Streptomyces phage Araceli

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TBA1|A0A5J6TBA1_9CAUD Uncharacterized protein OS=Streptomyces phage Araceli OX=2601641 GN=11 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 10.29GDD5 pKa = 3.66WEE7 pKa = 4.43VYY9 pKa = 9.65EE10 pKa = 5.23DD11 pKa = 4.05SDD13 pKa = 4.48RR14 pKa = 11.84EE15 pKa = 4.18PIEE18 pKa = 4.45TPVHH22 pKa = 5.17QQTACQLYY30 pKa = 7.78GHH32 pKa = 6.32TFEE35 pKa = 5.56TVTDD39 pKa = 4.06DD40 pKa = 4.8DD41 pKa = 4.82GNPLVFGTEE50 pKa = 3.82FMRR53 pKa = 11.84RR54 pKa = 11.84CNDD57 pKa = 3.21CGDD60 pKa = 3.84TYY62 pKa = 11.02TYY64 pKa = 11.2DD65 pKa = 3.74PDD67 pKa = 3.72EE68 pKa = 5.99DD69 pKa = 4.19EE70 pKa = 5.06EE71 pKa = 4.63STDD74 pKa = 3.39GG75 pKa = 4.56

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TAJ1|A0A5J6TAJ1_9CAUD Uncharacterized protein OS=Streptomyces phage Araceli OX=2601641 GN=56 PE=4 SV=1
MM1 pKa = 7.73TIPTGRR7 pKa = 11.84QRR9 pKa = 11.84RR10 pKa = 11.84LSPRR14 pKa = 11.84EE15 pKa = 3.55MRR17 pKa = 11.84GMPKK21 pKa = 10.01EE22 pKa = 3.64LRR24 pKa = 11.84EE25 pKa = 4.0IVKK28 pKa = 10.02IAQAAEE34 pKa = 3.49WDD36 pKa = 3.54VWKK39 pKa = 9.45TNRR42 pKa = 11.84SHH44 pKa = 7.34LRR46 pKa = 11.84LRR48 pKa = 11.84PPAGSTRR55 pKa = 11.84PRR57 pKa = 11.84CPEE60 pKa = 3.24GHH62 pKa = 6.6EE63 pKa = 4.07DD64 pKa = 3.34HH65 pKa = 7.15RR66 pKa = 11.84AGATTLTTGTTPSDD80 pKa = 3.31MRR82 pKa = 11.84GLDD85 pKa = 3.57NLRR88 pKa = 11.84ADD90 pKa = 3.41LRR92 pKa = 11.84GYY94 pKa = 10.33GLEE97 pKa = 4.36GVV99 pKa = 4.05

Molecular weight: 11.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

16942

40

1365

209.2

22.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.693 ± 0.582

1.068 ± 0.194

6.127 ± 0.286

6.304 ± 0.391

2.987 ± 0.178

9.19 ± 0.328

1.842 ± 0.173

4.079 ± 0.253

5.601 ± 0.612

7.284 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.13

2.993 ± 0.209

5.596 ± 0.278

3.465 ± 0.216

6.522 ± 0.371

4.846 ± 0.249

6.05 ± 0.259

6.918 ± 0.212

2.29 ± 0.222

2.756 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski