Prevotella sp. CAG:1092
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2513 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5P3B9|R5P3B9_9BACT Uncharacterized protein OS=Prevotella sp. CAG:1092 OX=1262919 GN=BN465_00373 PE=3 SV=1
MM1 pKa = 7.48 AKK3 pKa = 10.21 VIEE6 pKa = 4.11 GLYY9 pKa = 9.7 YY10 pKa = 10.21 SEE12 pKa = 3.62 SHH14 pKa = 6.61 EE15 pKa = 4.2 YY16 pKa = 10.52 VKK18 pKa = 11.19 VEE20 pKa = 3.78 GEE22 pKa = 3.93 YY23 pKa = 10.76 GYY25 pKa = 11.54 VGITDD30 pKa = 3.89 YY31 pKa = 11.33 AQHH34 pKa = 5.7 QLGNVVYY41 pKa = 10.93 VDD43 pKa = 3.87 MPEE46 pKa = 3.98 VDD48 pKa = 4.66 DD49 pKa = 5.73 DD50 pKa = 4.01 VTAGEE55 pKa = 4.08 EE56 pKa = 4.06 FGAVEE61 pKa = 4.31 SVKK64 pKa = 10.6 AASDD68 pKa = 4.57 LISPISGTVVEE79 pKa = 4.44 INEE82 pKa = 4.13 VLEE85 pKa = 5.07 DD86 pKa = 3.94 EE87 pKa = 4.93 PEE89 pKa = 4.25 QINKK93 pKa = 9.8 DD94 pKa = 3.42 AFANWIMKK102 pKa = 9.94 VKK104 pKa = 10.84 LSDD107 pKa = 3.26 TSEE110 pKa = 4.52 LEE112 pKa = 4.23 GLMSASQYY120 pKa = 11.17 EE121 pKa = 4.84 EE122 pKa = 3.55 ICKK125 pKa = 10.48
Molecular weight: 13.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.986
IPC_protein 3.91
Toseland 3.745
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.745
Rodwell 3.745
Grimsley 3.656
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.101
Thurlkill 3.77
EMBOSS 3.757
Sillero 4.024
Patrickios 0.998
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|R5P5M2|R5P5M2_9BACT Uncharacterized protein OS=Prevotella sp. CAG:1092 OX=1262919 GN=BN465_01122 PE=4 SV=1
MM1 pKa = 7.85 PNGKK5 pKa = 9.19 KK6 pKa = 10.25 KK7 pKa = 10.12 KK8 pKa = 7.0 GHH10 pKa = 6.14 KK11 pKa = 9.06 MATHH15 pKa = 6.13 KK16 pKa = 10.39 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.25 NRR25 pKa = 11.84 HH26 pKa = 4.69 KK27 pKa = 11.1 SKK29 pKa = 11.1
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2513
0
2513
840019
29
3119
334.3
37.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.328 ± 0.053
1.383 ± 0.018
6.032 ± 0.033
6.137 ± 0.043
4.288 ± 0.03
6.749 ± 0.052
1.941 ± 0.026
7.016 ± 0.04
7.112 ± 0.044
8.385 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.004 ± 0.022
5.629 ± 0.046
3.412 ± 0.024
3.357 ± 0.03
4.199 ± 0.034
6.083 ± 0.041
5.794 ± 0.042
6.467 ± 0.037
1.22 ± 0.016
4.455 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here