Thermus phage phi OH2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6B1N1|S6B1N1_9CAUD Uncharacterized protein OS=Thermus phage phi OH2 OX=1352230 PE=4 SV=1
MM1 pKa = 7.66IEE3 pKa = 4.2NPIISDD9 pKa = 3.5RR10 pKa = 11.84HH11 pKa = 6.31PIRR14 pKa = 11.84QGEE17 pKa = 4.06PHH19 pKa = 6.57VIGYY23 pKa = 9.75CEE25 pKa = 4.27GCGGEE30 pKa = 4.2IVEE33 pKa = 4.71GDD35 pKa = 3.76DD36 pKa = 4.11VIEE39 pKa = 4.47FVDD42 pKa = 4.25GLMIHH47 pKa = 6.09QDD49 pKa = 2.7AWCAYY54 pKa = 9.28DD55 pKa = 3.49YY56 pKa = 10.69CAKK59 pKa = 10.46FGQAKK64 pKa = 6.76QTT66 pKa = 3.56

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6C473|S6C473_9CAUD Predicted xylanase/chitin deacetylase OS=Thermus phage phi OH2 OX=1352230 PE=4 SV=1
MM1 pKa = 7.49VSTKK5 pKa = 10.71SPFHH9 pKa = 6.17EE10 pKa = 3.9RR11 pKa = 11.84HH12 pKa = 5.7PIGWRR17 pKa = 11.84FSFEE21 pKa = 4.13VKK23 pKa = 10.41SMYY26 pKa = 10.23KK27 pKa = 10.28LHH29 pKa = 7.45GDD31 pKa = 3.89DD32 pKa = 5.14KK33 pKa = 11.03PKK35 pKa = 10.52KK36 pKa = 9.32SKK38 pKa = 10.62KK39 pKa = 8.81KK40 pKa = 8.32EE41 pKa = 3.61KK42 pKa = 9.79RR43 pKa = 11.84MSMRR47 pKa = 11.84EE48 pKa = 3.74VMEE51 pKa = 3.91LMGIRR56 pKa = 11.84RR57 pKa = 11.84DD58 pKa = 3.2TYY60 pKa = 10.13KK61 pKa = 10.36RR62 pKa = 11.84VRR64 pKa = 11.84GAIRR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 9.85

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11694

44

752

194.9

22.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.739 ± 0.569

0.607 ± 0.122

5.747 ± 0.231

8.355 ± 0.472

4.036 ± 0.219

6.183 ± 0.282

1.839 ± 0.192

6.55 ± 0.212

8.38 ± 0.373

8.252 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.187

4.618 ± 0.224

3.326 ± 0.205

4.13 ± 0.226

5.319 ± 0.352

4.874 ± 0.297

5.541 ± 0.321

6.85 ± 0.265

1.077 ± 0.101

3.908 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski