Coprobacillus sp. CAG:605
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1211 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5VMP0|R5VMP0_9FIRM Putative cytoplasmic protein OS=Coprobacillus sp. CAG:605 OX=1262855 GN=BN732_00448 PE=4 SV=1
MM1 pKa = 7.65 LMLACLSLGAYY12 pKa = 5.91 TTYY15 pKa = 11.65 LMVNDD20 pKa = 3.99 NAQKK24 pKa = 10.57 KK25 pKa = 7.45 AQKK28 pKa = 10.67 VIDD31 pKa = 4.44 DD32 pKa = 4.45 MLDD35 pKa = 3.25 EE36 pKa = 4.79 ATDD39 pKa = 3.97 FLNMDD44 pKa = 4.44 AYY46 pKa = 10.79
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.986
IPC_protein 3.834
Toseland 3.63
ProMoST 4.075
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.884
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.732
EMBOSS 3.884
Sillero 3.973
Patrickios 1.952
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|R5W1Y4|R5W1Y4_9FIRM Glyco_tran_28_C domain-containing protein OS=Coprobacillus sp. CAG:605 OX=1262855 GN=BN732_01202 PE=4 SV=1
MM1 pKa = 7.75 SNARR5 pKa = 11.84 NNARR9 pKa = 11.84 AKK11 pKa = 10.14 EE12 pKa = 3.94 NARR15 pKa = 11.84 NSAKK19 pKa = 10.77 NNARR23 pKa = 11.84 CNNSKK28 pKa = 10.18 CHH30 pKa = 5.87 ARR32 pKa = 11.84 NNARR36 pKa = 11.84 ANAKK40 pKa = 7.6 EE41 pKa = 3.96 TAEE44 pKa = 4.04
Molecular weight: 4.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.633
IPC_protein 10.409
Toseland 11.125
ProMoST 11.067
Dawson 11.155
Bjellqvist 10.921
Wikipedia 11.418
Rodwell 11.33
Grimsley 11.169
Solomon 11.418
Lehninger 11.359
Nozaki 11.111
DTASelect 10.906
Thurlkill 11.096
EMBOSS 11.55
Sillero 11.111
Patrickios 11.125
IPC_peptide 11.418
IPC2_peptide 10.248
IPC2.peptide.svr19 9.033
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1211
0
1211
355982
29
1693
294.0
33.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.712 ± 0.061
1.137 ± 0.026
6.067 ± 0.053
6.852 ± 0.067
4.029 ± 0.062
5.325 ± 0.087
1.369 ± 0.026
9.484 ± 0.077
9.503 ± 0.077
9.505 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.445 ± 0.034
7.539 ± 0.079
2.493 ± 0.038
2.266 ± 0.036
3.138 ± 0.048
6.384 ± 0.068
5.677 ± 0.075
6.056 ± 0.061
0.552 ± 0.018
5.468 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here