Rhinolophus associated gemykibivirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus rhina1

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3MCK4|A0A0D3MCK4_9VIRU Rep2 OS=Rhinolophus associated gemykibivirus 1 OX=2004965 PE=4 SV=1
MM1 pKa = 7.7GKK3 pKa = 7.45TLWARR8 pKa = 11.84SHH10 pKa = 6.62GSHH13 pKa = 7.02AYY15 pKa = 10.06FGGLFSLDD23 pKa = 3.79EE24 pKa = 4.41PLQGANYY31 pKa = 10.76AVFDD35 pKa = 5.42DD36 pKa = 4.4INGGIQFFPQYY47 pKa = 10.22KK48 pKa = 8.34WWLGHH53 pKa = 5.19QAQFYY58 pKa = 9.15ATDD61 pKa = 3.41KK62 pKa = 10.98YY63 pKa = 10.53KK64 pKa = 10.88GKK66 pKa = 10.54RR67 pKa = 11.84LIHH70 pKa = 6.07WGKK73 pKa = 9.44PSIWVANDD81 pKa = 3.34DD82 pKa = 3.62PRR84 pKa = 11.84EE85 pKa = 3.83QHH87 pKa = 5.94GADD90 pKa = 4.42RR91 pKa = 11.84EE92 pKa = 4.27WLEE95 pKa = 4.12ANCLFVYY102 pKa = 9.25VHH104 pKa = 6.6SPLYY108 pKa = 10.85SNLVV112 pKa = 3.3

Molecular weight: 12.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3MCK4|A0A0D3MCK4_9VIRU Rep2 OS=Rhinolophus associated gemykibivirus 1 OX=2004965 PE=4 SV=1
MM1 pKa = 7.32TFSFNARR8 pKa = 11.84YY9 pKa = 9.72ALLTYY14 pKa = 8.83AQCGTLDD21 pKa = 3.4PWSVVHH27 pKa = 7.01HH28 pKa = 5.91IAEE31 pKa = 4.2LRR33 pKa = 11.84GEE35 pKa = 4.41CIVARR40 pKa = 11.84EE41 pKa = 4.0AHH43 pKa = 6.56ADD45 pKa = 3.58GGTHH49 pKa = 6.05LHH51 pKa = 6.21SFVDD55 pKa = 4.35FGRR58 pKa = 11.84KK59 pKa = 8.02FRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84TSIFDD69 pKa = 3.44VDD71 pKa = 3.42NHH73 pKa = 6.44HH74 pKa = 7.22PNVSATHH81 pKa = 4.54STPRR85 pKa = 11.84EE86 pKa = 4.05GFDD89 pKa = 3.51YY90 pKa = 10.91ACKK93 pKa = 10.75DD94 pKa = 3.18GDD96 pKa = 3.8IVAGGLSRR104 pKa = 11.84PDD106 pKa = 3.47AGAVPAKK113 pKa = 9.78HH114 pKa = 6.72DD115 pKa = 3.41RR116 pKa = 11.84WHH118 pKa = 7.68DD119 pKa = 3.08ITAAKK124 pKa = 10.16SRR126 pKa = 11.84EE127 pKa = 4.08EE128 pKa = 3.95FFQLLLEE135 pKa = 4.32HH136 pKa = 7.27APRR139 pKa = 11.84DD140 pKa = 3.5LCTSFTSLSKK150 pKa = 10.8YY151 pKa = 9.82ADD153 pKa = 2.63WMYY156 pKa = 11.4RR157 pKa = 11.84DD158 pKa = 4.69DD159 pKa = 4.36PAPYY163 pKa = 10.12KK164 pKa = 10.38HH165 pKa = 7.09DD166 pKa = 3.77NQLEE170 pKa = 4.27SSLGPFPEE178 pKa = 4.79LGSWVQGSINEE189 pKa = 4.02LSVGKK194 pKa = 10.01SCPSQPAPAPHH205 pKa = 6.87KK206 pKa = 9.51GAPGGPSQGAPPPWGRR222 pKa = 11.84VARR225 pKa = 11.84GRR227 pKa = 11.84WSISGG232 pKa = 3.46

Molecular weight: 25.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

344

112

232

172.0

19.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.302 ± 0.672

1.744 ± 0.451

6.686 ± 0.231

4.07 ± 0.264

5.233 ± 0.54

9.302 ± 0.275

5.523 ± 0.088

3.198 ± 0.198

4.07 ± 0.683

7.267 ± 0.881

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.872 ± 0.011

2.907 ± 0.826

6.977 ± 1.332

3.488 ± 0.991

5.814 ± 1.189

8.14 ± 1.475

3.488 ± 0.903

4.651 ± 0.099

3.488 ± 0.991

3.779 ± 1.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski