Lachnospiraceae bacterium CAG:364

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; environmental samples

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2282 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6N8P1|R6N8P1_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium CAG:364 OX=1262983 GN=BN627_01181 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.24FIYY5 pKa = 9.66PAVFSKK11 pKa = 10.01TDD13 pKa = 3.27DD14 pKa = 3.5GSYY17 pKa = 10.31QGYY20 pKa = 8.77FPDD23 pKa = 5.85LEE25 pKa = 4.17CCYY28 pKa = 11.18AKK30 pKa = 10.84GDD32 pKa = 4.03TLEE35 pKa = 5.12DD36 pKa = 3.62AVDD39 pKa = 4.09DD40 pKa = 4.7ANEE43 pKa = 3.75AAYY46 pKa = 10.66NWISLEE52 pKa = 4.25LSEE55 pKa = 5.17EE56 pKa = 4.31DD57 pKa = 3.58CSLPSVSDD65 pKa = 3.28ISDD68 pKa = 3.51MNLKK72 pKa = 10.49EE73 pKa = 3.74GDD75 pKa = 3.23IVRR78 pKa = 11.84NISVNIRR85 pKa = 11.84FYY87 pKa = 11.03EE88 pKa = 4.51GWDD91 pKa = 3.35EE92 pKa = 4.06

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6NYC0|R6NYC0_9FIRM DNA gyrase subunit B OS=Lachnospiraceae bacterium CAG:364 OX=1262983 GN=gyrB PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2282

0

2282

713642

30

3645

312.7

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.093 ± 0.047

1.514 ± 0.024

5.084 ± 0.038

8.422 ± 0.062

4.267 ± 0.04

7.066 ± 0.05

1.662 ± 0.022

7.52 ± 0.053

7.767 ± 0.041

9.104 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.142 ± 0.027

4.367 ± 0.048

3.217 ± 0.025

3.369 ± 0.031

3.896 ± 0.039

5.476 ± 0.036

5.232 ± 0.038

6.817 ± 0.039

0.896 ± 0.022

4.087 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski