Klebsiella phage KN1-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; Klebsiella virus KN1-1

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S5IBH4|A0A3S5IBH4_9CAUD Tail spike protein OS=Klebsiella phage KN1-1 OX=2282629 PE=4 SV=1
MM1 pKa = 7.38NMQDD5 pKa = 4.11AYY7 pKa = 10.62FGSAAEE13 pKa = 3.97LDD15 pKa = 4.2AVNEE19 pKa = 3.86MLAAIGEE26 pKa = 4.7SPVTTLDD33 pKa = 3.19EE34 pKa = 5.17DD35 pKa = 4.26GNADD39 pKa = 3.24VANARR44 pKa = 11.84RR45 pKa = 11.84ILNRR49 pKa = 11.84INRR52 pKa = 11.84QIQSKK57 pKa = 9.61GWAFNINEE65 pKa = 4.55SATLTPDD72 pKa = 2.99ASTGLIPFRR81 pKa = 11.84PAYY84 pKa = 10.44LSILGGQYY92 pKa = 10.17VNRR95 pKa = 11.84GGWVYY100 pKa = 11.2DD101 pKa = 3.54KK102 pKa = 11.07STGTDD107 pKa = 3.14TFSGPITVTLITLQDD122 pKa = 3.68YY123 pKa = 11.66DD124 pKa = 4.32EE125 pKa = 4.72MPEE128 pKa = 5.08CFRR131 pKa = 11.84QWIVTKK137 pKa = 10.66ASRR140 pKa = 11.84QFNSRR145 pKa = 11.84FFGAEE150 pKa = 3.73DD151 pKa = 3.76VEE153 pKa = 4.46NSLAQEE159 pKa = 3.88EE160 pKa = 4.75MEE162 pKa = 5.66ARR164 pKa = 11.84MACNEE169 pKa = 3.84YY170 pKa = 10.91EE171 pKa = 4.14MDD173 pKa = 3.84FGQYY177 pKa = 11.01NMLDD181 pKa = 3.06GDD183 pKa = 4.58AYY185 pKa = 10.18VQGLIGRR192 pKa = 4.48

Molecular weight: 21.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S5IBH1|A0A3S5IBH1_9CAUD Phage protein kinase OS=Klebsiella phage KN1-1 OX=2282629 PE=4 SV=1
MM1 pKa = 7.9PYY3 pKa = 10.3SSSNHH8 pKa = 5.51LSYY11 pKa = 11.19VPSSISFCLNSRR23 pKa = 11.84GCCLKK28 pKa = 10.6RR29 pKa = 11.84MPLAIPRR36 pKa = 11.84TSSMVSSTDD45 pKa = 2.99SSSIAMPTLSPCPLANPRR63 pKa = 11.84ACWRR67 pKa = 11.84TNVDD71 pKa = 4.43LPIPDD76 pKa = 4.8PEE78 pKa = 4.33VTMMTSPPRR87 pKa = 11.84APKK90 pKa = 10.02VRR92 pKa = 11.84SFSPGHH98 pKa = 5.51PEE100 pKa = 3.28KK101 pKa = 10.48RR102 pKa = 11.84YY103 pKa = 7.6PTLCSLVMASRR114 pKa = 11.84TRR116 pKa = 11.84SFMDD120 pKa = 3.77IAPMTPSGTHH130 pKa = 5.54GAAFHH135 pKa = 5.75IWSRR139 pKa = 11.84TSLPLPLSKK148 pKa = 10.63HH149 pKa = 5.34SLASFSVSTATWTLPGGRR167 pKa = 11.84TT168 pKa = 3.32

Molecular weight: 18.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

10158

67

1321

461.7

51.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.929 ± 0.598

0.935 ± 0.19

6.419 ± 0.212

6.94 ± 0.5

3.377 ± 0.173

8.043 ± 0.409

1.811 ± 0.274

4.883 ± 0.231

6.182 ± 0.425

8.259 ± 0.451

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.181

4.154 ± 0.233

3.997 ± 0.223

4.509 ± 0.343

5.562 ± 0.24

6.123 ± 0.402

5.572 ± 0.403

6.606 ± 0.41

1.437 ± 0.211

3.564 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski