Klebsiella phage 1611E-K2-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jedunavirus; unclassified Jedunavirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S6PUF3|A0A3S6PUF3_9CAUD Phage_Mu_F domain-containing protein OS=Klebsiella phage 1611E-K2-1 OX=2047786 GN=1611EK21_7 PE=4 SV=1
MM1 pKa = 7.49TIIKK5 pKa = 10.09RR6 pKa = 11.84ADD8 pKa = 3.26LGRR11 pKa = 11.84PLTWDD16 pKa = 3.42EE17 pKa = 4.98LDD19 pKa = 5.79DD20 pKa = 5.05NFQQVDD26 pKa = 4.31DD27 pKa = 4.24LTAAASAAVLSASASATAAAGSATNSLNSANSAASSADD65 pKa = 3.35DD66 pKa = 3.63AAASATVAINALMNSTFEE84 pKa = 4.08PADD87 pKa = 3.51FDD89 pKa = 4.06FTSGGTLDD97 pKa = 3.38STDD100 pKa = 3.43RR101 pKa = 11.84NKK103 pKa = 10.89AVYY106 pKa = 10.32NPADD110 pKa = 3.86NNWYY114 pKa = 8.76SWSGILPKK122 pKa = 10.14IVTAATDD129 pKa = 3.6PTADD133 pKa = 4.05SNWKK137 pKa = 9.38PRR139 pKa = 11.84TDD141 pKa = 3.14QLLRR145 pKa = 11.84QNLASSVIPGTSLVTHH161 pKa = 6.92SDD163 pKa = 3.98GIHH166 pKa = 6.46LDD168 pKa = 3.6DD169 pKa = 5.37YY170 pKa = 11.7IEE172 pKa = 4.17IFNRR176 pKa = 11.84RR177 pKa = 11.84TKK179 pKa = 10.78FIMPEE184 pKa = 4.1DD185 pKa = 3.96FPGTDD190 pKa = 3.35TEE192 pKa = 4.38QLQSALSYY200 pKa = 10.84AKK202 pKa = 10.08SNRR205 pKa = 11.84VNVVLQAGKK214 pKa = 8.25TYY216 pKa = 10.81YY217 pKa = 9.2VTGSQGLEE225 pKa = 3.68VDD227 pKa = 3.66LGYY230 pKa = 11.4YY231 pKa = 10.34SFEE234 pKa = 4.27SPNGIAYY241 pKa = 9.24IDD243 pKa = 3.84FTGCTATYY251 pKa = 9.12CLWVHH256 pKa = 6.33SSRR259 pKa = 11.84PYY261 pKa = 10.05PDD263 pKa = 3.78GSEE266 pKa = 3.99NHH268 pKa = 5.81CTSMRR273 pKa = 11.84GIKK276 pKa = 9.86FKK278 pKa = 11.27SSVKK282 pKa = 10.76GIGQRR287 pKa = 11.84LLLTGNNNDD296 pKa = 4.15SSNGTYY302 pKa = 10.71NGDD305 pKa = 3.76CKK307 pKa = 10.58IEE309 pKa = 3.79NCMFSTADD317 pKa = 3.22IVLGASNSTWRR328 pKa = 11.84YY329 pKa = 9.87KK330 pKa = 10.6FINCGFMMEE339 pKa = 4.62STGGTYY345 pKa = 11.09AMHH348 pKa = 6.71FPAGISDD355 pKa = 3.7SGEE358 pKa = 3.71SVTFQNCKK366 pKa = 9.49IFDD369 pKa = 3.78MKK371 pKa = 10.86GCPILVEE378 pKa = 4.28CASFAIGMPGTSVLNTPIKK397 pKa = 9.55ITGSGAMVIMDD408 pKa = 4.09SAANIEE414 pKa = 4.36NPGASAWYY422 pKa = 9.56RR423 pKa = 11.84YY424 pKa = 10.67GEE426 pKa = 4.23VTGTGARR433 pKa = 11.84LILNGCTLVCNNPSLQTKK451 pKa = 8.88PLFYY455 pKa = 10.85VGANAFIDD463 pKa = 3.75VTLVKK468 pKa = 10.15TPGNDD473 pKa = 3.2YY474 pKa = 10.97LFQNGDD480 pKa = 2.96EE481 pKa = 4.31GLRR484 pKa = 11.84TFVEE488 pKa = 4.03GDD490 pKa = 3.68GYY492 pKa = 9.91VTASHH497 pKa = 7.33CIGDD501 pKa = 3.77ILSGVGNIPLHH512 pKa = 6.43KK513 pKa = 10.46SLNPTLNPGFEE524 pKa = 4.31TGDD527 pKa = 3.41LSSWSFNNQGSASQTCVVGTAYY549 pKa = 10.0KK550 pKa = 9.32KK551 pKa = 8.9TGTYY555 pKa = 8.69GARR558 pKa = 11.84MTSFGSLSCFLTQKK572 pKa = 10.58VKK574 pKa = 9.22VTQHH578 pKa = 5.65GYY580 pKa = 11.18YY581 pKa = 9.24STTCQINTITAGTGTTAGSLTITFYY606 pKa = 11.35NRR608 pKa = 11.84DD609 pKa = 3.51GNALQAGASSNFTNTPSGWQSVGRR633 pKa = 11.84FIQGRR638 pKa = 11.84VPQAAEE644 pKa = 3.78YY645 pKa = 10.57CEE647 pKa = 4.16VSFRR651 pKa = 11.84CRR653 pKa = 11.84EE654 pKa = 3.89GAVIDD659 pKa = 3.67VDD661 pKa = 3.64NFIINFTT668 pKa = 3.83

Molecular weight: 71.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S6PTV9|A0A3S6PTV9_9CAUD Putative DNA polymerase OS=Klebsiella phage 1611E-K2-1 OX=2047786 GN=1611EK21_13 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84AFRR5 pKa = 11.84FPRR8 pKa = 11.84SEE10 pKa = 3.74ARR12 pKa = 11.84HH13 pKa = 6.46DD14 pKa = 3.44VDD16 pKa = 4.37QPVGGVKK23 pKa = 10.06VCFSSLSHH31 pKa = 6.35VYY33 pKa = 10.08KK34 pKa = 10.63ISDD37 pKa = 3.36AVRR40 pKa = 11.84RR41 pKa = 11.84ASNPRR46 pKa = 11.84LLAMINEE53 pKa = 4.39SAMFGDD59 pKa = 3.8MMSRR63 pKa = 11.84FAKK66 pKa = 10.49SFLLSTLTLPLLSKK80 pKa = 10.83SRR82 pKa = 11.84IGVDD86 pKa = 3.22NSSMSLSSSFASISLDD102 pKa = 3.84KK103 pKa = 11.14LIIWSTGNSRR113 pKa = 11.84PFVTARR119 pKa = 11.84YY120 pKa = 8.8VLRR123 pKa = 11.84NRR125 pKa = 11.84SAVSHH130 pKa = 6.02HH131 pKa = 5.88VCAFRR136 pKa = 11.84LAKK139 pKa = 10.21KK140 pKa = 10.25SSLLMRR146 pKa = 11.84VSPYY150 pKa = 10.55FFFGSDD156 pKa = 3.52TLPPALNLKK165 pKa = 8.81CLCHH169 pKa = 6.3PAAFRR174 pKa = 11.84CEE176 pKa = 4.25PVPAPTPILSS186 pKa = 3.38

Molecular weight: 20.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

7239

186

785

425.8

47.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.551 ± 0.665

1.63 ± 0.246

6.382 ± 0.337

4.835 ± 0.399

3.896 ± 0.286

7.888 ± 0.62

2.017 ± 0.311

5.498 ± 0.21

5.318 ± 0.436

6.879 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.244

5.415 ± 0.493

4.006 ± 0.225

4.103 ± 0.428

5.484 ± 0.35

6.727 ± 0.522

6.824 ± 0.425

6.797 ± 0.329

1.533 ± 0.123

3.426 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski