Delftia phage PhiW-14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ionavirus; Delftia virus PhiW14

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9DGD3|C9DGD3_9CAUD Uncharacterized protein OS=Delftia phage PhiW-14 OX=665032 GN=162 PE=4 SV=1
MM1 pKa = 7.25LQCILNQVSAEE12 pKa = 3.97ADD14 pKa = 3.1AHH16 pKa = 7.0QGDD19 pKa = 4.71TDD21 pKa = 3.86KK22 pKa = 11.29QHH24 pKa = 6.28YY25 pKa = 8.36PQDD28 pKa = 4.07DD29 pKa = 4.07FHH31 pKa = 8.17VLAYY35 pKa = 10.49RR36 pKa = 11.84FITQEE41 pKa = 3.41HH42 pKa = 6.83DD43 pKa = 3.33LVYY46 pKa = 10.89DD47 pKa = 4.28GSTNGTNDD55 pKa = 3.24GVHH58 pKa = 6.58DD59 pKa = 4.15QDD61 pKa = 5.51DD62 pKa = 4.43VGCEE66 pKa = 3.89SHH68 pKa = 6.66VV69 pKa = 3.31

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9DGH2|C9DGH2_9CAUD DNA ligase OS=Delftia phage PhiW-14 OX=665032 GN=201 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84KK3 pKa = 8.35WLKK6 pKa = 10.78EE7 pKa = 3.57MLEE10 pKa = 4.04TLRR13 pKa = 11.84YY14 pKa = 8.48HH15 pKa = 6.23WQLSHH20 pKa = 6.81CKK22 pKa = 9.8AGRR25 pKa = 11.84HH26 pKa = 4.64RR27 pKa = 11.84MVAVEE32 pKa = 4.18TEE34 pKa = 4.26DD35 pKa = 3.54MVIAPLQKK43 pKa = 10.11NRR45 pKa = 11.84RR46 pKa = 11.84NQQRR50 pKa = 11.84RR51 pKa = 11.84IVGAQVTRR59 pKa = 11.84VCTVCGHH66 pKa = 6.03TEE68 pKa = 3.88TVQEE72 pKa = 4.66FKK74 pKa = 11.44SNTARR79 pKa = 11.84NSWMRR84 pKa = 11.84ATNGLSII91 pKa = 4.58

Molecular weight: 10.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

236

0

236

48902

43

1615

207.2

23.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.19 ± 0.208

1.078 ± 0.067

6.092 ± 0.116

6.386 ± 0.119

3.873 ± 0.115

6.979 ± 0.133

2.413 ± 0.106

5.407 ± 0.09

6.08 ± 0.17

8.161 ± 0.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.268 ± 0.09

4.603 ± 0.102

4.331 ± 0.098

3.998 ± 0.094

5.544 ± 0.114

5.928 ± 0.128

5.671 ± 0.153

6.861 ± 0.141

1.767 ± 0.079

3.372 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski