Amino acid dipepetide frequency for Bosea sp. Leaf344

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.144AlaAla: 21.144 ± 0.217	1.214AlaCys: 1.214 ± 0.037	6.482AlaAsp: 6.482 ± 0.063	8.371AlaGlu: 8.371 ± 0.093	4.823AlaPhe: 4.823 ± 0.073	12.658AlaGly: 12.658 ± 0.124	2.339AlaHis: 2.339 ± 0.046	7.051AlaIle: 7.051 ± 0.077	3.839AlaLys: 3.839 ± 0.078	16.35AlaLeu: 16.35 ± 0.156	3.856AlaMet: 3.856 ± 0.055	2.589AlaAsn: 2.589 ± 0.058	6.638AlaPro: 6.638 ± 0.11	4.788AlaGln: 4.788 ± 0.067	10.543AlaArg: 10.543 ± 0.114	6.818AlaSer: 6.818 ± 0.088	6.335AlaThr: 6.335 ± 0.081	9.108AlaVal: 9.108 ± 0.102	1.559AlaTrp: 1.559 ± 0.04	2.59AlaTyr: 2.59 ± 0.047	0.001AlaXaa: 0.001 ± 0.001
Cys	1.026CysAla: 1.026 ± 0.026	0.106CysCys: 0.106 ± 0.01	0.526CysAsp: 0.526 ± 0.022	0.457CysGlu: 0.457 ± 0.02	0.319CysPhe: 0.319 ± 0.016	0.938CysGly: 0.938 ± 0.034	0.225CysHis: 0.225 ± 0.014	0.372CysIle: 0.372 ± 0.019	0.156CysLys: 0.156 ± 0.011	0.867CysLeu: 0.867 ± 0.028	0.135CysMet: 0.135 ± 0.011	0.159CysAsn: 0.159 ± 0.012	0.424CysPro: 0.424 ± 0.019	0.203CysGln: 0.203 ± 0.012	0.635CysArg: 0.635 ± 0.026	0.41CysSer: 0.41 ± 0.016	0.368CysThr: 0.368 ± 0.017	0.585CysVal: 0.585 ± 0.025	0.106CysTrp: 0.106 ± 0.01	0.178CysTyr: 0.178 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.769AspAla: 6.769 ± 0.075	0.457AspCys: 0.457 ± 0.019	2.536AspAsp: 2.536 ± 0.065	3.186AspGlu: 3.186 ± 0.059	1.972AspPhe: 1.972 ± 0.041	5.204AspGly: 5.204 ± 0.085	1.123AspHis: 1.123 ± 0.032	2.724AspIle: 2.724 ± 0.052	1.442AspLys: 1.442 ± 0.039	5.82AspLeu: 5.82 ± 0.077	1.089AspMet: 1.089 ± 0.033	0.947AspAsn: 0.947 ± 0.027	3.457AspPro: 3.457 ± 0.051	1.475AspGln: 1.475 ± 0.035	4.252AspArg: 4.252 ± 0.062	2.079AspSer: 2.079 ± 0.041	2.115AspThr: 2.115 ± 0.049	3.66AspVal: 3.66 ± 0.053	0.912AspTrp: 0.912 ± 0.028	1.301AspTyr: 1.301 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.892GluAla: 8.892 ± 0.097	0.3GluCys: 0.3 ± 0.016	2.427GluAsp: 2.427 ± 0.052	2.956GluGlu: 2.956 ± 0.061	1.51GluPhe: 1.51 ± 0.035	4.291GluGly: 4.291 ± 0.061	1.022GluHis: 1.022 ± 0.026	3.583GluIle: 3.583 ± 0.051	1.746GluLys: 1.746 ± 0.044	5.253GluLeu: 5.253 ± 0.074	1.395GluMet: 1.395 ± 0.04	1.187GluAsn: 1.187 ± 0.028	3.148GluPro: 3.148 ± 0.061	2.008GluGln: 2.008 ± 0.043	5.404GluArg: 5.404 ± 0.08	2.233GluSer: 2.233 ± 0.044	3.539GluThr: 3.539 ± 0.054	3.56GluVal: 3.56 ± 0.063	0.587GluTrp: 0.587 ± 0.02	0.727GluTyr: 0.727 ± 0.023	0.002GluXaa: 0.002 ± 0.001
Phe	5.025PheAla: 5.025 ± 0.071	0.368PheCys: 0.368 ± 0.018	2.346PheAsp: 2.346 ± 0.044	1.987PheGlu: 1.987 ± 0.041	1.334PhePhe: 1.334 ± 0.035	3.752PheGly: 3.752 ± 0.057	0.68PheHis: 0.68 ± 0.022	1.568PheIle: 1.568 ± 0.036	0.947PheLys: 0.947 ± 0.03	3.269PheLeu: 3.269 ± 0.058	0.798PheMet: 0.798 ± 0.023	0.873PheAsn: 0.873 ± 0.029	1.621PhePro: 1.621 ± 0.041	0.979PheGln: 0.979 ± 0.027	2.289PheArg: 2.289 ± 0.041	2.028PheSer: 2.028 ± 0.041	1.865PheThr: 1.865 ± 0.043	2.759PheVal: 2.759 ± 0.059	0.538PheTrp: 0.538 ± 0.02	0.831PheTyr: 0.831 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.701GlyAla: 10.701 ± 0.116	0.907GlyCys: 0.907 ± 0.032	4.365GlyAsp: 4.365 ± 0.093	5.253GlyGlu: 5.253 ± 0.074	3.941GlyPhe: 3.941 ± 0.062	7.988GlyGly: 7.988 ± 0.13	1.95GlyHis: 1.95 ± 0.048	4.426GlyIle: 4.426 ± 0.063	2.949GlyLys: 2.949 ± 0.05	10.432GlyLeu: 10.432 ± 0.117	2.284GlyMet: 2.284 ± 0.046	1.839GlyAsn: 1.839 ± 0.056	4.004GlyPro: 4.004 ± 0.062	3.08GlyGln: 3.08 ± 0.057	7.236GlyArg: 7.236 ± 0.087	4.769GlySer: 4.769 ± 0.076	4.278GlyThr: 4.278 ± 0.076	6.196GlyVal: 6.196 ± 0.069	1.405GlyTrp: 1.405 ± 0.037	2.176GlyTyr: 2.176 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.329HisAla: 2.329 ± 0.044	0.218HisCys: 0.218 ± 0.013	1.129HisAsp: 1.129 ± 0.036	0.993HisGlu: 0.993 ± 0.028	0.762HisPhe: 0.762 ± 0.025	1.996HisGly: 1.996 ± 0.047	0.513HisHis: 0.513 ± 0.026	0.804HisIle: 0.804 ± 0.027	0.399HisLys: 0.399 ± 0.018	2.081HisLeu: 2.081 ± 0.041	0.37HisMet: 0.37 ± 0.019	0.385HisAsn: 0.385 ± 0.017	1.277HisPro: 1.277 ± 0.039	0.539HisGln: 0.539 ± 0.02	1.508HisArg: 1.508 ± 0.033	0.811HisSer: 0.811 ± 0.024	0.638HisThr: 0.638 ± 0.026	1.5HisVal: 1.5 ± 0.037	0.331HisTrp: 0.331 ± 0.017	0.483HisTyr: 0.483 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.932IleAla: 7.932 ± 0.08	0.537IleCys: 0.537 ± 0.025	3.286IleAsp: 3.286 ± 0.06	3.269IleGlu: 3.269 ± 0.058	1.563IlePhe: 1.563 ± 0.04	5.491IleGly: 5.491 ± 0.075	0.817IleHis: 0.817 ± 0.027	2.229IleIle: 2.229 ± 0.051	1.281IleLys: 1.281 ± 0.036	4.542IleLeu: 4.542 ± 0.066	0.932IleMet: 0.932 ± 0.031	1.123IleAsn: 1.123 ± 0.034	2.289IlePro: 2.289 ± 0.044	1.143IleGln: 1.143 ± 0.032	3.349IleArg: 3.349 ± 0.046	2.354IleSer: 2.354 ± 0.051	2.462IleThr: 2.462 ± 0.049	4.391IleVal: 4.391 ± 0.057	0.605IleTrp: 0.605 ± 0.022	0.974IleTyr: 0.974 ± 0.031	0.001IleXaa: 0.001 ± 0.001
Lys	4.243LysAla: 4.243 ± 0.07	0.111LysCys: 0.111 ± 0.009	1.377LysAsp: 1.377 ± 0.04	1.279LysGlu: 1.279 ± 0.042	0.714LysPhe: 0.714 ± 0.023	2.532LysGly: 2.532 ± 0.054	0.472LysHis: 0.472 ± 0.02	1.426LysIle: 1.426 ± 0.043	0.904LysLys: 0.904 ± 0.036	3.088LysLeu: 3.088 ± 0.053	0.57LysMet: 0.57 ± 0.027	0.614LysAsn: 0.614 ± 0.028	2.12LysPro: 2.12 ± 0.047	0.916LysGln: 0.916 ± 0.027	2.212LysArg: 2.212 ± 0.05	1.546LysSer: 1.546 ± 0.036	1.653LysThr: 1.653 ± 0.048	2.041LysVal: 2.041 ± 0.05	0.27LysTrp: 0.27 ± 0.017	0.456LysTyr: 0.456 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	16.855LeuAla: 16.855 ± 0.151	0.922LeuCys: 0.922 ± 0.031	6.236LeuAsp: 6.236 ± 0.086	4.98LeuGlu: 4.98 ± 0.084	3.564LeuPhe: 3.564 ± 0.07	9.416LeuGly: 9.416 ± 0.098	1.794LeuHis: 1.794 ± 0.036	5.146LeuIle: 5.146 ± 0.087	3.398LeuLys: 3.398 ± 0.062	10.619LeuLeu: 10.619 ± 0.143	2.401LeuMet: 2.401 ± 0.049	2.276LeuAsn: 2.276 ± 0.041	6.158LeuPro: 6.158 ± 0.079	2.932LeuGln: 2.932 ± 0.052	7.574LeuArg: 7.574 ± 0.083	6.522LeuSer: 6.522 ± 0.081	5.401LeuThr: 5.401 ± 0.071	8.367LeuVal: 8.367 ± 0.088	1.22LeuTrp: 1.22 ± 0.036	1.984LeuTyr: 1.984 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	3.329MetAla: 3.329 ± 0.057	0.136MetCys: 0.136 ± 0.011	0.982MetAsp: 0.982 ± 0.029	0.99MetGlu: 0.99 ± 0.029	0.606MetPhe: 0.606 ± 0.026	1.702MetGly: 1.702 ± 0.04	0.379MetHis: 0.379 ± 0.017	1.283MetIle: 1.283 ± 0.033	0.857MetLys: 0.857 ± 0.027	2.591MetLeu: 2.591 ± 0.043	0.612MetMet: 0.612 ± 0.025	0.659MetAsn: 0.659 ± 0.024	1.525MetPro: 1.525 ± 0.033	0.806MetGln: 0.806 ± 0.022	1.812MetArg: 1.812 ± 0.041	1.542MetSer: 1.542 ± 0.038	1.728MetThr: 1.728 ± 0.043	1.609MetVal: 1.609 ± 0.041	0.174MetTrp: 0.174 ± 0.012	0.231MetTyr: 0.231 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.813AsnAla: 2.813 ± 0.052	0.194AsnCys: 0.194 ± 0.014	1.06AsnAsp: 1.06 ± 0.038	0.995AsnGlu: 0.995 ± 0.03	0.757AsnPhe: 0.757 ± 0.025	2.002AsnGly: 2.002 ± 0.051	0.408AsnHis: 0.408 ± 0.019	1.101AsnIle: 1.101 ± 0.032	0.537AsnLys: 0.537 ± 0.025	2.351AsnLeu: 2.351 ± 0.047	0.447AsnMet: 0.447 ± 0.021	0.509AsnAsn: 0.509 ± 0.022	1.619AsnPro: 1.619 ± 0.04	0.624AsnGln: 0.624 ± 0.023	1.579AsnArg: 1.579 ± 0.035	0.972AsnSer: 0.972 ± 0.035	1.026AsnThr: 1.026 ± 0.03	1.492AsnVal: 1.492 ± 0.04	0.348AsnTrp: 0.348 ± 0.018	0.518AsnTyr: 0.518 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	7.816ProAla: 7.816 ± 0.114	0.335ProCys: 0.335 ± 0.018	3.525ProAsp: 3.525 ± 0.058	3.868ProGlu: 3.868 ± 0.064	2.028ProPhe: 2.028 ± 0.041	5.283ProGly: 5.283 ± 0.064	1.078ProHis: 1.078 ± 0.031	2.32ProIle: 2.32 ± 0.047	1.459ProLys: 1.459 ± 0.038	5.294ProLeu: 5.294 ± 0.073	1.209ProMet: 1.209 ± 0.03	1.169ProAsn: 1.169 ± 0.032	2.928ProPro: 2.928 ± 0.064	1.88ProGln: 1.88 ± 0.043	3.566ProArg: 3.566 ± 0.064	2.635ProSer: 2.635 ± 0.049	2.249ProThr: 2.249 ± 0.042	4.461ProVal: 4.461 ± 0.066	0.672ProTrp: 0.672 ± 0.027	1.083ProTyr: 1.083 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	5.082GlnAla: 5.082 ± 0.077	0.178GlnCys: 0.178 ± 0.012	1.523GlnAsp: 1.523 ± 0.038	1.471GlnGlu: 1.471 ± 0.035	0.959GlnPhe: 0.959 ± 0.03	2.723GlnGly: 2.723 ± 0.046	0.577GlnHis: 0.577 ± 0.022	1.818GlnIle: 1.818 ± 0.037	0.905GlnLys: 0.905 ± 0.03	2.83GlnLeu: 2.83 ± 0.054	0.768GlnMet: 0.768 ± 0.026	0.74GlnAsn: 0.74 ± 0.024	2.026GlnPro: 2.026 ± 0.054	1.185GlnGln: 1.185 ± 0.038	2.741GlnArg: 2.741 ± 0.051	1.7GlnSer: 1.7 ± 0.034	1.516GlnThr: 1.516 ± 0.042	2.053GlnVal: 2.053 ± 0.049	0.319GlnTrp: 0.319 ± 0.016	0.473GlnTyr: 0.473 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	8.952ArgAla: 8.952 ± 0.087	0.522ArgCys: 0.522 ± 0.022	4.288ArgAsp: 4.288 ± 0.06	4.543ArgGlu: 4.543 ± 0.073	3.19ArgPhe: 3.19 ± 0.052	5.285ArgGly: 5.285 ± 0.079	1.796ArgHis: 1.796 ± 0.039	4.341ArgIle: 4.341 ± 0.064	2.115ArgLys: 2.115 ± 0.054	9.139ArgLeu: 9.139 ± 0.109	1.918ArgMet: 1.918 ± 0.036	1.671ArgAsn: 1.671 ± 0.035	4.225ArgPro: 4.225 ± 0.068	2.798ArgGln: 2.798 ± 0.05	6.444ArgArg: 6.444 ± 0.083	3.99ArgSer: 3.99 ± 0.058	3.094ArgThr: 3.094 ± 0.051	4.784ArgVal: 4.784 ± 0.075	1.023ArgTrp: 1.023 ± 0.029	1.695ArgTyr: 1.695 ± 0.04	0.001ArgXaa: 0.001 ± 0.001
Ser	6.283SerAla: 6.283 ± 0.07	0.447SerCys: 0.447 ± 0.019	2.592SerAsp: 2.592 ± 0.052	2.699SerGlu: 2.699 ± 0.052	2.272SerPhe: 2.272 ± 0.048	5.52SerGly: 5.52 ± 0.077	1.024SerHis: 1.024 ± 0.028	2.403SerIle: 2.403 ± 0.049	1.259SerLys: 1.259 ± 0.038	5.761SerLeu: 5.761 ± 0.073	1.147SerMet: 1.147 ± 0.033	1.039SerAsn: 1.039 ± 0.032	2.726SerPro: 2.726 ± 0.051	1.588SerGln: 1.588 ± 0.039	3.771SerArg: 3.771 ± 0.066	2.472SerSer: 2.472 ± 0.05	2.266SerThr: 2.266 ± 0.054	3.787SerVal: 3.787 ± 0.062	0.826SerTrp: 0.826 ± 0.027	1.234SerTyr: 1.234 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	6.124ThrAla: 6.124 ± 0.072	0.343ThrCys: 0.343 ± 0.018	2.268ThrAsp: 2.268 ± 0.047	2.382ThrGlu: 2.382 ± 0.047	1.539ThrPhe: 1.539 ± 0.039	5.069ThrGly: 5.069 ± 0.068	0.916ThrHis: 0.916 ± 0.029	2.816ThrIle: 2.816 ± 0.048	1.134ThrLys: 1.134 ± 0.034	6.028ThrLeu: 6.028 ± 0.086	1.084ThrMet: 1.084 ± 0.028	1.022ThrAsn: 1.022 ± 0.037	3.236ThrPro: 3.236 ± 0.058	1.474ThrGln: 1.474 ± 0.033	3.425ThrArg: 3.425 ± 0.054	2.452ThrSer: 2.452 ± 0.049	2.58ThrThr: 2.58 ± 0.054	3.952ThrVal: 3.952 ± 0.057	0.502ThrTrp: 0.502 ± 0.02	0.972ThrTyr: 0.972 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.056ValAla: 10.056 ± 0.099	0.551ValCys: 0.551 ± 0.022	3.715ValAsp: 3.715 ± 0.059	4.503ValGlu: 4.503 ± 0.065	2.723ValPhe: 2.723 ± 0.048	5.361ValGly: 5.361 ± 0.074	1.258ValHis: 1.258 ± 0.034	3.818ValIle: 3.818 ± 0.06	2.324ValLys: 2.324 ± 0.044	7.566ValLeu: 7.566 ± 0.092	1.912ValMet: 1.912 ± 0.035	1.731ValAsn: 1.731 ± 0.044	3.692ValPro: 3.692 ± 0.056	1.979ValGln: 1.979 ± 0.039	4.482ValArg: 4.482 ± 0.077	4.079ValSer: 4.079 ± 0.07	4.578ValThr: 4.578 ± 0.071	5.689ValVal: 5.689 ± 0.077	0.797ValTrp: 0.797 ± 0.026	1.33ValTyr: 1.33 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.189TrpAla: 1.189 ± 0.028	0.148TrpCys: 0.148 ± 0.013	0.571TrpAsp: 0.571 ± 0.021	0.47TrpGlu: 0.47 ± 0.021	0.515TrpPhe: 0.515 ± 0.022	0.897TrpGly: 0.897 ± 0.032	0.281TrpHis: 0.281 ± 0.016	0.601TrpIle: 0.601 ± 0.024	0.387TrpLys: 0.387 ± 0.018	1.758TrpLeu: 1.758 ± 0.041	0.285TrpMet: 0.285 ± 0.015	0.38TrpAsn: 0.38 ± 0.018	0.701TrpPro: 0.701 ± 0.026	0.564TrpGln: 0.564 ± 0.021	1.255TrpArg: 1.255 ± 0.034	0.818TrpSer: 0.818 ± 0.029	0.739TrpThr: 0.739 ± 0.025	0.684TrpVal: 0.684 ± 0.023	0.221TrpTrp: 0.221 ± 0.016	0.263TrpTyr: 0.263 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.536TyrAla: 2.536 ± 0.046	0.202TyrCys: 0.202 ± 0.013	1.287TyrAsp: 1.287 ± 0.029	1.117TyrGlu: 1.117 ± 0.029	0.766TyrPhe: 0.766 ± 0.027	2.071TyrGly: 2.071 ± 0.046	0.414TyrHis: 0.414 ± 0.017	0.737TyrIle: 0.737 ± 0.024	0.506TyrLys: 0.506 ± 0.022	2.127TyrLeu: 2.127 ± 0.045	0.338TyrMet: 0.338 ± 0.016	0.439TyrAsn: 0.439 ± 0.019	1.046TyrPro: 1.046 ± 0.029	0.629TyrGln: 0.629 ± 0.021	1.735TyrArg: 1.735 ± 0.038	0.884TyrSer: 0.884 ± 0.03	0.889TyrThr: 0.889 ± 0.029	1.465TyrVal: 1.465 ± 0.034	0.312TyrTrp: 0.312 ± 0.017	0.457TyrTyr: 0.457 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 3876 proteins (1240282 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---