ± 0.046	0.806ArgPro: 0.806 ± 0.029	0.808ArgGln: 0.808 ± 0.025	1.335ArgArg: 1.335 ± 0.043	1.477ArgSer: 1.477 ± 0.04	1.454ArgThr: 1.454 ± 0.039	2.134ArgVal: 2.134 ± 0.047	0.238ArgTrp: 0.238 ± 0.019	1.383ArgTyr: 1.383 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	2.948SerAla: 2.948 ± 0.063	0.678SerCys: 0.678 ± 0.026	2.683SerAsp: 2.683 ± 0.054	3.702SerGlu: 3.702 ± 0.061	3.124SerPhe: 3.124 ± 0.057	3.87SerGly: 3.87 ± 0.069	0.923SerHis: 0.923 ± 0.031	6.962SerIle: 6.962 ± 0.099	6.28SerLys: 6.28 ± 0.092	5.86SerLeu: 5.86 ± 0.091	1.744SerMet: 1.744 ± 0.042	3.881SerAsn: 3.881 ± 0.072	1.656SerPro: 1.656 ± 0.046	1.428SerGln: 1.428 ± 0.033	1.826SerArg: 1.826 ± 0.043	4.172SerSer: 4.172 ± 0.081	2.996SerThr: 2.996 ± 0.067	3.575SerVal: 3.575 ± 0.059	0.452SerTrp: 0.452 ± 0.025	2.583SerTyr: 2.583 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	2.867ThrAla: 2.867 ± 0.052	0.52ThrCys: 0.52 ± 0.022	2.165ThrAsp: 2.165 ± 0.051	2.858ThrGlu: 2.858 ± 0.062	2.067ThrPhe: 2.067 ± 0.045	3.523ThrGly: 3.523 ± 0.066	0.728ThrHis: 0.728 ± 0.027	4.95ThrIle: 4.95 ± 0.083	3.828ThrLys: 3.828 ± 0.059	4.795ThrLeu: 4.795 ± 0.073	1.14ThrMet: 1.14 ± 0.03	2.67ThrAsn: 2.67 ± 0.058	1.797ThrPro: 1.797 ± 0.046	0.917ThrGln: 0.917 ± 0.026	1.409ThrArg: 1.409 ± 0.038	2.899ThrSer: 2.899 ± 0.053	2.452ThrThr: 2.452 ± 0.052	3.157ThrVal: 3.157 ± 0.054	0.312ThrTrp: 0.312 ± 0.02	1.741ThrTyr: 1.741 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	3.772ValAla: 3.772 ± 0.06	0.845ValCys: 0.845 ± 0.029	3.507ValAsp: 3.507 ± 0.059	4.297ValGlu: 4.297 ± 0.059	2.869ValPhe: 2.869 ± 0.057	4.078ValGly: 4.078 ± 0.065	0.86ValHis: 0.86 ± 0.027	6.005ValIle: 6.005 ± 0.08	5.507ValLys: 5.507 ± 0.087	5.811ValLeu: 5.811 ± 0.071	1.559ValMet: 1.559 ± 0.039	3.505ValAsn: 3.505 ± 0.057	1.928ValPro: 1.928 ± 0.042	1.364ValGln: 1.364 ± 0.039	1.814ValArg: 1.814 ± 0.048	4.107ValSer: 4.107 ± 0.066	3.068ValThr: 3.068 ± 0.058	4.301ValVal: 4.301 ± 0.07	0.378ValTrp: 0.378 ± 0.02	2.357ValTyr: 2.357 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.376TrpAla: 0.376 ± 0.021	0.096TrpCys: 0.096 ± 0.01	0.369TrpAsp: 0.369 ± 0.019	0.454TrpGlu: 0.454 ± 0.025	0.319TrpPhe: 0.319 ± 0.021	0.472TrpGly: 0.472 ± 0.025	0.132TrpHis: 0.132 ± 0.011	0.682TrpIle: 0.682 ± 0.025	0.531TrpLys: 0.531 ± 0.022	0.543TrpLeu: 0.543 ± 0.024	0.198TrpMet: 0.198 ± 0.014	0.482TrpAsn: 0.482 ± 0.021	0.194TrpPro: 0.194 ± 0.013	0.214TrpGln: 0.214 ± 0.013	0.229TrpArg: 0.229 ± 0.016	0.448TrpSer: 0.448 ± 0.026	0.312TrpThr: 0.312 ± 0.018	0.4TrpVal: 0.4 ± 0.023	0.091TrpTrp: 0.091 ± 0.01	0.296TrpTyr: 0.296 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.869TyrAla: 1.869 ± 0.037	0.595TyrCys: 0.595 ± 0.024	2.337TyrAsp: 2.337 ± 0.054	2.915TyrGlu: 2.915 ± 0.052	1.943TyrPhe: 1.943 ± 0.052	2.601TyrGly: 2.601 ± 0.047	0.538TyrHis: 0.538 ± 0.022	4.523TyrIle: 4.523 ± 0.075	4.271TyrLys: 4.271 ± 0.077	3.54TyrLeu: 3.54 ± 0.072	1.096TyrMet: 1.096 ± 0.031	2.953TyrAsn: 2.953 ± 0.056	1.23TyrPro: 1.23 ± 0.033	0.655TyrGln: 0.655 ± 0.028	1.382TyrArg: 1.382 ± 0.038	2.746TyrSer: 2.746 ± 0.051	1.976TyrThr: 1.976 ± 0.053	2.261TyrVal: 2.261 ± 0.049	0.278TyrTrp: 0.278 ± 0.019	1.914TyrTyr: 1.914 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3590 proteins (1052452 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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