Glypta fumiferanae ichnovirus
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A2PZV3|A2PZV3_9VIRU GfV-B56-ORF1 OS=Glypta fumiferanae ichnovirus OX=419435 PE=4 SV=1
MM1 pKa = 7.91 EE2 pKa = 5.8 IFPMDD7 pKa = 3.9 GLFKK11 pKa = 10.88 KK12 pKa = 9.33 NTMGNNIFHH21 pKa = 7.42 EE22 pKa = 4.23 IAIEE26 pKa = 4.41 GSLLMLRR33 pKa = 11.84 RR34 pKa = 11.84 IRR36 pKa = 11.84 DD37 pKa = 3.68 NVNEE41 pKa = 3.94 QMDD44 pKa = 4.48 TYY46 pKa = 11.39 LSDD49 pKa = 3.59 TNDD52 pKa = 3.21 QGEE55 pKa = 4.45 TCIVIAADD63 pKa = 3.55 RR64 pKa = 11.84 HH65 pKa = 5.67 RR66 pKa = 11.84 GRR68 pKa = 11.84 LAIEE72 pKa = 4.68 LIEE75 pKa = 4.22 IFVGLGADD83 pKa = 3.79 INGTDD88 pKa = 3.79 NEE90 pKa = 4.81 GNTALHH96 pKa = 4.61 YY97 pKa = 9.52 TVFNEE102 pKa = 3.84 DD103 pKa = 3.09 HH104 pKa = 7.08 ALASWLYY111 pKa = 7.54 QQPGINLNAANHH123 pKa = 7.0 DD124 pKa = 3.95 EE125 pKa = 4.2 LTPLGLAIQLNIQGMKK141 pKa = 10.61 AFLDD145 pKa = 3.76 FLEE148 pKa = 4.39 AARR151 pKa = 11.84 AVLIEE156 pKa = 4.46 WNDD159 pKa = 3.45 SDD161 pKa = 6.55 DD162 pKa = 6.05 DD163 pKa = 6.41 DD164 pKa = 7.31 DD165 pKa = 7.37 DD166 pKa = 6.2 EE167 pKa = 8.11 DD168 pKa = 6.77 DD169 pKa = 6.01 DD170 pKa = 7.22 DD171 pKa = 7.34 DD172 pKa = 4.91 DD173 pKa = 4.86 VSTRR177 pKa = 11.84 RR178 pKa = 11.84 HH179 pKa = 4.64 GG180 pKa = 3.36
Molecular weight: 20.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.254
IPC2_protein 4.024
IPC_protein 4.024
Toseland 3.821
ProMoST 4.19
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.859
Grimsley 3.719
Solomon 4.012
Lehninger 3.973
Nozaki 4.126
DTASelect 4.38
Thurlkill 3.859
EMBOSS 3.961
Sillero 4.151
Patrickios 3.694
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.07
Protein with the highest isoelectric point:
>tr|A2PZR5|A2PZR5_9VIRU GfV-B16-ORF1 OS=Glypta fumiferanae ichnovirus OX=419435 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.14 LHH4 pKa = 6.06 VFIAINEE11 pKa = 4.22 LDD13 pKa = 3.64 VKK15 pKa = 10.23 FWIRR19 pKa = 11.84 NTPKK23 pKa = 10.66 LSLKK27 pKa = 9.58 CTEE30 pKa = 3.9 TRR32 pKa = 11.84 NWLWTNHH39 pKa = 5.34 KK40 pKa = 9.73 LANNRR45 pKa = 11.84 QEE47 pKa = 3.76 ITIPMSLRR55 pKa = 11.84 LLDD58 pKa = 3.56 KK59 pKa = 11.27 SRR61 pKa = 11.84 DD62 pKa = 3.61 QNVFLVLSPTIEE74 pKa = 4.11 SPRR77 pKa = 11.84 LGLSISLRR85 pKa = 11.84 LRR87 pKa = 11.84 LRR89 pKa = 11.84 LTLMVNSFPNQLVNFGQCVYY109 pKa = 11.22
Molecular weight: 12.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.293
IPC2_protein 9.458
IPC_protein 9.765
Toseland 10.657
ProMoST 10.233
Dawson 10.716
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 11.082
Grimsley 10.745
Solomon 10.818
Lehninger 10.804
Nozaki 10.643
DTASelect 10.35
Thurlkill 10.628
EMBOSS 11.038
Sillero 10.657
Patrickios 10.862
IPC_peptide 10.818
IPC2_peptide 9.297
IPC2.peptide.svr19 8.56
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
101
0
101
20567
77
972
203.6
23.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.439 ± 0.18
2.854 ± 0.286
5.271 ± 0.203
5.844 ± 0.174
4.906 ± 0.157
3.476 ± 0.142
2.694 ± 0.133
8.008 ± 0.179
7.133 ± 0.302
8.587 ± 0.237
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.049 ± 0.126
6.316 ± 0.168
3.423 ± 0.131
3.214 ± 0.148
5.771 ± 0.158
7.405 ± 0.246
5.543 ± 0.181
6.685 ± 0.302
1.332 ± 0.071
4.05 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here