Staphylococcus virus 37
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZCA5|Q4ZCA5_9CAUD ORF092 OS=Staphylococcus virus 37 OX=320840 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.55 SFWLTYY8 pKa = 8.84 LFCFASTSVLTLITQDD24 pKa = 4.58 FIISAAWSLLLSLAVYY40 pKa = 10.17 LFFLVWYY47 pKa = 9.02 YY48 pKa = 11.02 EE49 pKa = 3.93 EE50 pKa = 6.14 DD51 pKa = 3.58 EE52 pKa = 4.44 TEE54 pKa = 4.0 EE55 pKa = 4.12 AVDD58 pKa = 5.01 DD59 pKa = 4.27 GEE61 pKa = 4.55 EE62 pKa = 4.37 YY63 pKa = 9.34 ITLFTIKK70 pKa = 10.45 YY71 pKa = 8.89
Molecular weight: 8.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.945
IPC2_protein 3.745
IPC_protein 3.554
Toseland 3.414
ProMoST 3.745
Dawson 3.554
Bjellqvist 3.732
Wikipedia 3.49
Rodwell 3.427
Grimsley 3.338
Solomon 3.49
Lehninger 3.452
Nozaki 3.706
DTASelect 3.795
Thurlkill 3.49
EMBOSS 3.503
Sillero 3.694
Patrickios 0.006
IPC_peptide 3.49
IPC2_peptide 3.656
IPC2.peptide.svr19 3.656
Protein with the highest isoelectric point:
>tr|Q4ZCC3|Q4ZCC3_9CAUD ORF006 OS=Staphylococcus virus 37 OX=320840 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.03 HH3 pKa = 5.45 YY4 pKa = 10.68 FCIITPATRR13 pKa = 11.84 LKK15 pKa = 10.17 VISMWATCEE24 pKa = 4.06 RR25 pKa = 11.84 TDD27 pKa = 3.51 SSYY30 pKa = 11.27 HH31 pKa = 5.3 NRR33 pKa = 11.84 LITVRR38 pKa = 11.84 KK39 pKa = 8.22 PRR41 pKa = 11.84 LFFHH45 pKa = 6.69 IVSTITIASPRR56 pKa = 11.84 SCDD59 pKa = 3.02 RR60 pKa = 11.84 FCRR63 pKa = 11.84 VAA65 pKa = 4.32
Molecular weight: 7.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.472
IPC_protein 10.043
Toseland 10.292
ProMoST 10.014
Dawson 10.452
Bjellqvist 10.218
Wikipedia 10.643
Rodwell 10.672
Grimsley 10.511
Solomon 10.54
Lehninger 10.511
Nozaki 10.409
DTASelect 10.175
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.409
Patrickios 10.496
IPC_peptide 10.54
IPC2_peptide 9.663
IPC2.peptide.svr19 8.342
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
14468
40
1001
187.9
21.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.543 ± 0.348
0.677 ± 0.114
6.082 ± 0.298
6.988 ± 0.414
4.451 ± 0.282
6.158 ± 0.386
1.88 ± 0.156
7.451 ± 0.294
8.343 ± 0.308
7.61 ± 0.312
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.447 ± 0.168
6.704 ± 0.384
2.979 ± 0.164
4.361 ± 0.227
4.154 ± 0.252
5.868 ± 0.278
6.124 ± 0.189
6.262 ± 0.215
1.237 ± 0.165
4.679 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here