Staphylococcus phage 2638A
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZD31|Q4ZD31_9CAUD ORF040 OS=Staphylococcus phage 2638A OX=320836 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.61 YY3 pKa = 10.24 IITTAMILYY12 pKa = 9.66 IAYY15 pKa = 9.77 DD16 pKa = 3.4 YY17 pKa = 10.85 YY18 pKa = 10.68 IRR20 pKa = 11.84 LTANDD25 pKa = 5.29 DD26 pKa = 3.16 IDD28 pKa = 3.9 TFNLQDD34 pKa = 5.43 HH35 pKa = 7.16 IDD37 pKa = 3.84 LNNIRR42 pKa = 11.84 AEE44 pKa = 4.23 VTDD47 pKa = 3.7
Molecular weight: 5.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.833
IPC2_protein 4.062
IPC_protein 3.948
Toseland 3.732
ProMoST 4.024
Dawson 3.973
Bjellqvist 4.24
Wikipedia 3.986
Rodwell 3.783
Grimsley 3.643
Solomon 3.948
Lehninger 3.91
Nozaki 4.113
DTASelect 4.406
Thurlkill 3.834
EMBOSS 3.986
Sillero 4.088
Patrickios 0.693
IPC_peptide 3.948
IPC2_peptide 4.05
IPC2.peptide.svr19 4.029
Protein with the highest isoelectric point:
>tr|Q4ZD38|Q4ZD38_9CAUD ORF080 OS=Staphylococcus phage 2638A OX=320836 PE=4 SV=1
MM1 pKa = 7.42 GNEE4 pKa = 4.02 LNFLIEE10 pKa = 4.3 LNRR13 pKa = 11.84 EE14 pKa = 3.99 YY15 pKa = 11.18 LRR17 pKa = 11.84 LLILRR22 pKa = 11.84 KK23 pKa = 9.74 SRR25 pKa = 11.84 GSYY28 pKa = 10.26 GVTDD32 pKa = 3.87 AKK34 pKa = 10.52 LRR36 pKa = 11.84 RR37 pKa = 11.84 IGLVLRR43 pKa = 11.84 QSILEE48 pKa = 3.99 FEE50 pKa = 4.64 KK51 pKa = 11.04 GRR53 pKa = 11.84 MRR55 pKa = 11.84 NEE57 pKa = 3.57 NN58 pKa = 3.32
Molecular weight: 6.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.633
IPC_protein 10.555
Toseland 10.628
ProMoST 10.379
Dawson 10.745
Bjellqvist 10.467
Wikipedia 10.979
Rodwell 10.862
Grimsley 10.804
Solomon 10.877
Lehninger 10.847
Nozaki 10.599
DTASelect 10.467
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.672
IPC_peptide 10.891
IPC2_peptide 9.311
IPC2.peptide.svr19 8.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
12787
38
2008
224.3
25.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.452 ± 0.572
0.454 ± 0.116
6.35 ± 0.624
7.484 ± 0.627
4.121 ± 0.198
6.03 ± 0.498
1.791 ± 0.192
6.765 ± 0.242
9.189 ± 0.338
8.204 ± 0.324
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.667 ± 0.21
6.147 ± 0.326
2.44 ± 0.202
3.957 ± 0.226
4.575 ± 0.23
6.264 ± 0.404
5.553 ± 0.308
6.17 ± 0.241
1.275 ± 0.124
4.114 ± 0.364
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here