Euryarchaeota archaeon
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1149 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A482RAB5|A0A482RAB5_9EURY Uncharacterized protein OS=Euryarchaeota archaeon OX=2026739 GN=EOO41_01335 PE=4 SV=1
MM1 pKa = 7.32 VLLEE5 pKa = 4.42 VEE7 pKa = 4.1 WKK9 pKa = 9.97 VIYY12 pKa = 10.39 VGSSRR17 pKa = 11.84 DD18 pKa = 3.57 PIYY21 pKa = 11.08 DD22 pKa = 3.33 QVLDD26 pKa = 4.26 CFSMGG31 pKa = 3.39
Molecular weight: 3.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.879
IPC2_protein 4.24
IPC_protein 3.923
Toseland 3.757
ProMoST 4.139
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.91
Rodwell 3.783
Grimsley 3.694
Solomon 3.884
Lehninger 3.846
Nozaki 4.101
DTASelect 4.253
Thurlkill 3.859
EMBOSS 3.923
Sillero 4.05
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.957
Protein with the highest isoelectric point:
>tr|A0A482R8H9|A0A482R8H9_9EURY Uncharacterized protein (Fragment) OS=Euryarchaeota archaeon OX=2026739 GN=EOO41_01380 PE=4 SV=1
MM1 pKa = 7.35 HH2 pKa = 7.45 CLISVHH8 pKa = 6.64 SNTTRR13 pKa = 11.84 GVAAGLWPSRR23 pKa = 11.84 LRR25 pKa = 11.84 ASVRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 HH32 pKa = 4.92 TAATGVGSQNTSFFPPSPHH51 pKa = 6.52 NNLLHH56 pKa = 6.12 TPHH59 pKa = 6.98 TAGQAAQNAGSSSMRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 GARR79 pKa = 11.84 TFQGG83 pKa = 3.24
Molecular weight: 8.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.384
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.857
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1149
0
1149
416076
26
2087
362.1
37.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
17.699 ± 0.097
1.915 ± 0.044
4.423 ± 0.04
4.05 ± 0.06
2.094 ± 0.034
6.781 ± 0.077
3.034 ± 0.034
2.175 ± 0.028
1.624 ± 0.035
9.412 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.304 ± 0.027
1.726 ± 0.026
6.607 ± 0.073
3.583 ± 0.048
6.936 ± 0.055
9.462 ± 0.071
6.23 ± 0.047
7.418 ± 0.051
1.08 ± 0.023
1.445 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here