Aeromonas virus phiO18P
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5X9G2|A5X9G2_9CAUD Uncharacterized protein OS=Aeromonas virus phiO18P OX=393598 GN=phiO18_11 PE=4 SV=1
MM1 pKa = 7.53 SIDD4 pKa = 4.63 AIHH7 pKa = 6.19 IAKK10 pKa = 9.93 RR11 pKa = 11.84 AEE13 pKa = 4.09 RR14 pKa = 11.84 AVLPLLTEE22 pKa = 4.28 LLATGEE28 pKa = 4.12 QEE30 pKa = 3.84 NRR32 pKa = 11.84 IALGEE37 pKa = 4.31 LYY39 pKa = 10.85 SGDD42 pKa = 3.75 EE43 pKa = 4.7 FIQVQLVVTSRR54 pKa = 11.84 PADD57 pKa = 3.88 LLDD60 pKa = 4.93 DD61 pKa = 4.67 DD62 pKa = 5.66 SVMGDD67 pKa = 3.43 EE68 pKa = 4.55 AA69 pKa = 5.68
Molecular weight: 7.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.384
IPC2_protein 4.126
IPC_protein 4.012
Toseland 3.821
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.897
Rodwell 3.846
Grimsley 3.745
Solomon 3.973
Lehninger 3.923
Nozaki 4.113
DTASelect 4.279
Thurlkill 3.872
EMBOSS 3.91
Sillero 4.126
Patrickios 3.897
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.053
Protein with the highest isoelectric point:
>tr|A5X9G6|A5X9G6_9CAUD Uncharacterized protein OS=Aeromonas virus phiO18P OX=393598 GN=phiO18_15 PE=4 SV=1
MM1 pKa = 7.54 SMSSKK6 pKa = 8.92 QTVSRR11 pKa = 11.84 EE12 pKa = 3.59 EE13 pKa = 3.72 YY14 pKa = 9.62 RR15 pKa = 11.84 RR16 pKa = 11.84 LDD18 pKa = 3.49 NRR20 pKa = 11.84 VTCILQQRR28 pKa = 11.84 WPANEE33 pKa = 3.37 ISQWVGVLKK42 pKa = 10.88 GKK44 pKa = 8.84 QQAVACAILRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 HH57 pKa = 5.7 PRR59 pKa = 11.84 PTSLALPTIVGDD71 pKa = 3.8 IPNPFQAKK79 pKa = 9.7 ANRR82 pKa = 11.84 PTVSVLTADD91 pKa = 3.43 GRR93 pKa = 11.84 SVGRR97 pKa = 11.84 RR98 pKa = 11.84 HH99 pKa = 6.4 IVDD102 pKa = 3.55 GLTPVAIDD110 pKa = 3.1 QSGTIRR116 pKa = 11.84 CAVTGRR122 pKa = 11.84 TLFIAPGSAIDD133 pKa = 3.79 RR134 pKa = 11.84 ANPGAAEE141 pKa = 4.01 QLNPTYY147 pKa = 10.36 RR148 pKa = 11.84 PALHH152 pKa = 6.01 QVVADD157 pKa = 3.93 HH158 pKa = 6.42 CQHH161 pKa = 6.15 VEE163 pKa = 3.68 AGEE166 pKa = 3.9
Molecular weight: 18.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.165
IPC_protein 9.575
Toseland 10.131
ProMoST 9.838
Dawson 10.292
Bjellqvist 10.043
Wikipedia 10.467
Rodwell 10.423
Grimsley 10.335
Solomon 10.379
Lehninger 10.35
Nozaki 10.248
DTASelect 9.999
Thurlkill 10.175
EMBOSS 10.54
Sillero 10.248
Patrickios 10.204
IPC_peptide 10.379
IPC2_peptide 9.399
IPC2.peptide.svr19 8.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
10386
47
776
230.8
25.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.986 ± 0.551
1.136 ± 0.15
5.661 ± 0.203
5.565 ± 0.364
3.1 ± 0.22
7.732 ± 0.318
2.446 ± 0.208
4.448 ± 0.215
4.554 ± 0.326
9.85 ± 0.457
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.59 ± 0.194
3.389 ± 0.312
4.92 ± 0.216
5.17 ± 0.257
6.644 ± 0.388
5.267 ± 0.3
5.96 ± 0.235
5.941 ± 0.228
2.08 ± 0.193
2.561 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here