Candidatus Magnetomorum sp. HK-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Candidatus Magnetomorum; unclassified Candidatus Magnetomorum

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1J1R8|A0A0N1J1R8_9DELT ABC transporter substrate-binding protein (Fragment) OS=Candidatus Magnetomorum sp. HK-1 OX=1509431 GN=MHK_000057 PE=4 SV=1
LL1 pKa = 7.48IIPVQVTDD9 pKa = 5.29HH10 pKa = 6.61EE11 pKa = 4.45ALSNRR16 pKa = 11.84FDD18 pKa = 3.1IHH20 pKa = 6.29ATLSPINDD28 pKa = 3.5QPSIIGQKK36 pKa = 9.33PLSGVEE42 pKa = 3.94DD43 pKa = 3.97TPFIIQLSDD52 pKa = 3.27ILVDD56 pKa = 4.87DD57 pKa = 4.73PDD59 pKa = 3.35NRR61 pKa = 11.84YY62 pKa = 9.14PSNFSLHH69 pKa = 6.48LLQGEE74 pKa = 4.47NYY76 pKa = 10.02SIANMTIIPSQNYY89 pKa = 8.42YY90 pKa = 10.76GSLVVPIQVNDD101 pKa = 3.88GEE103 pKa = 4.63LNSEE107 pKa = 4.24VFHH110 pKa = 7.23LSITMTPINDD120 pKa = 3.21IPVITGQKK128 pKa = 8.66QLVMDD133 pKa = 5.07EE134 pKa = 4.43DD135 pKa = 4.02THH137 pKa = 8.96LSILISDD144 pKa = 4.3IFIDD148 pKa = 6.0DD149 pKa = 4.49IDD151 pKa = 3.81NTDD154 pKa = 3.13IKK156 pKa = 11.09DD157 pKa = 3.62FSFMIKK163 pKa = 9.38TGLHH167 pKa = 5.57YY168 pKa = 10.63DD169 pKa = 3.62VNNQTVYY176 pKa = 10.24PQQDD180 pKa = 3.85FNGLLHH186 pKa = 6.61VPITVSDD193 pKa = 3.89KK194 pKa = 10.1TDD196 pKa = 2.95EE197 pKa = 4.3SAPYY201 pKa = 8.41TLQIMVQPMNDD212 pKa = 3.45PPVIIDD218 pKa = 3.54QYY220 pKa = 11.08PVITNEE226 pKa = 5.41DD227 pKa = 3.99MPCSLTLTDD236 pKa = 6.5LIIDD240 pKa = 4.72DD241 pKa = 5.02PDD243 pKa = 3.46EE244 pKa = 4.09QSFF247 pKa = 3.55

Molecular weight: 27.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0V1V4|A0A0N0V1V4_9DELT Uncharacterized protein (Fragment) OS=Candidatus Magnetomorum sp. HK-1 OX=1509431 GN=MHK_003131 PE=4 SV=1
SS1 pKa = 6.98CRR3 pKa = 11.84GEE5 pKa = 3.84PCVRR9 pKa = 11.84PRR11 pKa = 11.84TKK13 pKa = 10.35NVCEE17 pKa = 3.65QSFEE21 pKa = 4.38GEE23 pKa = 4.11HH24 pKa = 6.18KK25 pKa = 10.66VRR27 pKa = 11.84PLHH30 pKa = 7.1SEE32 pKa = 4.11GEE34 pKa = 4.49HH35 pKa = 6.19KK36 pKa = 10.49VRR38 pKa = 11.84PYY40 pKa = 9.94IWRR43 pKa = 11.84ANTRR47 pKa = 11.84FAPTFGGRR55 pKa = 11.84TQGSPLHH62 pKa = 6.77LEE64 pKa = 4.57GEE66 pKa = 4.65HH67 pKa = 6.52KK68 pKa = 10.17VRR70 pKa = 11.84PYY72 pKa = 10.26IRR74 pKa = 11.84RR75 pKa = 11.84ANIRR79 pKa = 11.84FAPTFGGRR87 pKa = 11.84TQGSAPTFGGRR98 pKa = 11.84TQGSPLHH105 pKa = 6.72SEE107 pKa = 4.55GEE109 pKa = 4.45HH110 pKa = 6.08KK111 pKa = 10.51VRR113 pKa = 11.84PYY115 pKa = 10.58IGFLTLII122 pKa = 4.39

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10924

0

10924

4167494

24

26480

381.5

43.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.586 ± 0.031

1.115 ± 0.014

6.233 ± 0.038

5.956 ± 0.028

4.707 ± 0.024

5.837 ± 0.045

2.005 ± 0.015

9.142 ± 0.029

7.151 ± 0.056

8.778 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.015

6.341 ± 0.028

3.619 ± 0.029

3.948 ± 0.019

3.487 ± 0.028

7.619 ± 0.041

5.882 ± 0.05

5.394 ± 0.02

1.114 ± 0.011

3.892 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski