Amino acid dipepetide frequency for Lachnospiraceae bacterium CAG:364

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.499AlaAla: 6.499 ± 0.131	1.048AlaCys: 1.048 ± 0.04	3.842AlaAsp: 3.842 ± 0.08	5.371AlaGlu: 5.371 ± 0.098	3.039AlaPhe: 3.039 ± 0.082	5.911AlaGly: 5.911 ± 0.115	1.023AlaHis: 1.023 ± 0.04	4.733AlaIle: 4.733 ± 0.098	5.223AlaLys: 5.223 ± 0.083	6.826AlaLeu: 6.826 ± 0.113	2.346AlaMet: 2.346 ± 0.065	2.458AlaAsn: 2.458 ± 0.067	1.995AlaPro: 1.995 ± 0.052	2.313AlaGln: 2.313 ± 0.06	2.564AlaArg: 2.564 ± 0.06	3.628AlaSer: 3.628 ± 0.079	2.777AlaThr: 2.777 ± 0.072	6.181AlaVal: 6.181 ± 0.104	0.537AlaTrp: 0.537 ± 0.03	2.618AlaTyr: 2.618 ± 0.069	0.001AlaXaa: 0.001 ± 0.001
Cys	0.984CysAla: 0.984 ± 0.043	0.307CysCys: 0.307 ± 0.023	0.793CysAsp: 0.793 ± 0.038	1.033CysGlu: 1.033 ± 0.037	0.687CysPhe: 0.687 ± 0.037	1.457CysGly: 1.457 ± 0.053	0.293CysHis: 0.293 ± 0.022	1.25CysIle: 1.25 ± 0.046	1.008CysLys: 1.008 ± 0.04	1.128CysLeu: 1.128 ± 0.039	0.499CysMet: 0.499 ± 0.029	0.612CysAsn: 0.612 ± 0.032	0.614CysPro: 0.614 ± 0.032	0.464CysGln: 0.464 ± 0.025	0.617CysArg: 0.617 ± 0.033	0.89CysSer: 0.89 ± 0.036	0.779CysThr: 0.779 ± 0.033	1.037CysVal: 1.037 ± 0.042	0.111CysTrp: 0.111 ± 0.012	0.579CysTyr: 0.579 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	3.723AspAla: 3.723 ± 0.076	0.804AspCys: 0.804 ± 0.039	2.218AspAsp: 2.218 ± 0.069	4.498AspGlu: 4.498 ± 0.087	2.756AspPhe: 2.756 ± 0.055	3.825AspGly: 3.825 ± 0.094	0.654AspHis: 0.654 ± 0.03	4.456AspIle: 4.456 ± 0.084	3.767AspLys: 3.767 ± 0.08	4.188AspLeu: 4.188 ± 0.085	1.809AspMet: 1.809 ± 0.046	2.122AspAsn: 2.122 ± 0.06	1.171AspPro: 1.171 ± 0.047	0.879AspGln: 0.879 ± 0.036	1.9AspArg: 1.9 ± 0.058	2.462AspSer: 2.462 ± 0.065	2.969AspThr: 2.969 ± 0.066	3.601AspVal: 3.601 ± 0.075	0.493AspTrp: 0.493 ± 0.022	2.545AspTyr: 2.545 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	5.881GluAla: 5.881 ± 0.103	0.897GluCys: 0.897 ± 0.034	4.56GluAsp: 4.56 ± 0.101	8.908GluGlu: 8.908 ± 0.143	2.983GluPhe: 2.983 ± 0.063	4.75GluGly: 4.75 ± 0.084	1.319GluHis: 1.319 ± 0.047	6.894GluIle: 6.894 ± 0.098	8.849GluLys: 8.849 ± 0.138	7.123GluLeu: 7.123 ± 0.114	2.689GluMet: 2.689 ± 0.065	5.272GluAsn: 5.272 ± 0.092	1.892GluPro: 1.892 ± 0.057	3.028GluGln: 3.028 ± 0.076	3.3GluArg: 3.3 ± 0.068	2.899GluSer: 2.899 ± 0.074	4.106GluThr: 4.106 ± 0.084	4.833GluVal: 4.833 ± 0.095	0.61GluTrp: 0.61 ± 0.029	3.425GluTyr: 3.425 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	2.819PheAla: 2.819 ± 0.071	0.759PheCys: 0.759 ± 0.035	2.381PheAsp: 2.381 ± 0.056	2.836PheGlu: 2.836 ± 0.062	2.131PhePhe: 2.131 ± 0.074	3.101PheGly: 3.101 ± 0.081	0.951PheHis: 0.951 ± 0.038	2.929PheIle: 2.929 ± 0.07	2.123PheLys: 2.123 ± 0.056	4.715PheLeu: 4.715 ± 0.117	1.257PheMet: 1.257 ± 0.044	1.455PheAsn: 1.455 ± 0.046	1.523PhePro: 1.523 ± 0.045	1.67PheGln: 1.67 ± 0.047	1.527PheArg: 1.527 ± 0.044	2.965PheSer: 2.965 ± 0.068	2.342PheThr: 2.342 ± 0.061	2.854PheVal: 2.854 ± 0.064	0.413PheTrp: 0.413 ± 0.026	1.913PheTyr: 1.913 ± 0.056	0.0PheXaa: 0.0 ± 0.0
Gly	5.056GlyAla: 5.056 ± 0.111	1.319GlyCys: 1.319 ± 0.049	3.079GlyAsp: 3.079 ± 0.079	5.087GlyGlu: 5.087 ± 0.098	3.25GlyPhe: 3.25 ± 0.069	4.55GlyGly: 4.55 ± 0.095	1.208GlyHis: 1.208 ± 0.046	6.792GlyIle: 6.792 ± 0.123	6.259GlyLys: 6.259 ± 0.099	5.646GlyLeu: 5.646 ± 0.104	2.479GlyMet: 2.479 ± 0.061	3.247GlyAsn: 3.247 ± 0.075	1.202GlyPro: 1.202 ± 0.045	2.099GlyGln: 2.099 ± 0.051	2.672GlyArg: 2.672 ± 0.069	3.701GlySer: 3.701 ± 0.085	3.935GlyThr: 3.935 ± 0.082	5.026GlyVal: 5.026 ± 0.091	0.747GlyTrp: 0.747 ± 0.056	3.303GlyTyr: 3.303 ± 0.072	0.003GlyXaa: 0.003 ± 0.002
His	1.045HisAla: 1.045 ± 0.037	0.261HisCys: 0.261 ± 0.02	0.799HisAsp: 0.799 ± 0.033	1.057HisGlu: 1.057 ± 0.04	0.789HisPhe: 0.789 ± 0.031	1.177HisGly: 1.177 ± 0.044	0.429HisHis: 0.429 ± 0.037	1.439HisIle: 1.439 ± 0.044	1.073HisLys: 1.073 ± 0.034	1.532HisLeu: 1.532 ± 0.051	0.324HisMet: 0.324 ± 0.023	0.713HisAsn: 0.713 ± 0.033	0.779HisPro: 0.779 ± 0.038	0.544HisGln: 0.544 ± 0.028	0.695HisArg: 0.695 ± 0.033	0.965HisSer: 0.965 ± 0.037	1.037HisThr: 1.037 ± 0.04	1.073HisVal: 1.073 ± 0.045	0.195HisTrp: 0.195 ± 0.022	0.692HisTyr: 0.692 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.448IleAla: 5.448 ± 0.099	1.39IleCys: 1.39 ± 0.056	3.803IleAsp: 3.803 ± 0.089	5.344IleGlu: 5.344 ± 0.097	3.306IlePhe: 3.306 ± 0.078	5.238IleGly: 5.238 ± 0.116	1.386IleHis: 1.386 ± 0.045	5.063IleIle: 5.063 ± 0.098	4.441IleLys: 4.441 ± 0.081	7.795IleLeu: 7.795 ± 0.138	2.106IleMet: 2.106 ± 0.055	2.779IleAsn: 2.779 ± 0.063	3.336IlePro: 3.336 ± 0.07	2.828IleGln: 2.828 ± 0.057	3.198IleArg: 3.198 ± 0.068	5.122IleSer: 5.122 ± 0.092	4.25IleThr: 4.25 ± 0.068	4.883IleVal: 4.883 ± 0.098	0.594IleTrp: 0.594 ± 0.026	2.889IleTyr: 2.889 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	5.458LysAla: 5.458 ± 0.1	0.806LysCys: 0.806 ± 0.038	4.132LysAsp: 4.132 ± 0.084	9.334LysGlu: 9.334 ± 0.12	2.084LysPhe: 2.084 ± 0.057	4.972LysGly: 4.972 ± 0.084	1.017LysHis: 1.017 ± 0.038	5.623LysIle: 5.623 ± 0.091	7.638LysLys: 7.638 ± 0.102	5.804LysLeu: 5.804 ± 0.085	2.563LysMet: 2.563 ± 0.058	4.207LysAsn: 4.207 ± 0.086	2.2LysPro: 2.2 ± 0.067	2.633LysGln: 2.633 ± 0.06	3.383LysArg: 3.383 ± 0.077	3.471LysSer: 3.471 ± 0.07	3.984LysThr: 3.984 ± 0.08	4.675LysVal: 4.675 ± 0.084	0.654LysTrp: 0.654 ± 0.037	3.035LysTyr: 3.035 ± 0.071	0.001LysXaa: 0.001 ± 0.001
Leu	6.537LeuAla: 6.537 ± 0.091	1.572LeuCys: 1.572 ± 0.054	4.806LeuAsp: 4.806 ± 0.077	7.449LeuGlu: 7.449 ± 0.119	4.024LeuPhe: 4.024 ± 0.099	6.208LeuGly: 6.208 ± 0.116	1.487LeuHis: 1.487 ± 0.051	5.884LeuIle: 5.884 ± 0.12	7.096LeuLys: 7.096 ± 0.104	9.212LeuLeu: 9.212 ± 0.158	2.539LeuMet: 2.539 ± 0.072	3.813LeuAsn: 3.813 ± 0.08	3.6LeuPro: 3.6 ± 0.083	3.045LeuGln: 3.045 ± 0.065	3.255LeuArg: 3.255 ± 0.071	6.231LeuSer: 6.231 ± 0.106	4.794LeuThr: 4.794 ± 0.083	5.34LeuVal: 5.34 ± 0.081	0.806LeuTrp: 0.806 ± 0.038	3.338LeuTyr: 3.338 ± 0.078	0.0LeuXaa: 0.0 ± 0.0
Met	2.444MetAla: 2.444 ± 0.064	0.378MetCys: 0.378 ± 0.021	1.672MetAsp: 1.672 ± 0.05	2.931MetGlu: 2.931 ± 0.07	1.051MetPhe: 1.051 ± 0.038	2.299MetGly: 2.299 ± 0.054	0.383MetHis: 0.383 ± 0.026	2.136MetIle: 2.136 ± 0.057	2.867MetLys: 2.867 ± 0.065	2.818MetLeu: 2.818 ± 0.067	1.008MetMet: 1.008 ± 0.04	1.422MetAsn: 1.422 ± 0.048	1.155MetPro: 1.155 ± 0.043	1.113MetGln: 1.113 ± 0.04	1.188MetArg: 1.188 ± 0.04	1.742MetSer: 1.742 ± 0.046	1.704MetThr: 1.704 ± 0.046	1.92MetVal: 1.92 ± 0.056	0.227MetTrp: 0.227 ± 0.017	0.963MetTyr: 0.963 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	2.881AsnAla: 2.881 ± 0.072	0.636AsnCys: 0.636 ± 0.031	1.747AsnAsp: 1.747 ± 0.056	2.881AsnGlu: 2.881 ± 0.068	1.747AsnPhe: 1.747 ± 0.052	3.481AsnGly: 3.481 ± 0.092	0.813AsnHis: 0.813 ± 0.039	3.457AsnIle: 3.457 ± 0.076	2.889AsnLys: 2.889 ± 0.069	4.01AsnLeu: 4.01 ± 0.08	1.449AsnMet: 1.449 ± 0.038	1.738AsnAsn: 1.738 ± 0.064	2.217AsnPro: 2.217 ± 0.058	1.585AsnGln: 1.585 ± 0.05	1.966AsnArg: 1.966 ± 0.06	2.463AsnSer: 2.463 ± 0.067	2.608AsnThr: 2.608 ± 0.062	2.772AsnVal: 2.772 ± 0.059	0.465AsnTrp: 0.465 ± 0.031	1.866AsnTyr: 1.866 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.172ProAla: 2.172 ± 0.061	0.474ProCys: 0.474 ± 0.028	2.009ProAsp: 2.009 ± 0.06	3.226ProGlu: 3.226 ± 0.078	1.63ProPhe: 1.63 ± 0.05	2.056ProGly: 2.056 ± 0.058	0.541ProHis: 0.541 ± 0.029	2.172ProIle: 2.172 ± 0.056	2.278ProLys: 2.278 ± 0.057	2.796ProLeu: 2.796 ± 0.067	0.939ProMet: 0.939 ± 0.038	1.319ProAsn: 1.319 ± 0.041	0.659ProPro: 0.659 ± 0.034	1.129ProGln: 1.129 ± 0.042	0.895ProArg: 0.895 ± 0.034	1.774ProSer: 1.774 ± 0.053	1.501ProThr: 1.501 ± 0.049	2.845ProVal: 2.845 ± 0.062	0.314ProTrp: 0.314 ± 0.024	1.448ProTyr: 1.448 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.304GlnAla: 2.304 ± 0.054	0.378GlnCys: 0.378 ± 0.024	1.604GlnAsp: 1.604 ± 0.049	3.52GlnGlu: 3.52 ± 0.072	1.122GlnPhe: 1.122 ± 0.041	2.326GlnGly: 2.326 ± 0.059	0.425GlnHis: 0.425 ± 0.024	2.682GlnIle: 2.682 ± 0.065	3.184GlnLys: 3.184 ± 0.067	2.833GlnLeu: 2.833 ± 0.067	1.171GlnMet: 1.171 ± 0.036	1.568GlnAsn: 1.568 ± 0.057	0.867GlnPro: 0.867 ± 0.042	1.242GlnGln: 1.242 ± 0.045	1.272GlnArg: 1.272 ± 0.043	1.576GlnSer: 1.576 ± 0.051	1.655GlnThr: 1.655 ± 0.051	2.263GlnVal: 2.263 ± 0.064	0.367GlnTrp: 0.367 ± 0.024	1.334GlnTyr: 1.334 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.311ArgAla: 2.311 ± 0.06	0.537ArgCys: 0.537 ± 0.028	1.743ArgAsp: 1.743 ± 0.056	3.774ArgGlu: 3.774 ± 0.085	1.705ArgPhe: 1.705 ± 0.052	2.274ArgGly: 2.274 ± 0.056	0.67ArgHis: 0.67 ± 0.029	3.241ArgIle: 3.241 ± 0.076	3.549ArgLys: 3.549 ± 0.076	3.328ArgLeu: 3.328 ± 0.072	1.411ArgMet: 1.411 ± 0.042	1.938ArgAsn: 1.938 ± 0.054	1.054ArgPro: 1.054 ± 0.045	1.509ArgGln: 1.509 ± 0.046	1.858ArgArg: 1.858 ± 0.056	1.837ArgSer: 1.837 ± 0.046	1.956ArgThr: 1.956 ± 0.054	2.406ArgVal: 2.406 ± 0.062	0.298ArgTrp: 0.298 ± 0.021	1.555ArgTyr: 1.555 ± 0.045	0.001ArgXaa: 0.001 ± 0.001
Ser	3.647SerAla: 3.647 ± 0.086	0.929SerCys: 0.929 ± 0.043	2.739SerAsp: 2.739 ± 0.06	3.673SerGlu: 3.673 ± 0.068	2.773SerPhe: 2.773 ± 0.064	4.481SerGly: 4.481 ± 0.084	0.961SerHis: 0.961 ± 0.037	4.1SerIle: 4.1 ± 0.082	3.654SerLys: 3.654 ± 0.07	4.841SerLeu: 4.841 ± 0.074	1.904SerMet: 1.904 ± 0.053	2.095SerAsn: 2.095 ± 0.071	1.743SerPro: 1.743 ± 0.051	1.838SerGln: 1.838 ± 0.048	2.299SerArg: 2.299 ± 0.056	3.25SerSer: 3.25 ± 0.089	2.564SerThr: 2.564 ± 0.058	4.408SerVal: 4.408 ± 0.084	0.525SerTrp: 0.525 ± 0.028	2.332SerTyr: 2.332 ± 0.055	0.003SerXaa: 0.003 ± 0.002
Thr	4.284ThrAla: 4.284 ± 0.086	0.646ThrCys: 0.646 ± 0.032	2.826ThrAsp: 2.826 ± 0.073	4.264ThrGlu: 4.264 ± 0.081	2.129ThrPhe: 2.129 ± 0.058	4.672ThrGly: 4.672 ± 0.098	0.876ThrHis: 0.876 ± 0.036	3.687ThrIle: 3.687 ± 0.064	3.446ThrLys: 3.446 ± 0.068	4.797ThrLeu: 4.797 ± 0.088	1.426ThrMet: 1.426 ± 0.05	1.806ThrAsn: 1.806 ± 0.056	2.171ThrPro: 2.171 ± 0.052	1.589ThrGln: 1.589 ± 0.047	1.673ThrArg: 1.673 ± 0.051	2.731ThrSer: 2.731 ± 0.065	2.758ThrThr: 2.758 ± 0.071	4.16ThrVal: 4.16 ± 0.089	0.461ThrTrp: 0.461 ± 0.026	1.921ThrTyr: 1.921 ± 0.054	0.0ThrXaa: 0.0 ± 0.0
Val	4.371ValAla: 4.371 ± 0.091	1.208ValCys: 1.208 ± 0.049	3.381ValAsp: 3.381 ± 0.074	4.996ValGlu: 4.996 ± 0.098	3.135ValPhe: 3.135 ± 0.075	4.295ValGly: 4.295 ± 0.098	1.121ValHis: 1.121 ± 0.044	5.077ValIle: 5.077 ± 0.095	5.004ValLys: 5.004 ± 0.093	6.924ValLeu: 6.924 ± 0.105	2.018ValMet: 2.018 ± 0.059	2.888ValAsn: 2.888 ± 0.059	2.434ValPro: 2.434 ± 0.067	2.214ValGln: 2.214 ± 0.053	2.618ValArg: 2.618 ± 0.062	4.387ValSer: 4.387 ± 0.083	4.005ValThr: 4.005 ± 0.077	4.788ValVal: 4.788 ± 0.098	0.661ValTrp: 0.661 ± 0.031	2.647ValTyr: 2.647 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.503TrpAla: 0.503 ± 0.027	0.165TrpCys: 0.165 ± 0.017	0.51TrpAsp: 0.51 ± 0.029	0.743TrpGlu: 0.743 ± 0.037	0.391TrpPhe: 0.391 ± 0.026	0.688TrpGly: 0.688 ± 0.029	0.163TrpHis: 0.163 ± 0.015	0.727TrpIle: 0.727 ± 0.037	0.768TrpLys: 0.768 ± 0.036	0.81TrpLeu: 0.81 ± 0.036	0.3TrpMet: 0.3 ± 0.02	0.499TrpAsn: 0.499 ± 0.031	0.144TrpPro: 0.144 ± 0.015	0.339TrpGln: 0.339 ± 0.028	0.28TrpArg: 0.28 ± 0.02	0.422TrpSer: 0.422 ± 0.024	0.397TrpThr: 0.397 ± 0.024	0.531TrpVal: 0.531 ± 0.034	0.102TrpTrp: 0.102 ± 0.014	0.479TrpTyr: 0.479 ± 0.049	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.563TyrAla: 2.563 ± 0.058	0.625TyrCys: 0.625 ± 0.027	2.196TyrAsp: 2.196 ± 0.063	3.293TyrGlu: 3.293 ± 0.068	1.934TyrPhe: 1.934 ± 0.057	2.898TyrGly: 2.898 ± 0.065	0.9TyrHis: 0.9 ± 0.037	2.856TyrIle: 2.856 ± 0.067	2.557TyrLys: 2.557 ± 0.068	3.908TyrLeu: 3.908 ± 0.074	1.181TyrMet: 1.181 ± 0.046	1.724TyrAsn: 1.724 ± 0.049	1.418TyrPro: 1.418 ± 0.053	1.634TyrGln: 1.634 ± 0.047	1.795TyrArg: 1.795 ± 0.05	2.246TyrSer: 2.246 ± 0.066	2.201TyrThr: 2.201 ± 0.066	2.573TyrVal: 2.573 ± 0.068	0.38TyrTrp: 0.38 ± 0.03	1.987TyrTyr: 1.987 ± 0.073	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.005

Statistics based on 2282 proteins (713642 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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