Agromyces sp. OV415

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces; unclassified Agromyces

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3639 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A495WSP6|A0A495WSP6_9MICO 4-hydroxybenzoate polyprenyltransferase OS=Agromyces sp. OV415 OX=2135463 GN=C7478_1627 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.96FLSTISVGALVASAGAVVLALSPVNPAAAADD33 pKa = 4.25LPNGIHH39 pKa = 6.34TNFEE43 pKa = 3.85IDD45 pKa = 3.3GNTAGVNDD53 pKa = 3.74WEE55 pKa = 4.75GVVGHH60 pKa = 6.75TPTAPYY66 pKa = 8.19GTSTGIIDD74 pKa = 3.88AQRR77 pKa = 11.84SSDD80 pKa = 3.26SGANYY85 pKa = 7.72TAHH88 pKa = 6.27TCGGTEE94 pKa = 4.04TSSVFPGGAKK104 pKa = 9.27IDD106 pKa = 4.39DD107 pKa = 4.4NPTVALINGTANAKK121 pKa = 10.1GDD123 pKa = 3.55ACTGGSAYY131 pKa = 10.06EE132 pKa = 4.1VVTVGGVPHH141 pKa = 7.15TIFYY145 pKa = 8.4TYY147 pKa = 7.58WTRR150 pKa = 11.84LSGNGDD156 pKa = 3.23MTSYY160 pKa = 10.98EE161 pKa = 4.06IFQGPEE167 pKa = 3.52PGRR170 pKa = 11.84GDD172 pKa = 3.73DD173 pKa = 4.29YY174 pKa = 11.45LIEE177 pKa = 4.35FNYY180 pKa = 10.68DD181 pKa = 3.22PAGTPTTDD189 pKa = 3.11VNVLKK194 pKa = 10.31WDD196 pKa = 3.47GSKK199 pKa = 9.78WVDD202 pKa = 2.99TGTTVYY208 pKa = 10.1FQAAVGANTDD218 pKa = 3.7TNASSGDD225 pKa = 3.29NDD227 pKa = 4.14TFGEE231 pKa = 4.18MAIDD235 pKa = 4.08LTASGLMPNDD245 pKa = 4.09GACRR249 pKa = 11.84TFLDD253 pKa = 3.61AGFITRR259 pKa = 11.84TGNKK263 pKa = 6.79QTATLQDD270 pKa = 3.69MLVASDD276 pKa = 4.63PLTITNCGGLVVKK289 pKa = 10.4KK290 pKa = 10.32ASNPAGQTAGGFSYY304 pKa = 11.15VVDD307 pKa = 3.7RR308 pKa = 11.84TGGGQVHH315 pKa = 7.65DD316 pKa = 4.31STLTGPTDD324 pKa = 3.72TVAGNAGLGATIAVGDD340 pKa = 3.63THH342 pKa = 6.14TWGNVFAGQDD352 pKa = 3.5YY353 pKa = 10.82RR354 pKa = 11.84LTEE357 pKa = 4.21TTPSVPWQLQSTVCTVTNPTSHH379 pKa = 6.08QVEE382 pKa = 5.16TYY384 pKa = 9.46TNQAFKK390 pKa = 10.66LYY392 pKa = 10.49VGATTTCVITNATSFVKK409 pKa = 9.2VTKK412 pKa = 10.26IAQGDD417 pKa = 4.1PTTFDD422 pKa = 3.44YY423 pKa = 10.61TVNGAPLQVAGGASSSLIAFTPGTPVTIDD452 pKa = 3.47EE453 pKa = 4.92LDD455 pKa = 3.88EE456 pKa = 4.89PGQPAWHH463 pKa = 6.69LMGIVCTGSSASVNLAAGTASFTTVAGQTIEE494 pKa = 3.99CTYY497 pKa = 10.78TNRR500 pKa = 11.84QDD502 pKa = 3.14GAIRR506 pKa = 11.84IVKK509 pKa = 9.54AVQGAGGDD517 pKa = 3.72FTFTRR522 pKa = 11.84NFGTNFTLSPGANGTAMTDD541 pKa = 4.1FASVTPGSYY550 pKa = 8.9TVTEE554 pKa = 4.35TTPAPAFDD562 pKa = 4.06LTGLVCTDD570 pKa = 3.91DD571 pKa = 3.92VAGGTASSTVVATGVSTINVDD592 pKa = 3.11PGEE595 pKa = 4.68LVTCTYY601 pKa = 11.15TNTQRR606 pKa = 11.84GTIIVDD612 pKa = 3.22KK613 pKa = 9.57VTNPVGSPTSFGFGLGGPDD632 pKa = 3.74TDD634 pKa = 4.18PAGNGAFSLTDD645 pKa = 3.26AAAPKK650 pKa = 9.91VFSYY654 pKa = 10.08QKK656 pKa = 9.77PGVYY660 pKa = 9.5TLTEE664 pKa = 4.26ADD666 pKa = 3.87LDD668 pKa = 4.04GWDD671 pKa = 4.18PTSMTCTGEE680 pKa = 4.09AASDD684 pKa = 3.56TDD686 pKa = 4.2LDD688 pKa = 4.09GVTTFTLQPGEE699 pKa = 4.37TVTCTVLNTATKK711 pKa = 10.7GKK713 pKa = 8.41VTVTKK718 pKa = 9.93SVPGMEE724 pKa = 3.98VWGPFAIEE732 pKa = 4.34LQDD735 pKa = 3.72APAGQVNPQNVSNVDD750 pKa = 4.07PVAEE754 pKa = 4.12WTDD757 pKa = 3.46LVVGQTYY764 pKa = 8.28TLVEE768 pKa = 4.11PTVAGWTTEE777 pKa = 3.97GFTCNVGTATATGWTFTVTPGLEE800 pKa = 4.25VSCTIVNVPVPGQVTVTKK818 pKa = 9.64TVAGVAAGTAWSFDD832 pKa = 3.56LTISPSAGVLPSATQEE848 pKa = 3.82VSGVGNTTDD857 pKa = 3.22SVTWTNLAVGQTYY870 pKa = 8.93TITEE874 pKa = 4.45DD875 pKa = 5.53LPDD878 pKa = 3.71GWTGGAVDD886 pKa = 4.79CAGPDD891 pKa = 3.76ADD893 pKa = 4.65PATPGDD899 pKa = 3.98QFTVTPGLVLSCTVRR914 pKa = 11.84NEE916 pKa = 3.93ASPASAKK923 pKa = 7.87VTKK926 pKa = 9.88TSVGAAGTFTFVLTPLNPPGAPIEE950 pKa = 4.26QTVTTPIGGGTGATTFAGLVPGTTYY975 pKa = 10.72SIDD978 pKa = 3.66EE979 pKa = 4.17QDD981 pKa = 4.26PGAGWVAGTLQCSVEE996 pKa = 4.16HH997 pKa = 7.06ADD999 pKa = 3.55GSGGSVTEE1007 pKa = 4.08SSFTVLAGDD1016 pKa = 4.97VFDD1019 pKa = 6.16CDD1021 pKa = 3.27ITNTANARR1029 pKa = 11.84IVIVKK1034 pKa = 9.95QVDD1037 pKa = 3.71GANMSFSFQGTWAGASLFQIPTVAGTGSKK1066 pKa = 8.89TFEE1069 pKa = 4.07NVTPGSYY1076 pKa = 8.96TVTEE1080 pKa = 4.39VLPSAYY1086 pKa = 9.41TGTLLSCVEE1095 pKa = 4.14EE1096 pKa = 4.22RR1097 pKa = 11.84AGGADD1102 pKa = 3.34GGSSAEE1108 pKa = 4.39DD1109 pKa = 3.21LVGTIDD1115 pKa = 4.73LDD1117 pKa = 3.83PGEE1120 pKa = 4.92TVTCTYY1126 pKa = 11.28SNTEE1130 pKa = 3.97LASITVDD1137 pKa = 3.12KK1138 pKa = 10.65VVPGAGSIPGGATTQFDD1155 pKa = 4.05FEE1157 pKa = 5.45LDD1159 pKa = 3.34GDD1161 pKa = 4.11ASDD1164 pKa = 4.29TFSLTDD1170 pKa = 3.18GATPYY1175 pKa = 9.74STGLVEE1181 pKa = 4.32PGEE1184 pKa = 4.2YY1185 pKa = 10.12SVTEE1189 pKa = 3.79LAEE1192 pKa = 4.62ANWSLADD1199 pKa = 4.0LQCVGTDD1206 pKa = 3.8GEE1208 pKa = 4.43NVEE1211 pKa = 4.96IDD1213 pKa = 3.96DD1214 pKa = 4.11ATATIQLGAGEE1225 pKa = 5.25HH1226 pKa = 4.7VTCTYY1231 pKa = 11.09TNVPDD1236 pKa = 3.78TGDD1239 pKa = 3.2VSVRR1243 pKa = 11.84KK1244 pKa = 9.59IVTDD1248 pKa = 3.61VPDD1251 pKa = 4.83GYY1253 pKa = 9.92PFEE1256 pKa = 4.29FTISITPTTGLEE1268 pKa = 3.7PDD1270 pKa = 3.49RR1271 pKa = 11.84AQEE1274 pKa = 4.08VNQDD1278 pKa = 3.56EE1279 pKa = 5.03TSVSWSGLQAGVQYY1293 pKa = 10.45TIAEE1297 pKa = 4.37DD1298 pKa = 4.92DD1299 pKa = 3.63ADD1301 pKa = 3.68GWTEE1305 pKa = 4.24GSVTCTGAGEE1315 pKa = 4.26DD1316 pKa = 4.36LADD1319 pKa = 4.17GPAFSFIATPGISVTCTVTNTPVRR1343 pKa = 11.84GSVQVTKK1350 pKa = 10.53SVQGLPEE1357 pKa = 4.22GQDD1360 pKa = 2.64WSFEE1364 pKa = 3.96IGIDD1368 pKa = 3.78PVPDD1372 pKa = 3.54YY1373 pKa = 10.1ATEE1376 pKa = 4.83PIGPQDD1382 pKa = 3.59SPQTVTDD1389 pKa = 4.36DD1390 pKa = 4.47EE1391 pKa = 4.43PVIWWDD1397 pKa = 3.26NLEE1400 pKa = 4.05IGEE1403 pKa = 4.71TYY1405 pKa = 9.93TLSEE1409 pKa = 4.39VEE1411 pKa = 4.68VPEE1414 pKa = 4.49GWTGGDD1420 pKa = 3.05ITCNVGEE1427 pKa = 4.39AGEE1430 pKa = 4.46GGTWSFTVTPGLEE1443 pKa = 4.52VICSLEE1449 pKa = 4.09NTVVAPVGEE1458 pKa = 4.09ILKK1461 pKa = 9.85EE1462 pKa = 3.89LLSAEE1467 pKa = 4.09QQADD1471 pKa = 3.95GTWFIDD1477 pKa = 3.48YY1478 pKa = 10.22RR1479 pKa = 11.84ITVTNQSPVVPLVYY1493 pKa = 10.66DD1494 pKa = 4.14LADD1497 pKa = 3.25QPFYY1501 pKa = 11.55GLGSVITASEE1511 pKa = 4.06AGGPDD1516 pKa = 3.75GPIASWDD1523 pKa = 3.48QVGPDD1528 pKa = 3.79VEE1530 pKa = 4.65SSLAEE1535 pKa = 4.4DD1536 pKa = 3.75ASVPAGEE1543 pKa = 4.17SDD1545 pKa = 4.03VYY1547 pKa = 10.21TLHH1550 pKa = 8.14IEE1552 pKa = 4.1ATVPAVVFQTGQDD1565 pKa = 3.23QCAEE1569 pKa = 4.09GSTTNGGFRR1578 pKa = 11.84NTAFLTWGEE1587 pKa = 4.56DD1588 pKa = 3.51GTDD1591 pKa = 3.33QSSDD1595 pKa = 3.35CDD1597 pKa = 3.82EE1598 pKa = 5.26PGMADD1603 pKa = 3.27MTKK1606 pKa = 9.25TLSDD1610 pKa = 3.86EE1611 pKa = 4.53PSLVTDD1617 pKa = 4.35PGDD1620 pKa = 3.64PNFGDD1625 pKa = 3.93WIVEE1629 pKa = 3.83YY1630 pKa = 10.84DD1631 pKa = 3.84VVVTNDD1637 pKa = 2.85SGKK1640 pKa = 10.52DD1641 pKa = 3.36LFYY1644 pKa = 11.17DD1645 pKa = 4.06LSDD1648 pKa = 3.5VPGFPDD1654 pKa = 3.73GVVITASATTDD1665 pKa = 3.12APGADD1670 pKa = 3.15VSGWNGDD1677 pKa = 3.7DD1678 pKa = 3.52VTVLVDD1684 pKa = 4.03DD1685 pKa = 4.44QPIAAAGEE1693 pKa = 4.37GGPTVHH1699 pKa = 6.73TFHH1702 pKa = 6.72ITVIADD1708 pKa = 3.37VVDD1711 pKa = 3.15ITEE1714 pKa = 4.37IDD1716 pKa = 3.82DD1717 pKa = 3.97VQCVAEE1723 pKa = 4.24TSGHH1727 pKa = 5.52GFFNGAEE1734 pKa = 4.25MVSGTIVDD1742 pKa = 4.21RR1743 pKa = 11.84DD1744 pKa = 4.23DD1745 pKa = 4.41DD1746 pKa = 4.31CVDD1749 pKa = 3.19IPIGRR1754 pKa = 11.84IQLLKK1759 pKa = 10.1TVDD1762 pKa = 3.17NSAFDD1767 pKa = 4.09GLDD1770 pKa = 3.3LEE1772 pKa = 4.98ALAEE1776 pKa = 4.15RR1777 pKa = 11.84FDD1779 pKa = 4.41VEE1781 pKa = 4.39TLTLLEE1787 pKa = 4.3SSDD1790 pKa = 3.54WNVSGSLFEE1799 pKa = 6.42GDD1801 pKa = 5.0DD1802 pKa = 3.55PANPLLRR1809 pKa = 11.84FIEE1812 pKa = 4.02EE1813 pKa = 4.43RR1814 pKa = 11.84GDD1816 pKa = 3.28QGIVFTVPLGNYY1828 pKa = 9.12RR1829 pKa = 11.84LAEE1832 pKa = 3.95QVVASQATHH1841 pKa = 7.05PLLSYY1846 pKa = 9.53YY1847 pKa = 9.66QASGWSCDD1855 pKa = 4.04DD1856 pKa = 4.95LDD1858 pKa = 4.23PADD1861 pKa = 5.04AIGTVVLGALTSCSLEE1877 pKa = 3.82NRR1879 pKa = 11.84AVLVDD1884 pKa = 3.25VGIEE1888 pKa = 3.77KK1889 pKa = 10.63SYY1891 pKa = 11.65DD1892 pKa = 3.31LGEE1895 pKa = 4.85GGDD1898 pKa = 4.11GAVEE1902 pKa = 4.31AGDD1905 pKa = 3.61SFDD1908 pKa = 4.02YY1909 pKa = 11.03VLEE1912 pKa = 4.17VTNNGTLDD1920 pKa = 3.58VGDD1923 pKa = 5.18LDD1925 pKa = 5.47VEE1927 pKa = 4.37DD1928 pKa = 5.55LLDD1931 pKa = 4.09EE1932 pKa = 4.47EE1933 pKa = 5.35LVPTGPAVFTDD1944 pKa = 3.68EE1945 pKa = 5.94LGDD1948 pKa = 4.33PDD1950 pKa = 5.47SDD1952 pKa = 3.27WTQTSDD1958 pKa = 2.99VTANAFTAHH1967 pKa = 5.97GVGPFAPGHH1976 pKa = 5.5VVTITIPVRR1985 pKa = 11.84MVEE1988 pKa = 4.41AGPTPTPDD1996 pKa = 3.0VVGPDD2001 pKa = 3.44DD2002 pKa = 4.24PAPVVEE2008 pKa = 5.55PVDD2011 pKa = 4.9LDD2013 pKa = 4.81DD2014 pKa = 5.55IPNEE2018 pKa = 3.76ACVAITAGEE2027 pKa = 4.17GALADD2032 pKa = 4.48LDD2034 pKa = 4.03PTNDD2038 pKa = 3.4CSEE2041 pKa = 4.45VEE2043 pKa = 4.4VPKK2046 pKa = 10.69QAIDD2050 pKa = 2.88SGAYY2054 pKa = 8.14VRR2056 pKa = 11.84CVNDD2060 pKa = 3.86VPWLYY2065 pKa = 11.51YY2066 pKa = 10.23DD2067 pKa = 3.5IQVSDD2072 pKa = 3.93SVEE2075 pKa = 4.1PGPITVTWTSADD2087 pKa = 3.21GTLTKK2092 pKa = 10.84EE2093 pKa = 3.64EE2094 pKa = 4.61TIPFDD2099 pKa = 3.77EE2100 pKa = 5.17LSGRR2104 pKa = 11.84LLWPGAEE2111 pKa = 3.9VDD2113 pKa = 3.71EE2114 pKa = 4.77NGIPFVFPGWRR2125 pKa = 11.84PITEE2129 pKa = 4.11EE2130 pKa = 4.58DD2131 pKa = 3.8LVNPPFPGDD2140 pKa = 3.35RR2141 pKa = 11.84FLDD2144 pKa = 4.71LILDD2148 pKa = 4.23EE2149 pKa = 5.46NVATYY2154 pKa = 8.44PWRR2157 pKa = 11.84GEE2159 pKa = 4.25VVTVNPDD2166 pKa = 2.92GTRR2169 pKa = 11.84TVEE2172 pKa = 4.15SFPATVTFSINPSQSVLAIYY2192 pKa = 8.64PQALPSCEE2200 pKa = 3.82IEE2202 pKa = 4.28RR2203 pKa = 11.84PPLVDD2208 pKa = 3.07IQKK2211 pKa = 8.15TASVSSAAPGSNFTYY2226 pKa = 8.89TLKK2229 pKa = 10.83VAATGTGAATPVEE2242 pKa = 4.43IFDD2245 pKa = 5.55EE2246 pKa = 4.61IPADD2250 pKa = 3.2IKK2252 pKa = 11.42VNSINTAGAPAFPRR2266 pKa = 11.84WEE2268 pKa = 4.52GCQVTGANASGYY2280 pKa = 10.71GGVLHH2285 pKa = 6.19CTLLGVLGPDD2295 pKa = 3.64YY2296 pKa = 8.86PTAPDD2301 pKa = 3.21VTLGVTVNPSTKK2313 pKa = 9.01VTSIANTGEE2322 pKa = 4.0VCYY2325 pKa = 10.73SDD2327 pKa = 5.42SEE2329 pKa = 4.43DD2330 pKa = 3.53TAVPPTTVCDD2340 pKa = 3.65EE2341 pKa = 4.41STVTVSVPQPLAATGFGGALYY2362 pKa = 10.33AWGAGALTLLGGLVVALTVLRR2383 pKa = 11.84RR2384 pKa = 11.84RR2385 pKa = 11.84RR2386 pKa = 11.84VMARR2390 pKa = 11.84SS2391 pKa = 3.24

Molecular weight:
247.36 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A495WJP3|A0A495WJP3_9MICO Regulatory LuxR family protein OS=Agromyces sp. OV415 OX=2135463 GN=C7478_3031 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight:
4.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3639

0

3639

1232372

29

3325

338.7

36.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.324 ± 0.059

0.454 ± 0.01

6.539 ± 0.042

5.613 ± 0.037

3.117 ± 0.024

9.354 ± 0.045

1.927 ± 0.019

4.194 ± 0.027

1.651 ± 0.024

9.942 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.529 ± 0.014

1.8 ± 0.02

5.701 ± 0.035

2.537 ± 0.022

7.474 ± 0.05

5.185 ± 0.029

5.972 ± 0.045

9.201 ± 0.037

1.56 ± 0.019

1.924 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski