Lactobacillus coleohominis 101-4-CHN
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1652 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7XWY4|C7XWY4_9LACO ABC 3 transport family protein OS=Lactobacillus coleohominis 101-4-CHN OX=575594 GN=HMPREF0501_01269 PE=3 SV=1
MM1 pKa = 7.71 GDD3 pKa = 3.42 YY4 pKa = 10.39 MSFTTVVINPRR15 pKa = 11.84 ITEE18 pKa = 4.01 WLTVDD23 pKa = 4.24 GSFSKK28 pKa = 11.06 AFLWEE33 pKa = 3.93 NGQIEE38 pKa = 4.52 ISTRR42 pKa = 11.84 QWSLHH47 pKa = 4.55 NSEE50 pKa = 4.28 YY51 pKa = 11.37 DD52 pKa = 3.69 FIIVIDD58 pKa = 3.79 YY59 pKa = 9.92 HH60 pKa = 6.52 VLIIVEE66 pKa = 4.35 DD67 pKa = 3.99 VVICGG72 pKa = 3.55
Molecular weight: 8.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.963
IPC2_protein 4.304
IPC_protein 4.139
Toseland 3.973
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.884
Solomon 4.101
Lehninger 4.05
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.266
Patrickios 1.964
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.141
Protein with the highest isoelectric point:
>tr|C7XXM4|C7XXM4_9LACO Uronate isomerase OS=Lactobacillus coleohominis 101-4-CHN OX=575594 GN=uxaC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.02 KK9 pKa = 7.4 RR10 pKa = 11.84 HH11 pKa = 5.36 RR12 pKa = 11.84 ARR14 pKa = 11.84 VHH16 pKa = 5.72 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1652
0
1652
497507
30
1834
301.2
33.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.764 ± 0.063
0.602 ± 0.017
5.939 ± 0.065
5.187 ± 0.057
4.065 ± 0.051
6.677 ± 0.056
2.577 ± 0.032
6.901 ± 0.064
6.21 ± 0.057
9.384 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.822 ± 0.033
5.102 ± 0.059
3.767 ± 0.033
5.176 ± 0.079
4.385 ± 0.055
5.563 ± 0.056
5.98 ± 0.061
7.165 ± 0.054
1.092 ± 0.023
3.642 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here