Chlorobium sp.

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobium/Pelodictyon group; Chlorobium; unclassified Chlorobium

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2365 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A533TVJ6|A0A533TVJ6_CHLSQ RNA polymerase subunit sigma-24 OS=Chlorobium sp. OX=1095 GN=FDX30_06930 PE=4 SV=1
MM1 pKa = 7.04QKK3 pKa = 9.36WVCVPCGYY11 pKa = 10.9VYY13 pKa = 10.71DD14 pKa = 4.68PEE16 pKa = 5.62VGDD19 pKa = 4.42PDD21 pKa = 4.57SGIDD25 pKa = 3.37PGTPFEE31 pKa = 5.41DD32 pKa = 4.71LPEE35 pKa = 4.74DD36 pKa = 3.78WVCPVCGVDD45 pKa = 3.16KK46 pKa = 10.68TLFEE50 pKa = 5.37PYY52 pKa = 10.27DD53 pKa = 3.66EE54 pKa = 4.39NKK56 pKa = 10.11RR57 pKa = 11.84RR58 pKa = 3.56

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A533U647|A0A533U647_CHLSQ Sulfurtransferase complex subunit TusC OS=Chlorobium sp. OX=1095 GN=tusC PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 9.6RR3 pKa = 11.84TFQPRR8 pKa = 11.84NRR10 pKa = 11.84KK11 pKa = 8.8RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.88HH16 pKa = 4.5GFRR19 pKa = 11.84QRR21 pKa = 11.84MATKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 9.24VLSSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84HH40 pKa = 4.95SLSVSSQMGTTGRR53 pKa = 3.74

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2365

0

2365

745862

26

2967

315.4

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.05 ± 0.054

1.167 ± 0.02

5.271 ± 0.044

6.615 ± 0.059

4.566 ± 0.037

7.332 ± 0.048

2.125 ± 0.022

7.068 ± 0.047

5.706 ± 0.045

9.882 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.022

3.968 ± 0.036

4.226 ± 0.033

3.076 ± 0.024

5.499 ± 0.048

6.784 ± 0.042

5.09 ± 0.04

6.768 ± 0.044

1.051 ± 0.019

3.132 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski