Eupatorium yellow vein betasatellite
Average proteome isoelectric point is 4.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8AYW8|Q8AYW8_9VIRU Putative C1 protein OS=Eupatorium yellow vein betasatellite OX=221054 GN=C1 PE=4 SV=1
MM1 pKa = 7.72 TITYY5 pKa = 10.55 NNGWGLKK12 pKa = 9.87 FIIDD16 pKa = 3.62 VRR18 pKa = 11.84 LQPTLRR24 pKa = 11.84 VMVKK28 pKa = 9.9 VFSTNQPVMSCYY40 pKa = 10.79 NCTIPYY46 pKa = 8.77 TYY48 pKa = 11.14 VEE50 pKa = 4.29 IQPPFDD56 pKa = 4.05 FNGTEE61 pKa = 3.84 EE62 pKa = 4.56 AIKK65 pKa = 10.86 NRR67 pKa = 11.84 LEE69 pKa = 3.8 VMYY72 pKa = 10.39 RR73 pKa = 11.84 EE74 pKa = 4.32 SNIFDD79 pKa = 3.89 FKK81 pKa = 11.0 EE82 pKa = 3.9 EE83 pKa = 4.03 EE84 pKa = 4.09 MLDD87 pKa = 3.98 TIDD90 pKa = 6.45 DD91 pKa = 4.06 LMLDD95 pKa = 4.17 TFNHH99 pKa = 6.16 IGIDD103 pKa = 3.72 TLGHH107 pKa = 5.6 CFIRR111 pKa = 11.84 CKK113 pKa = 10.66 YY114 pKa = 10.24 AVV116 pKa = 3.22
Molecular weight: 13.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.541
IPC2_protein 4.774
IPC_protein 4.635
Toseland 4.495
ProMoST 4.762
Dawson 4.596
Bjellqvist 4.736
Wikipedia 4.482
Rodwell 4.482
Grimsley 4.406
Solomon 4.584
Lehninger 4.546
Nozaki 4.711
DTASelect 4.876
Thurlkill 4.507
EMBOSS 4.495
Sillero 4.762
Patrickios 3.007
IPC_peptide 4.596
IPC2_peptide 4.749
IPC2.peptide.svr19 4.739
Protein with the highest isoelectric point:
>tr|Q8AYW8|Q8AYW8_9VIRU Putative C1 protein OS=Eupatorium yellow vein betasatellite OX=221054 GN=C1 PE=4 SV=1
MM1 pKa = 7.72 TITYY5 pKa = 10.55 NNGWGLKK12 pKa = 9.87 FIIDD16 pKa = 3.62 VRR18 pKa = 11.84 LQPTLRR24 pKa = 11.84 VMVKK28 pKa = 9.9 VFSTNQPVMSCYY40 pKa = 10.79 NCTIPYY46 pKa = 8.77 TYY48 pKa = 11.14 VEE50 pKa = 4.29 IQPPFDD56 pKa = 4.05 FNGTEE61 pKa = 3.84 EE62 pKa = 4.56 AIKK65 pKa = 10.86 NRR67 pKa = 11.84 LEE69 pKa = 3.8 VMYY72 pKa = 10.39 RR73 pKa = 11.84 EE74 pKa = 4.32 SNIFDD79 pKa = 3.89 FKK81 pKa = 11.0 EE82 pKa = 3.9 EE83 pKa = 4.03 EE84 pKa = 4.09 MLDD87 pKa = 3.98 TIDD90 pKa = 6.45 DD91 pKa = 4.06 LMLDD95 pKa = 4.17 TFNHH99 pKa = 6.16 IGIDD103 pKa = 3.72 TLGHH107 pKa = 5.6 CFIRR111 pKa = 11.84 CKK113 pKa = 10.66 YY114 pKa = 10.24 AVV116 pKa = 3.22
Molecular weight: 13.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.541
IPC2_protein 4.774
IPC_protein 4.635
Toseland 4.495
ProMoST 4.762
Dawson 4.596
Bjellqvist 4.736
Wikipedia 4.482
Rodwell 4.482
Grimsley 4.406
Solomon 4.584
Lehninger 4.546
Nozaki 4.711
DTASelect 4.876
Thurlkill 4.507
EMBOSS 4.495
Sillero 4.762
Patrickios 3.007
IPC_peptide 4.596
IPC2_peptide 4.749
IPC2.peptide.svr19 4.739
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
116
116
116
116.0
13.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.724 ± 0.0
3.448 ± 0.0
6.897 ± 0.0
6.897 ± 0.0
6.897 ± 0.0
4.31 ± 0.0
1.724 ± 0.0
9.483 ± 0.0
4.31 ± 0.0
6.897 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.172 ± 0.0
6.897 ± 0.0
4.31 ± 0.0
2.586 ± 0.0
4.31 ± 0.0
2.586 ± 0.0
8.621 ± 0.0
6.897 ± 0.0
0.862 ± 0.0
5.172 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here