Eupatorium yellow vein betasatellite 
Average proteome isoelectric point is 4.54 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|Q8AYW8|Q8AYW8_9VIRU Putative C1 protein OS=Eupatorium yellow vein betasatellite OX=221054 GN=C1 PE=4 SV=1 
MM1 pKa = 7.72  TITYY5 pKa = 10.55  NNGWGLKK12 pKa = 9.87  FIIDD16 pKa = 3.62  VRR18 pKa = 11.84  LQPTLRR24 pKa = 11.84  VMVKK28 pKa = 9.9  VFSTNQPVMSCYY40 pKa = 10.79  NCTIPYY46 pKa = 8.77  TYY48 pKa = 11.14  VEE50 pKa = 4.29  IQPPFDD56 pKa = 4.05  FNGTEE61 pKa = 3.84  EE62 pKa = 4.56  AIKK65 pKa = 10.86  NRR67 pKa = 11.84  LEE69 pKa = 3.8  VMYY72 pKa = 10.39  RR73 pKa = 11.84  EE74 pKa = 4.32  SNIFDD79 pKa = 3.89  FKK81 pKa = 11.0  EE82 pKa = 3.9  EE83 pKa = 4.03  EE84 pKa = 4.09  MLDD87 pKa = 3.98  TIDD90 pKa = 6.45  DD91 pKa = 4.06  LMLDD95 pKa = 4.17  TFNHH99 pKa = 6.16  IGIDD103 pKa = 3.72  TLGHH107 pKa = 5.6  CFIRR111 pKa = 11.84  CKK113 pKa = 10.66  YY114 pKa = 10.24  AVV116 pKa = 3.22   
 Molecular weight: 13.64 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.541 
IPC2_protein 4.774 
IPC_protein 4.635 
Toseland    4.495 
ProMoST     4.762 
Dawson      4.596 
Bjellqvist  4.736 
Wikipedia   4.482 
Rodwell     4.482 
Grimsley    4.406 
Solomon     4.584 
Lehninger   4.546 
Nozaki      4.711 
DTASelect   4.876 
Thurlkill   4.507 
EMBOSS      4.495 
Sillero     4.762 
Patrickios  3.007 
IPC_peptide 4.596 
IPC2_peptide  4.749 
IPC2.peptide.svr19  4.739 
 Protein with the highest isoelectric point: 
>tr|Q8AYW8|Q8AYW8_9VIRU Putative C1 protein OS=Eupatorium yellow vein betasatellite OX=221054 GN=C1 PE=4 SV=1 
MM1 pKa = 7.72  TITYY5 pKa = 10.55  NNGWGLKK12 pKa = 9.87  FIIDD16 pKa = 3.62  VRR18 pKa = 11.84  LQPTLRR24 pKa = 11.84  VMVKK28 pKa = 9.9  VFSTNQPVMSCYY40 pKa = 10.79  NCTIPYY46 pKa = 8.77  TYY48 pKa = 11.14  VEE50 pKa = 4.29  IQPPFDD56 pKa = 4.05  FNGTEE61 pKa = 3.84  EE62 pKa = 4.56  AIKK65 pKa = 10.86  NRR67 pKa = 11.84  LEE69 pKa = 3.8  VMYY72 pKa = 10.39  RR73 pKa = 11.84  EE74 pKa = 4.32  SNIFDD79 pKa = 3.89  FKK81 pKa = 11.0  EE82 pKa = 3.9  EE83 pKa = 4.03  EE84 pKa = 4.09  MLDD87 pKa = 3.98  TIDD90 pKa = 6.45  DD91 pKa = 4.06  LMLDD95 pKa = 4.17  TFNHH99 pKa = 6.16  IGIDD103 pKa = 3.72  TLGHH107 pKa = 5.6  CFIRR111 pKa = 11.84  CKK113 pKa = 10.66  YY114 pKa = 10.24  AVV116 pKa = 3.22   
 Molecular weight: 13.64 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.541 
IPC2_protein 4.774 
IPC_protein 4.635 
Toseland    4.495 
ProMoST     4.762 
Dawson      4.596 
Bjellqvist  4.736 
Wikipedia   4.482 
Rodwell     4.482 
Grimsley    4.406 
Solomon     4.584 
Lehninger   4.546 
Nozaki      4.711 
DTASelect   4.876 
Thurlkill   4.507 
EMBOSS      4.495 
Sillero     4.762 
Patrickios  3.007 
IPC_peptide 4.596 
IPC2_peptide  4.749 
IPC2.peptide.svr19  4.739 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        1 
 
        
        0
 
        
        1 
         
        116
 
        116
 
        116
 
        116.0
 
        13.64
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        1.724 ± 0.0
3.448 ± 0.0
 
        6.897 ± 0.0
6.897 ± 0.0
 
        6.897 ± 0.0
4.31 ± 0.0
 
        1.724 ± 0.0
9.483 ± 0.0
       
        4.31 ± 0.0
6.897 ± 0.0
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        5.172 ± 0.0
6.897 ± 0.0
 
        4.31 ± 0.0
2.586 ± 0.0
 
        4.31 ± 0.0
2.586 ± 0.0
 
        8.621 ± 0.0
6.897 ± 0.0
       
        0.862 ± 0.0
5.172 ± 0.0
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here