Zahedan rhabdovirus
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6CHI1|A0A0R6CHI1_9RHAB Putative matrix protein OS=Zahedan rhabdovirus OX=1620507 GN=M PE=4 SV=1
MM1 pKa = 7.62 SKK3 pKa = 10.34 RR4 pKa = 11.84 FRR6 pKa = 11.84 VPSYY10 pKa = 10.29 DD11 pKa = 3.39 PSRR14 pKa = 11.84 MVEE17 pKa = 3.99 VAKK20 pKa = 10.72 AADD23 pKa = 3.29 NAYY26 pKa = 9.79 EE27 pKa = 4.13 NEE29 pKa = 4.0 VEE31 pKa = 4.19 KK32 pKa = 11.32 DD33 pKa = 3.04 KK34 pKa = 11.14 DD35 pKa = 3.75 IPPLTQALSAITLTRR50 pKa = 11.84 EE51 pKa = 3.52 EE52 pKa = 4.28 EE53 pKa = 3.98 ARR55 pKa = 11.84 LGDD58 pKa = 4.32 DD59 pKa = 4.59 SYY61 pKa = 12.1 LSDD64 pKa = 4.09 SDD66 pKa = 5.09 LDD68 pKa = 4.62 RR69 pKa = 11.84 SDD71 pKa = 5.07 KK72 pKa = 11.28 DD73 pKa = 4.0 DD74 pKa = 5.25 DD75 pKa = 4.18 EE76 pKa = 4.82 STPSSCSSHH85 pKa = 6.73 RR86 pKa = 11.84 FADD89 pKa = 3.76 SEE91 pKa = 4.3 GAKK94 pKa = 10.03 AQFYY98 pKa = 10.84 RR99 pKa = 11.84 KK100 pKa = 9.42 INFDD104 pKa = 3.37 RR105 pKa = 11.84 GIEE108 pKa = 3.85 YY109 pKa = 9.81 HH110 pKa = 6.85 IPEE113 pKa = 5.69 KK114 pKa = 10.24 ITTPQDD120 pKa = 3.94 AIDD123 pKa = 4.7 DD124 pKa = 4.24 LLSYY128 pKa = 9.83 MEE130 pKa = 5.07 DD131 pKa = 2.74 MGMIDD136 pKa = 3.91 SYY138 pKa = 11.48 RR139 pKa = 11.84 IDD141 pKa = 3.25 FRR143 pKa = 11.84 NRR145 pKa = 11.84 RR146 pKa = 11.84 KK147 pKa = 10.29 VSINPRR153 pKa = 11.84 STIPVRR159 pKa = 11.84 DD160 pKa = 4.29 EE161 pKa = 3.67 IPEE164 pKa = 3.99 EE165 pKa = 4.49 RR166 pKa = 11.84 FTPPPIKK173 pKa = 10.04 MIVQDD178 pKa = 4.29 PPKK181 pKa = 10.46 ADD183 pKa = 3.04 TGAIAKK189 pKa = 7.49 STPKK193 pKa = 9.44 STPKK197 pKa = 9.97 KK198 pKa = 8.98 VKK200 pKa = 9.72 AKK202 pKa = 10.1 KK203 pKa = 10.18 VSLPSSGKK211 pKa = 10.19 LPVYY215 pKa = 10.2 QYY217 pKa = 9.54 ITIYY221 pKa = 10.44 GKK223 pKa = 10.79 KK224 pKa = 10.13 LDD226 pKa = 3.88 IDD228 pKa = 4.21 LNLYY232 pKa = 10.58 CGGCDD237 pKa = 4.43 LTDD240 pKa = 4.24 FDD242 pKa = 6.31 DD243 pKa = 3.93 KK244 pKa = 11.52 KK245 pKa = 10.21 KK246 pKa = 9.58 WYY248 pKa = 8.78 YY249 pKa = 10.44 HH250 pKa = 7.07 AICSHH255 pKa = 6.77 KK256 pKa = 10.46 IKK258 pKa = 10.49 GIKK261 pKa = 9.62 LISIDD266 pKa = 3.73 MNN268 pKa = 3.85
Molecular weight: 30.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.64
IPC2_protein 5.703
IPC_protein 5.69
Toseland 5.906
ProMoST 5.97
Dawson 5.868
Bjellqvist 5.906
Wikipedia 5.855
Rodwell 5.817
Grimsley 5.995
Solomon 5.868
Lehninger 5.855
Nozaki 6.084
DTASelect 6.287
Thurlkill 6.186
EMBOSS 6.173
Sillero 6.173
Patrickios 4.329
IPC_peptide 5.881
IPC2_peptide 6.122
IPC2.peptide.svr19 6.281
Protein with the highest isoelectric point:
>tr|A0A0R6CHI1|A0A0R6CHI1_9RHAB Putative matrix protein OS=Zahedan rhabdovirus OX=1620507 GN=M PE=4 SV=1
MM1 pKa = 7.55 NVITWTKK8 pKa = 10.42 GKK10 pKa = 10.32 VSQAISKK17 pKa = 10.15 RR18 pKa = 11.84 GKK20 pKa = 10.41 KK21 pKa = 9.78 EE22 pKa = 4.21 DD23 pKa = 3.84 GDD25 pKa = 4.52 DD26 pKa = 3.7 GAKK29 pKa = 9.91 IPSYY33 pKa = 10.94 DD34 pKa = 4.12 LTPSAPPDD42 pKa = 3.58 DD43 pKa = 4.96 GYY45 pKa = 11.24 QYY47 pKa = 10.22 RR48 pKa = 11.84 TINVEE53 pKa = 3.82 AEE55 pKa = 4.07 VDD57 pKa = 3.7 VSVCLDD63 pKa = 3.2 KK64 pKa = 11.54 FEE66 pKa = 4.67 TRR68 pKa = 11.84 MEE70 pKa = 4.86 AIVAPLINLHH80 pKa = 6.09 MDD82 pKa = 3.28 YY83 pKa = 10.56 KK84 pKa = 11.44 GEE86 pKa = 3.87 GRR88 pKa = 11.84 YY89 pKa = 10.2 KK90 pKa = 10.61 NIWLPLVMVAVFGLKK105 pKa = 10.29 LGQRR109 pKa = 11.84 SRR111 pKa = 11.84 DD112 pKa = 3.35 NTVYY116 pKa = 10.57 KK117 pKa = 10.67 VQFGKK122 pKa = 10.73 GLKK125 pKa = 10.23 LKK127 pKa = 10.2 IKK129 pKa = 9.69 SRR131 pKa = 11.84 RR132 pKa = 11.84 PFEE135 pKa = 4.01 MDD137 pKa = 4.88 RR138 pKa = 11.84 KK139 pKa = 9.97 ISWNQFQKK147 pKa = 10.8 YY148 pKa = 8.8 LCNGLTSEE156 pKa = 4.03 WSIRR160 pKa = 11.84 CQLEE164 pKa = 4.07 YY165 pKa = 10.96 TLVDD169 pKa = 3.26 YY170 pKa = 11.52 SMKK173 pKa = 10.69 HH174 pKa = 4.35 VDD176 pKa = 5.19 KK177 pKa = 11.1 EE178 pKa = 3.87 LLQYY182 pKa = 10.26 LRR184 pKa = 11.84 AMRR187 pKa = 11.84 VRR189 pKa = 11.84 FEE191 pKa = 3.86 VDD193 pKa = 2.71 EE194 pKa = 4.27 NQNLVLIMDD203 pKa = 4.49 YY204 pKa = 11.46
Molecular weight: 23.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.283
IPC2_protein 8.258
IPC_protein 8.214
Toseland 8.697
ProMoST 8.77
Dawson 9.048
Bjellqvist 8.975
Wikipedia 9.18
Rodwell 9.18
Grimsley 8.99
Solomon 9.18
Lehninger 9.151
Nozaki 9.048
DTASelect 8.858
Thurlkill 8.96
EMBOSS 9.194
Sillero 9.151
Patrickios 4.52
IPC_peptide 9.18
IPC2_peptide 7.644
IPC2.peptide.svr19 7.644
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3603
204
2170
720.6
82.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.079 ± 0.785
1.665 ± 0.446
6.384 ± 0.705
6.495 ± 0.236
4.191 ± 0.282
5.828 ± 0.28
2.609 ± 0.382
6.245 ± 0.282
5.773 ± 0.74
9.77 ± 0.987
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.391
4.33 ± 0.314
4.746 ± 0.495
3.053 ± 0.324
6.328 ± 0.349
6.273 ± 0.578
6.245 ± 0.332
6.273 ± 0.433
1.971 ± 0.341
4.219 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here