Human feces smacovirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus humas3

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142J7I4|A0A142J7I4_9VIRU Replication associated protein OS=Human feces smacovirus 3 OX=1820159 PE=4 SV=1
MM1 pKa = 6.69ATNYY5 pKa = 10.66ASASYY10 pKa = 10.76QEE12 pKa = 4.29ILDD15 pKa = 3.88VNTVKK20 pKa = 11.05GNVTIIGIHH29 pKa = 5.68TPTGNTPVRR38 pKa = 11.84RR39 pKa = 11.84LSGFFSQFRR48 pKa = 11.84KK49 pKa = 9.6FRR51 pKa = 11.84YY52 pKa = 9.1KK53 pKa = 9.37GCSVQVVPAATLPADD68 pKa = 3.8PLQVGFEE75 pKa = 4.13AGEE78 pKa = 3.95NTIDD82 pKa = 3.9MRR84 pKa = 11.84DD85 pKa = 3.58MLNPILFHH93 pKa = 5.87GTHH96 pKa = 6.44GEE98 pKa = 4.08SLQIAWNNIFYY109 pKa = 10.93NHH111 pKa = 6.87DD112 pKa = 3.77RR113 pKa = 11.84FNSTDD118 pKa = 3.39TGSTGYY124 pKa = 10.12IEE126 pKa = 5.14GAGFDD131 pKa = 3.68ADD133 pKa = 4.39DD134 pKa = 3.81VNFANSTNSPVEE146 pKa = 3.83TQYY149 pKa = 11.47YY150 pKa = 8.64QAITDD155 pKa = 3.78RR156 pKa = 11.84TWRR159 pKa = 11.84KK160 pKa = 9.59FGIQSTFKK168 pKa = 10.76LPFMSPRR175 pKa = 11.84VWKK178 pKa = 10.49ASTVYY183 pKa = 10.42PILPNAYY190 pKa = 9.13QDD192 pKa = 3.57QGAMNTDD199 pKa = 3.06SFMGGEE205 pKa = 4.17SALPQGTFFEE215 pKa = 5.12PSLSRR220 pKa = 11.84RR221 pKa = 11.84VGAQPTEE228 pKa = 4.05ILSQHH233 pKa = 5.25WMSSGTTPLGWLPTVTMPVNSGSVYY258 pKa = 9.93PAPPTILPKK267 pKa = 10.13IFMGILVLPPSYY279 pKa = 10.47LQEE282 pKa = 3.97LYY284 pKa = 10.53FRR286 pKa = 11.84VIVTHH291 pKa = 5.85YY292 pKa = 10.86YY293 pKa = 9.28EE294 pKa = 4.74FKK296 pKa = 11.15GFTASLAPASQMPSNIGTYY315 pKa = 10.94DD316 pKa = 3.47NAVKK320 pKa = 8.78QTATQGAKK328 pKa = 9.63EE329 pKa = 4.03AMAIDD334 pKa = 4.19EE335 pKa = 5.22PEE337 pKa = 4.64DD338 pKa = 3.54STLEE342 pKa = 4.12SPTANILRR350 pKa = 11.84VSDD353 pKa = 4.04GVFF356 pKa = 2.89

Molecular weight: 39.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142J7I4|A0A142J7I4_9VIRU Replication associated protein OS=Human feces smacovirus 3 OX=1820159 PE=4 SV=1
MM1 pKa = 6.35VQAWMITAPRR11 pKa = 11.84THH13 pKa = 5.99VSKK16 pKa = 10.63RR17 pKa = 11.84AIRR20 pKa = 11.84IMIDD24 pKa = 3.07TNDD27 pKa = 3.48CKK29 pKa = 10.87KK30 pKa = 10.1WIVAKK35 pKa = 10.47EE36 pKa = 3.87RR37 pKa = 11.84GKK39 pKa = 10.41NGYY42 pKa = 7.35EE43 pKa = 3.32HH44 pKa = 5.57WQIRR48 pKa = 11.84IEE50 pKa = 4.12SSNTGFFEE58 pKa = 3.88WCKK61 pKa = 10.68LYY63 pKa = 10.51IPTAHH68 pKa = 6.08VEE70 pKa = 3.9KK71 pKa = 10.78AEE73 pKa = 4.15RR74 pKa = 11.84GVDD77 pKa = 3.37EE78 pKa = 4.63SRR80 pKa = 11.84YY81 pKa = 5.33EE82 pKa = 4.23TKK84 pKa = 10.2EE85 pKa = 3.83GQYY88 pKa = 11.09VLYY91 pKa = 9.11TDD93 pKa = 3.29RR94 pKa = 11.84VEE96 pKa = 4.03ILKK99 pKa = 9.93QRR101 pKa = 11.84FGKK104 pKa = 8.87MRR106 pKa = 11.84PNQIRR111 pKa = 11.84ALEE114 pKa = 4.21ALEE117 pKa = 4.15STNDD121 pKa = 3.4RR122 pKa = 11.84EE123 pKa = 4.26VLVWYY128 pKa = 10.07DD129 pKa = 3.09EE130 pKa = 4.29GGNVGKK136 pKa = 10.39SWLTGALWEE145 pKa = 4.84RR146 pKa = 11.84GLAYY150 pKa = 9.61YY151 pKa = 9.54VPPTVDD157 pKa = 2.8SVKK160 pKa = 11.22AMVQWVASCYY170 pKa = 9.73QSGGWRR176 pKa = 11.84PYY178 pKa = 10.81VIIDD182 pKa = 4.59IPRR185 pKa = 11.84SWKK188 pKa = 9.59WSEE191 pKa = 3.76QLYY194 pKa = 10.02VAIEE198 pKa = 4.26SIKK201 pKa = 11.03DD202 pKa = 3.15GLVYY206 pKa = 9.39DD207 pKa = 4.17TRR209 pKa = 11.84YY210 pKa = 9.55HH211 pKa = 5.6AQCINIRR218 pKa = 11.84GVKK221 pKa = 10.09VLVLTNTQPKK231 pKa = 9.97LDD233 pKa = 4.58KK234 pKa = 10.73LSQDD238 pKa = 2.67RR239 pKa = 11.84WRR241 pKa = 11.84ICVFRR246 pKa = 11.84GRR248 pKa = 11.84RR249 pKa = 11.84GSALIVTLWADD260 pKa = 4.28FYY262 pKa = 11.04PPLGGG267 pKa = 3.95

Molecular weight: 31.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

623

267

356

311.5

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.902 ± 0.538

0.963 ± 0.538

4.655 ± 0.127

5.136 ± 0.728

4.173 ± 1.36

7.384 ± 0.158

1.605 ± 0.063

6.26 ± 0.506

4.334 ± 1.202

6.421 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.411

4.334 ± 0.791

5.939 ± 1.297

4.494 ± 0.221

5.618 ± 1.55

6.902 ± 0.981

7.544 ± 1.36

7.223 ± 0.822

2.889 ± 1.17

4.655 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski