Agromyces sp. OV415
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3639 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A495WSP6|A0A495WSP6_9MICO 4-hydroxybenzoate polyprenyltransferase OS=Agromyces sp. OV415 OX=2135463 GN=C7478_1627 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 9.96 FLSTISVGALVASAGAVVLALSPVNPAAAADD33 pKa = 4.25 LPNGIHH39 pKa = 6.34 TNFEE43 pKa = 3.85 IDD45 pKa = 3.3 GNTAGVNDD53 pKa = 3.74 WEE55 pKa = 4.75 GVVGHH60 pKa = 6.75 TPTAPYY66 pKa = 8.19 GTSTGIIDD74 pKa = 3.88 AQRR77 pKa = 11.84 SSDD80 pKa = 3.26 SGANYY85 pKa = 7.72 TAHH88 pKa = 6.27 TCGGTEE94 pKa = 4.04 TSSVFPGGAKK104 pKa = 9.27 IDD106 pKa = 4.39 DD107 pKa = 4.4 NPTVALINGTANAKK121 pKa = 10.1 GDD123 pKa = 3.55 ACTGGSAYY131 pKa = 10.06 EE132 pKa = 4.1 VVTVGGVPHH141 pKa = 7.15 TIFYY145 pKa = 8.4 TYY147 pKa = 7.58 WTRR150 pKa = 11.84 LSGNGDD156 pKa = 3.23 MTSYY160 pKa = 10.98 EE161 pKa = 4.06 IFQGPEE167 pKa = 3.52 PGRR170 pKa = 11.84 GDD172 pKa = 3.73 DD173 pKa = 4.29 YY174 pKa = 11.45 LIEE177 pKa = 4.35 FNYY180 pKa = 10.68 DD181 pKa = 3.22 PAGTPTTDD189 pKa = 3.11 VNVLKK194 pKa = 10.31 WDD196 pKa = 3.47 GSKK199 pKa = 9.78 WVDD202 pKa = 2.99 TGTTVYY208 pKa = 10.1 FQAAVGANTDD218 pKa = 3.7 TNASSGDD225 pKa = 3.29 NDD227 pKa = 4.14 TFGEE231 pKa = 4.18 MAIDD235 pKa = 4.08 LTASGLMPNDD245 pKa = 4.09 GACRR249 pKa = 11.84 TFLDD253 pKa = 3.61 AGFITRR259 pKa = 11.84 TGNKK263 pKa = 6.79 QTATLQDD270 pKa = 3.69 MLVASDD276 pKa = 4.63 PLTITNCGGLVVKK289 pKa = 10.4 KK290 pKa = 10.32 ASNPAGQTAGGFSYY304 pKa = 11.15 VVDD307 pKa = 3.7 RR308 pKa = 11.84 TGGGQVHH315 pKa = 7.65 DD316 pKa = 4.31 STLTGPTDD324 pKa = 3.72 TVAGNAGLGATIAVGDD340 pKa = 3.63 THH342 pKa = 6.14 TWGNVFAGQDD352 pKa = 3.5 YY353 pKa = 10.82 RR354 pKa = 11.84 LTEE357 pKa = 4.21 TTPSVPWQLQSTVCTVTNPTSHH379 pKa = 6.08 QVEE382 pKa = 5.16 TYY384 pKa = 9.46 TNQAFKK390 pKa = 10.66 LYY392 pKa = 10.49 VGATTTCVITNATSFVKK409 pKa = 9.2 VTKK412 pKa = 10.26 IAQGDD417 pKa = 4.1 PTTFDD422 pKa = 3.44 YY423 pKa = 10.61 TVNGAPLQVAGGASSSLIAFTPGTPVTIDD452 pKa = 3.47 EE453 pKa = 4.92 LDD455 pKa = 3.88 EE456 pKa = 4.89 PGQPAWHH463 pKa = 6.69 LMGIVCTGSSASVNLAAGTASFTTVAGQTIEE494 pKa = 3.99 CTYY497 pKa = 10.78 TNRR500 pKa = 11.84 QDD502 pKa = 3.14 GAIRR506 pKa = 11.84 IVKK509 pKa = 9.54 AVQGAGGDD517 pKa = 3.72 FTFTRR522 pKa = 11.84 NFGTNFTLSPGANGTAMTDD541 pKa = 4.1 FASVTPGSYY550 pKa = 8.9 TVTEE554 pKa = 4.35 TTPAPAFDD562 pKa = 4.06 LTGLVCTDD570 pKa = 3.91 DD571 pKa = 3.92 VAGGTASSTVVATGVSTINVDD592 pKa = 3.11 PGEE595 pKa = 4.68 LVTCTYY601 pKa = 11.15 TNTQRR606 pKa = 11.84 GTIIVDD612 pKa = 3.22 KK613 pKa = 9.57 VTNPVGSPTSFGFGLGGPDD632 pKa = 3.74 TDD634 pKa = 4.18 PAGNGAFSLTDD645 pKa = 3.26 AAAPKK650 pKa = 9.91 VFSYY654 pKa = 10.08 QKK656 pKa = 9.77 PGVYY660 pKa = 9.5 TLTEE664 pKa = 4.26 ADD666 pKa = 3.87 LDD668 pKa = 4.04 GWDD671 pKa = 4.18 PTSMTCTGEE680 pKa = 4.09 AASDD684 pKa = 3.56 TDD686 pKa = 4.2 LDD688 pKa = 4.09 GVTTFTLQPGEE699 pKa = 4.37 TVTCTVLNTATKK711 pKa = 10.7 GKK713 pKa = 8.41 VTVTKK718 pKa = 9.93 SVPGMEE724 pKa = 3.98 VWGPFAIEE732 pKa = 4.34 LQDD735 pKa = 3.72 APAGQVNPQNVSNVDD750 pKa = 4.07 PVAEE754 pKa = 4.12 WTDD757 pKa = 3.46 LVVGQTYY764 pKa = 8.28 TLVEE768 pKa = 4.11 PTVAGWTTEE777 pKa = 3.97 GFTCNVGTATATGWTFTVTPGLEE800 pKa = 4.25 VSCTIVNVPVPGQVTVTKK818 pKa = 9.64 TVAGVAAGTAWSFDD832 pKa = 3.56 LTISPSAGVLPSATQEE848 pKa = 3.82 VSGVGNTTDD857 pKa = 3.22 SVTWTNLAVGQTYY870 pKa = 8.93 TITEE874 pKa = 4.45 DD875 pKa = 5.53 LPDD878 pKa = 3.71 GWTGGAVDD886 pKa = 4.79 CAGPDD891 pKa = 3.76 ADD893 pKa = 4.65 PATPGDD899 pKa = 3.98 QFTVTPGLVLSCTVRR914 pKa = 11.84 NEE916 pKa = 3.93 ASPASAKK923 pKa = 7.87 VTKK926 pKa = 9.88 TSVGAAGTFTFVLTPLNPPGAPIEE950 pKa = 4.26 QTVTTPIGGGTGATTFAGLVPGTTYY975 pKa = 10.72 SIDD978 pKa = 3.66 EE979 pKa = 4.17 QDD981 pKa = 4.26 PGAGWVAGTLQCSVEE996 pKa = 4.16 HH997 pKa = 7.06 ADD999 pKa = 3.55 GSGGSVTEE1007 pKa = 4.08 SSFTVLAGDD1016 pKa = 4.97 VFDD1019 pKa = 6.16 CDD1021 pKa = 3.27 ITNTANARR1029 pKa = 11.84 IVIVKK1034 pKa = 9.95 QVDD1037 pKa = 3.71 GANMSFSFQGTWAGASLFQIPTVAGTGSKK1066 pKa = 8.89 TFEE1069 pKa = 4.07 NVTPGSYY1076 pKa = 8.96 TVTEE1080 pKa = 4.39 VLPSAYY1086 pKa = 9.41 TGTLLSCVEE1095 pKa = 4.14 EE1096 pKa = 4.22 RR1097 pKa = 11.84 AGGADD1102 pKa = 3.34 GGSSAEE1108 pKa = 4.39 DD1109 pKa = 3.21 LVGTIDD1115 pKa = 4.73 LDD1117 pKa = 3.83 PGEE1120 pKa = 4.92 TVTCTYY1126 pKa = 11.28 SNTEE1130 pKa = 3.97 LASITVDD1137 pKa = 3.12 KK1138 pKa = 10.65 VVPGAGSIPGGATTQFDD1155 pKa = 4.05 FEE1157 pKa = 5.45 LDD1159 pKa = 3.34 GDD1161 pKa = 4.11 ASDD1164 pKa = 4.29 TFSLTDD1170 pKa = 3.18 GATPYY1175 pKa = 9.74 STGLVEE1181 pKa = 4.32 PGEE1184 pKa = 4.2 YY1185 pKa = 10.12 SVTEE1189 pKa = 3.79 LAEE1192 pKa = 4.62 ANWSLADD1199 pKa = 4.0 LQCVGTDD1206 pKa = 3.8 GEE1208 pKa = 4.43 NVEE1211 pKa = 4.96 IDD1213 pKa = 3.96 DD1214 pKa = 4.11 ATATIQLGAGEE1225 pKa = 5.25 HH1226 pKa = 4.7 VTCTYY1231 pKa = 11.09 TNVPDD1236 pKa = 3.78 TGDD1239 pKa = 3.2 VSVRR1243 pKa = 11.84 KK1244 pKa = 9.59 IVTDD1248 pKa = 3.61 VPDD1251 pKa = 4.83 GYY1253 pKa = 9.92 PFEE1256 pKa = 4.29 FTISITPTTGLEE1268 pKa = 3.7 PDD1270 pKa = 3.49 RR1271 pKa = 11.84 AQEE1274 pKa = 4.08 VNQDD1278 pKa = 3.56 EE1279 pKa = 5.03 TSVSWSGLQAGVQYY1293 pKa = 10.45 TIAEE1297 pKa = 4.37 DD1298 pKa = 4.92 DD1299 pKa = 3.63 ADD1301 pKa = 3.68 GWTEE1305 pKa = 4.24 GSVTCTGAGEE1315 pKa = 4.26 DD1316 pKa = 4.36 LADD1319 pKa = 4.17 GPAFSFIATPGISVTCTVTNTPVRR1343 pKa = 11.84 GSVQVTKK1350 pKa = 10.53 SVQGLPEE1357 pKa = 4.22 GQDD1360 pKa = 2.64 WSFEE1364 pKa = 3.96 IGIDD1368 pKa = 3.78 PVPDD1372 pKa = 3.54 YY1373 pKa = 10.1 ATEE1376 pKa = 4.83 PIGPQDD1382 pKa = 3.59 SPQTVTDD1389 pKa = 4.36 DD1390 pKa = 4.47 EE1391 pKa = 4.43 PVIWWDD1397 pKa = 3.26 NLEE1400 pKa = 4.05 IGEE1403 pKa = 4.71 TYY1405 pKa = 9.93 TLSEE1409 pKa = 4.39 VEE1411 pKa = 4.68 VPEE1414 pKa = 4.49 GWTGGDD1420 pKa = 3.05 ITCNVGEE1427 pKa = 4.39 AGEE1430 pKa = 4.46 GGTWSFTVTPGLEE1443 pKa = 4.52 VICSLEE1449 pKa = 4.09 NTVVAPVGEE1458 pKa = 4.09 ILKK1461 pKa = 9.85 EE1462 pKa = 3.89 LLSAEE1467 pKa = 4.09 QQADD1471 pKa = 3.95 GTWFIDD1477 pKa = 3.48 YY1478 pKa = 10.22 RR1479 pKa = 11.84 ITVTNQSPVVPLVYY1493 pKa = 10.66 DD1494 pKa = 4.14 LADD1497 pKa = 3.25 QPFYY1501 pKa = 11.55 GLGSVITASEE1511 pKa = 4.06 AGGPDD1516 pKa = 3.75 GPIASWDD1523 pKa = 3.48 QVGPDD1528 pKa = 3.79 VEE1530 pKa = 4.65 SSLAEE1535 pKa = 4.4 DD1536 pKa = 3.75 ASVPAGEE1543 pKa = 4.17 SDD1545 pKa = 4.03 VYY1547 pKa = 10.21 TLHH1550 pKa = 8.14 IEE1552 pKa = 4.1 ATVPAVVFQTGQDD1565 pKa = 3.23 QCAEE1569 pKa = 4.09 GSTTNGGFRR1578 pKa = 11.84 NTAFLTWGEE1587 pKa = 4.56 DD1588 pKa = 3.51 GTDD1591 pKa = 3.33 QSSDD1595 pKa = 3.35 CDD1597 pKa = 3.82 EE1598 pKa = 5.26 PGMADD1603 pKa = 3.27 MTKK1606 pKa = 9.25 TLSDD1610 pKa = 3.86 EE1611 pKa = 4.53 PSLVTDD1617 pKa = 4.35 PGDD1620 pKa = 3.64 PNFGDD1625 pKa = 3.93 WIVEE1629 pKa = 3.83 YY1630 pKa = 10.84 DD1631 pKa = 3.84 VVVTNDD1637 pKa = 2.85 SGKK1640 pKa = 10.52 DD1641 pKa = 3.36 LFYY1644 pKa = 11.17 DD1645 pKa = 4.06 LSDD1648 pKa = 3.5 VPGFPDD1654 pKa = 3.73 GVVITASATTDD1665 pKa = 3.12 APGADD1670 pKa = 3.15 VSGWNGDD1677 pKa = 3.7 DD1678 pKa = 3.52 VTVLVDD1684 pKa = 4.03 DD1685 pKa = 4.44 QPIAAAGEE1693 pKa = 4.37 GGPTVHH1699 pKa = 6.73 TFHH1702 pKa = 6.72 ITVIADD1708 pKa = 3.37 VVDD1711 pKa = 3.15 ITEE1714 pKa = 4.37 IDD1716 pKa = 3.82 DD1717 pKa = 3.97 VQCVAEE1723 pKa = 4.24 TSGHH1727 pKa = 5.52 GFFNGAEE1734 pKa = 4.25 MVSGTIVDD1742 pKa = 4.21 RR1743 pKa = 11.84 DD1744 pKa = 4.23 DD1745 pKa = 4.41 DD1746 pKa = 4.31 CVDD1749 pKa = 3.19 IPIGRR1754 pKa = 11.84 IQLLKK1759 pKa = 10.1 TVDD1762 pKa = 3.17 NSAFDD1767 pKa = 4.09 GLDD1770 pKa = 3.3 LEE1772 pKa = 4.98 ALAEE1776 pKa = 4.15 RR1777 pKa = 11.84 FDD1779 pKa = 4.41 VEE1781 pKa = 4.39 TLTLLEE1787 pKa = 4.3 SSDD1790 pKa = 3.54 WNVSGSLFEE1799 pKa = 6.42 GDD1801 pKa = 5.0 DD1802 pKa = 3.55 PANPLLRR1809 pKa = 11.84 FIEE1812 pKa = 4.02 EE1813 pKa = 4.43 RR1814 pKa = 11.84 GDD1816 pKa = 3.28 QGIVFTVPLGNYY1828 pKa = 9.12 RR1829 pKa = 11.84 LAEE1832 pKa = 3.95 QVVASQATHH1841 pKa = 7.05 PLLSYY1846 pKa = 9.53 YY1847 pKa = 9.66 QASGWSCDD1855 pKa = 4.04 DD1856 pKa = 4.95 LDD1858 pKa = 4.23 PADD1861 pKa = 5.04 AIGTVVLGALTSCSLEE1877 pKa = 3.82 NRR1879 pKa = 11.84 AVLVDD1884 pKa = 3.25 VGIEE1888 pKa = 3.77 KK1889 pKa = 10.63 SYY1891 pKa = 11.65 DD1892 pKa = 3.31 LGEE1895 pKa = 4.85 GGDD1898 pKa = 4.11 GAVEE1902 pKa = 4.31 AGDD1905 pKa = 3.61 SFDD1908 pKa = 4.02 YY1909 pKa = 11.03 VLEE1912 pKa = 4.17 VTNNGTLDD1920 pKa = 3.58 VGDD1923 pKa = 5.18 LDD1925 pKa = 5.47 VEE1927 pKa = 4.37 DD1928 pKa = 5.55 LLDD1931 pKa = 4.09 EE1932 pKa = 4.47 EE1933 pKa = 5.35 LVPTGPAVFTDD1944 pKa = 3.68 EE1945 pKa = 5.94 LGDD1948 pKa = 4.33 PDD1950 pKa = 5.47 SDD1952 pKa = 3.27 WTQTSDD1958 pKa = 2.99 VTANAFTAHH1967 pKa = 5.97 GVGPFAPGHH1976 pKa = 5.5 VVTITIPVRR1985 pKa = 11.84 MVEE1988 pKa = 4.41 AGPTPTPDD1996 pKa = 3.0 VVGPDD2001 pKa = 3.44 DD2002 pKa = 4.24 PAPVVEE2008 pKa = 5.55 PVDD2011 pKa = 4.9 LDD2013 pKa = 4.81 DD2014 pKa = 5.55 IPNEE2018 pKa = 3.76 ACVAITAGEE2027 pKa = 4.17 GALADD2032 pKa = 4.48 LDD2034 pKa = 4.03 PTNDD2038 pKa = 3.4 CSEE2041 pKa = 4.45 VEE2043 pKa = 4.4 VPKK2046 pKa = 10.69 QAIDD2050 pKa = 2.88 SGAYY2054 pKa = 8.14 VRR2056 pKa = 11.84 CVNDD2060 pKa = 3.86 VPWLYY2065 pKa = 11.51 YY2066 pKa = 10.23 DD2067 pKa = 3.5 IQVSDD2072 pKa = 3.93 SVEE2075 pKa = 4.1 PGPITVTWTSADD2087 pKa = 3.21 GTLTKK2092 pKa = 10.84 EE2093 pKa = 3.64 EE2094 pKa = 4.61 TIPFDD2099 pKa = 3.77 EE2100 pKa = 5.17 LSGRR2104 pKa = 11.84 LLWPGAEE2111 pKa = 3.9 VDD2113 pKa = 3.71 EE2114 pKa = 4.77 NGIPFVFPGWRR2125 pKa = 11.84 PITEE2129 pKa = 4.11 EE2130 pKa = 4.58 DD2131 pKa = 3.8 LVNPPFPGDD2140 pKa = 3.35 RR2141 pKa = 11.84 FLDD2144 pKa = 4.71 LILDD2148 pKa = 4.23 EE2149 pKa = 5.46 NVATYY2154 pKa = 8.44 PWRR2157 pKa = 11.84 GEE2159 pKa = 4.25 VVTVNPDD2166 pKa = 2.92 GTRR2169 pKa = 11.84 TVEE2172 pKa = 4.15 SFPATVTFSINPSQSVLAIYY2192 pKa = 8.64 PQALPSCEE2200 pKa = 3.82 IEE2202 pKa = 4.28 RR2203 pKa = 11.84 PPLVDD2208 pKa = 3.07 IQKK2211 pKa = 8.15 TASVSSAAPGSNFTYY2226 pKa = 8.89 TLKK2229 pKa = 10.83 VAATGTGAATPVEE2242 pKa = 4.43 IFDD2245 pKa = 5.55 EE2246 pKa = 4.61 IPADD2250 pKa = 3.2 IKK2252 pKa = 11.42 VNSINTAGAPAFPRR2266 pKa = 11.84 WEE2268 pKa = 4.52 GCQVTGANASGYY2280 pKa = 10.71 GGVLHH2285 pKa = 6.19 CTLLGVLGPDD2295 pKa = 3.64 YY2296 pKa = 8.86 PTAPDD2301 pKa = 3.21 VTLGVTVNPSTKK2313 pKa = 9.01 VTSIANTGEE2322 pKa = 4.0 VCYY2325 pKa = 10.73 SDD2327 pKa = 5.42 SEE2329 pKa = 4.43 DD2330 pKa = 3.53 TAVPPTTVCDD2340 pKa = 3.65 EE2341 pKa = 4.41 STVTVSVPQPLAATGFGGALYY2362 pKa = 10.33 AWGAGALTLLGGLVVALTVLRR2383 pKa = 11.84 RR2384 pKa = 11.84 RR2385 pKa = 11.84 RR2386 pKa = 11.84 VMARR2390 pKa = 11.84 SS2391 pKa = 3.24
Molecular weight: 247.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.617
IPC_protein 3.668
Toseland 3.439
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.49
Grimsley 3.338
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.024
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.795
Patrickios 0.807
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A495WJP3|A0A495WJP3_9MICO Regulatory LuxR family protein OS=Agromyces sp. OV415 OX=2135463 GN=C7478_3031 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3639
0
3639
1232372
29
3325
338.7
36.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.324 ± 0.059
0.454 ± 0.01
6.539 ± 0.042
5.613 ± 0.037
3.117 ± 0.024
9.354 ± 0.045
1.927 ± 0.019
4.194 ± 0.027
1.651 ± 0.024
9.942 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.529 ± 0.014
1.8 ± 0.02
5.701 ± 0.035
2.537 ± 0.022
7.474 ± 0.05
5.185 ± 0.029
5.972 ± 0.045
9.201 ± 0.037
1.56 ± 0.019
1.924 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here