Croceibacterium mercuriale
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2758 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B2BXZ5|A0A0B2BXZ5_9SPHN K(+)-insensitive pyrophosphate-energized proton pump OS=Croceibacterium mercuriale OX=1572751 GN=hppA PE=3 SV=1
MM1 pKa = 7.53 SIYY4 pKa = 10.55 YY5 pKa = 10.01 VYY7 pKa = 9.94 ATNAGVQGWGKK18 pKa = 10.35 DD19 pKa = 3.23 WLGEE23 pKa = 3.95 DD24 pKa = 4.44 VIIEE28 pKa = 4.16 DD29 pKa = 3.26 EE30 pKa = 4.38 VMRR33 pKa = 11.84 FDD35 pKa = 5.25 FDD37 pKa = 4.82 DD38 pKa = 4.81 GSGACKK44 pKa = 9.98 FDD46 pKa = 4.13 IKK48 pKa = 10.95 VQYY51 pKa = 11.1 ADD53 pKa = 3.53 DD54 pKa = 4.89 AEE56 pKa = 5.78 AEE58 pKa = 4.28 LYY60 pKa = 10.22 EE61 pKa = 4.19 VDD63 pKa = 3.77 VCSVSHH69 pKa = 6.27 IDD71 pKa = 3.32 ARR73 pKa = 11.84 RR74 pKa = 11.84 GTMVVADD81 pKa = 4.36 DD82 pKa = 3.75
Molecular weight: 9.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.579
ProMoST 3.91
Dawson 3.795
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A0B2BXH7|A0A0B2BXH7_9SPHN Sodium:dicarboxylate symporter OS=Croceibacterium mercuriale OX=1572751 GN=PK98_09915 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.71 ILAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.5 VLCAA44 pKa = 3.88
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2758
0
2758
921998
29
2822
334.3
36.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.697 ± 0.072
0.747 ± 0.015
6.077 ± 0.029
5.297 ± 0.042
3.44 ± 0.026
9.232 ± 0.051
1.999 ± 0.03
4.507 ± 0.032
2.065 ± 0.036
10.285 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.323 ± 0.025
2.448 ± 0.037
5.509 ± 0.039
3.532 ± 0.027
7.599 ± 0.043
4.789 ± 0.037
5.522 ± 0.038
7.292 ± 0.032
1.515 ± 0.022
2.123 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here