Streptomyces phage ToastyFinz

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Raleighvirus; unclassified Raleighvirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0E628|A0A1V0E628_9CAUD Uncharacterized protein OS=Streptomyces phage ToastyFinz OX=1965452 GN=SEA_TOASTYFINZ_7 PE=4 SV=1
MM1 pKa = 7.72LPEE4 pKa = 5.33GIPTVRR10 pKa = 11.84VTGRR14 pKa = 11.84FLTPEE19 pKa = 4.61GKK21 pKa = 9.35PLAGQVIFRR30 pKa = 11.84APGMVTFGEE39 pKa = 4.16FDD41 pKa = 3.9VILGGPVAAPLDD53 pKa = 3.69STGAFEE59 pKa = 4.74VVLPATDD66 pKa = 3.86APGMIPTDD74 pKa = 2.84WSYY77 pKa = 12.04AVAEE81 pKa = 4.18QLAGVPMNRR90 pKa = 11.84TYY92 pKa = 10.81QVLLPAEE99 pKa = 4.44TPAVDD104 pKa = 4.44LADD107 pKa = 4.11IAPTDD112 pKa = 4.16PSTPTYY118 pKa = 9.92VAVRR122 pKa = 11.84GDD124 pKa = 3.27SAYY127 pKa = 10.19EE128 pKa = 3.88VAVEE132 pKa = 3.97AGFVGTVEE140 pKa = 3.69QWLASLIGPQGVKK153 pKa = 10.5GDD155 pKa = 3.83TGQTGAAGDD164 pKa = 3.9DD165 pKa = 3.75AYY167 pKa = 10.42EE168 pKa = 4.14VAVAAGFVGDD178 pKa = 3.97RR179 pKa = 11.84AAWLASLVGPRR190 pKa = 11.84GATGATGEE198 pKa = 4.21QGPPGTNGADD208 pKa = 3.86GADD211 pKa = 3.72GAPGVVQSVNGQSLAAVVLDD231 pKa = 4.06AADD234 pKa = 3.52VGAVPDD240 pKa = 4.27TAPGAPNGVAQLDD253 pKa = 3.96AAGKK257 pKa = 10.07VPAEE261 pKa = 4.02QLPTGTGGGAVDD273 pKa = 4.13SVNGEE278 pKa = 4.12TGVVVLDD285 pKa = 4.15AADD288 pKa = 3.76VGASPTGHH296 pKa = 6.04THH298 pKa = 5.15TAAAVGALATTARR311 pKa = 11.84GAANGVASLDD321 pKa = 3.24ASTRR325 pKa = 11.84VPIAQLPAAAGRR337 pKa = 11.84NMWTPQALGFAAWSCDD353 pKa = 3.33PYY355 pKa = 11.15TVANPVAKK363 pKa = 10.04YY364 pKa = 9.73LKK366 pKa = 8.5PQRR369 pKa = 11.84LFFVGFNITEE379 pKa = 4.23TTTVNRR385 pKa = 11.84LVMFARR391 pKa = 11.84GYY393 pKa = 11.17GGVSTNRR400 pKa = 11.84YY401 pKa = 8.59RR402 pKa = 11.84GAIYY406 pKa = 10.1RR407 pKa = 11.84DD408 pKa = 3.24TGAKK412 pKa = 9.18VVEE415 pKa = 4.48SAGVALTMAGQEE427 pKa = 4.21AGSMPAMVDD436 pKa = 3.1NHH438 pKa = 6.54IGAVPLTIASTSLAPGRR455 pKa = 11.84YY456 pKa = 5.14WAAWSLVTGGTADD469 pKa = 3.36FAFYY473 pKa = 10.21HH474 pKa = 5.52VQNEE478 pKa = 4.44APVATANFWMPGSPFARR495 pKa = 11.84AWYY498 pKa = 9.06TEE500 pKa = 3.75GQTNAALPATVSQTAAGALADD521 pKa = 3.77HH522 pKa = 7.89DD523 pKa = 4.32IPIMALANVV532 pKa = 3.79

Molecular weight: 53.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0E690|A0A1V0E690_9CAUD Uncharacterized protein OS=Streptomyces phage ToastyFinz OX=1965452 GN=SEA_TOASTYFINZ_51 PE=4 SV=1
MM1 pKa = 7.16ATEE4 pKa = 4.41KK5 pKa = 10.54RR6 pKa = 11.84NQKK9 pKa = 7.3TAPPKK14 pKa = 9.84PGTRR18 pKa = 11.84PSIRR22 pKa = 11.84VDD24 pKa = 3.39DD25 pKa = 4.07QLAADD30 pKa = 4.7LAVVMRR36 pKa = 11.84TDD38 pKa = 3.35VNLSDD43 pKa = 3.46AVRR46 pKa = 11.84RR47 pKa = 11.84AVRR50 pKa = 11.84QLADD54 pKa = 3.32MYY56 pKa = 9.47RR57 pKa = 11.84TAWAEE62 pKa = 3.77GVVAVGTAPTLLAYY76 pKa = 9.94QLQQDD81 pKa = 4.24PAMLPPRR88 pKa = 11.84PTPAPAPTSAYY99 pKa = 9.88DD100 pKa = 3.88ARR102 pKa = 11.84PQPSTPPVGHH112 pKa = 7.14PAAPVARR119 pKa = 11.84PWAPAPGRR127 pKa = 11.84GPQVTRR133 pKa = 11.84SGPFPGVPVRR143 pKa = 11.84RR144 pKa = 11.84PP145 pKa = 3.01

Molecular weight: 15.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12672

49

780

243.7

26.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.885 ± 0.655

0.679 ± 0.116

6.7 ± 0.312

5.997 ± 0.323

1.918 ± 0.148

8.546 ± 0.513

2.131 ± 0.178

3.054 ± 0.223

1.989 ± 0.24

8.365 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.705 ± 0.106

2.107 ± 0.176

6.763 ± 0.407

3.843 ± 0.184

8.909 ± 0.494

3.969 ± 0.199

6.282 ± 0.28

7.268 ± 0.325

1.87 ± 0.125

2.02 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski