Soybean Putnam virus
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7HDV8|J7HDV8_9VIRU Aspartic protease OS=Soybean Putnam virus OX=1221449 PE=3 SV=1
MM1 pKa = 7.41 SLAEE5 pKa = 3.86 IQKK8 pKa = 10.89 AIDD11 pKa = 3.38 QVKK14 pKa = 10.11 RR15 pKa = 11.84 LAEE18 pKa = 4.05 QIEE21 pKa = 4.25 KK22 pKa = 10.65 LLEE25 pKa = 4.04 SSKK28 pKa = 10.11 TIVEE32 pKa = 3.99 ASSAKK37 pKa = 10.05 VIQDD41 pKa = 3.02 ITSKK45 pKa = 11.16 LEE47 pKa = 3.68 EE48 pKa = 4.16 CHH50 pKa = 6.52 CNKK53 pKa = 10.42 EE54 pKa = 3.91 ILDD57 pKa = 3.91 ALKK60 pKa = 10.95 SHH62 pKa = 7.21 DD63 pKa = 4.1 SKK65 pKa = 11.34 EE66 pKa = 4.21 KK67 pKa = 10.75 PEE69 pKa = 4.06 TQIVNSDD76 pKa = 3.53 KK77 pKa = 11.39 SPSTSSLQKK86 pKa = 9.92 YY87 pKa = 8.28 SFPNEE92 pKa = 3.87 YY93 pKa = 10.31 VGNAEE98 pKa = 5.0 LGSSGNPNAIVFPRR112 pKa = 11.84 RR113 pKa = 3.21
Molecular weight: 12.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.546
IPC2_protein 5.639
IPC_protein 5.55
Toseland 5.69
ProMoST 5.766
Dawson 5.639
Bjellqvist 5.703
Wikipedia 5.55
Rodwell 5.601
Grimsley 5.715
Solomon 5.639
Lehninger 5.614
Nozaki 5.817
DTASelect 5.957
Thurlkill 5.842
EMBOSS 5.779
Sillero 5.919
Patrickios 4.495
IPC_peptide 5.652
IPC2_peptide 5.893
IPC2.peptide.svr19 6.087
Protein with the highest isoelectric point:
>tr|J7H710|J7H710_9VIRU Coat protein OS=Soybean Putnam virus OX=1221449 PE=3 SV=1
MM1 pKa = 7.31 VPPNTNEE8 pKa = 3.76 ATQMPHH14 pKa = 6.4 IYY16 pKa = 10.16 KK17 pKa = 10.35 KK18 pKa = 10.98 GRR20 pKa = 11.84 ILKK23 pKa = 9.6 FKK25 pKa = 10.26 KK26 pKa = 10.24 LNEE29 pKa = 4.92 GIDD32 pKa = 3.49 RR33 pKa = 11.84 TNRR36 pKa = 11.84 KK37 pKa = 9.19 YY38 pKa = 10.98 LFSSHH43 pKa = 6.17 GSGIQTVVNHH53 pKa = 6.29 CNNLNQVVARR63 pKa = 11.84 NFLQLAKK70 pKa = 10.21 LLSYY74 pKa = 10.71 FGLSKK79 pKa = 10.91 DD80 pKa = 3.45 PSEE83 pKa = 5.75 DD84 pKa = 3.53 YY85 pKa = 11.37 SKK87 pKa = 11.25 DD88 pKa = 3.05 PSVFKK93 pKa = 10.83 RR94 pKa = 11.84 FFKK97 pKa = 10.65 DD98 pKa = 3.43 LPSTSFKK105 pKa = 10.94 KK106 pKa = 10.96 GGDD109 pKa = 3.29 KK110 pKa = 10.36 KK111 pKa = 10.98 DD112 pKa = 3.44 KK113 pKa = 11.5 NEE115 pKa = 3.93 ILEE118 pKa = 4.42 KK119 pKa = 9.91 LTSLIEE125 pKa = 4.05 KK126 pKa = 10.13 QGTKK130 pKa = 10.47 LRR132 pKa = 11.84 DD133 pKa = 3.49 LEE135 pKa = 4.47 SKK137 pKa = 10.22 IDD139 pKa = 3.71 NFVKK143 pKa = 10.71 NDD145 pKa = 3.23 ASKK148 pKa = 11.47 DD149 pKa = 3.82 DD150 pKa = 4.03 IKK152 pKa = 11.21 DD153 pKa = 3.21 LRR155 pKa = 11.84 IDD157 pKa = 3.07 VRR159 pKa = 11.84 RR160 pKa = 11.84 RR161 pKa = 11.84 LDD163 pKa = 4.26 SIDD166 pKa = 3.9 EE167 pKa = 4.16 SLKK170 pKa = 10.94 KK171 pKa = 10.56 IIGG174 pKa = 3.61
Molecular weight: 20.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.857
IPC2_protein 8.96
IPC_protein 8.843
Toseland 9.97
ProMoST 9.458
Dawson 10.087
Bjellqvist 9.663
Wikipedia 10.175
Rodwell 10.789
Grimsley 10.116
Solomon 10.101
Lehninger 10.087
Nozaki 9.94
DTASelect 9.663
Thurlkill 9.955
EMBOSS 10.335
Sillero 9.999
Patrickios 10.496
IPC_peptide 10.116
IPC2_peptide 8.009
IPC2.peptide.svr19 7.934
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2333
113
699
388.8
44.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.029 ± 0.474
1.586 ± 0.257
5.744 ± 0.404
7.287 ± 0.846
4.929 ± 0.271
4.158 ± 0.276
2.529 ± 0.537
7.072 ± 0.727
10.502 ± 0.405
8.401 ± 0.567
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.757 ± 0.237
5.872 ± 0.315
4.286 ± 0.37
4.715 ± 0.375
4.201 ± 0.256
8.616 ± 0.693
5.101 ± 0.271
5.272 ± 0.253
0.772 ± 0.194
3.172 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here