Chelonia mydas papillomavirus 1
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6RUP3|B6RUP3_9PAPI Replication protein OS=Chelonia mydas papillomavirus 1 OX=485242 GN=E1 PE=4 SV=1
MM1 pKa = 7.71 HH2 pKa = 8.17 DD3 pKa = 3.85 KK4 pKa = 10.76 LQPQKK9 pKa = 11.01 GCLSRR14 pKa = 11.84 YY15 pKa = 9.07 ICACCRR21 pKa = 11.84 QEE23 pKa = 4.6 VDD25 pKa = 3.97 FAQQEE30 pKa = 4.63 VCVMLSDD37 pKa = 3.68 QLGMLVCRR45 pKa = 11.84 SCEE48 pKa = 3.93 QSLLPHH54 pKa = 6.6 EE55 pKa = 4.88 LSDD58 pKa = 5.05 AISHH62 pKa = 6.49 ACMTEE67 pKa = 3.9 VSVSAGFDD75 pKa = 3.37 LHH77 pKa = 8.86 SDD79 pKa = 3.76 DD80 pKa = 5.95 SDD82 pKa = 3.65 LASYY86 pKa = 11.33 SDD88 pKa = 3.23 ISDD91 pKa = 3.36 WDD93 pKa = 3.92 SEE95 pKa = 4.33 EE96 pKa = 5.67 SPDD99 pKa = 4.08 TDD101 pKa = 3.2 TDD103 pKa = 3.97 FSGSHH108 pKa = 5.86
Molecular weight: 11.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.896
IPC2_protein 4.024
IPC_protein 3.999
Toseland 3.795
ProMoST 4.151
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.935
Rodwell 3.834
Grimsley 3.706
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.355
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.126
Patrickios 0.439
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>tr|B6RUP5|B6RUP5_9PAPI Putative E4 protein OS=Chelonia mydas papillomavirus 1 OX=485242 GN=E4 PE=4 SV=1
MM1 pKa = 7.42 MDD3 pKa = 3.4 ANQRR7 pKa = 11.84 LMEE10 pKa = 4.12 VQNIQLSIIEE20 pKa = 4.32 QDD22 pKa = 3.19 SHH24 pKa = 5.6 TLGSILEE31 pKa = 4.58 FYY33 pKa = 10.93 KK34 pKa = 10.93 AMKK37 pKa = 10.32 VEE39 pKa = 3.98 YY40 pKa = 9.96 LLLAAARR47 pKa = 11.84 KK48 pKa = 9.53 KK49 pKa = 10.43 GRR51 pKa = 11.84 LHH53 pKa = 7.37 IGVQRR58 pKa = 11.84 VPPMQVSEE66 pKa = 4.3 TKK68 pKa = 10.33 YY69 pKa = 10.38 RR70 pKa = 11.84 EE71 pKa = 3.92 ASTMIVLIEE80 pKa = 4.59 SLMQSQFRR88 pKa = 11.84 DD89 pKa = 3.27 RR90 pKa = 11.84 KK91 pKa = 9.66 FSLHH95 pKa = 5.72 EE96 pKa = 3.95 LQYY99 pKa = 10.61 SLVMTPPEE107 pKa = 4.1 YY108 pKa = 9.85 TVKK111 pKa = 10.48 QGPKK115 pKa = 9.23 QVYY118 pKa = 7.66 ITYY121 pKa = 9.99 SDD123 pKa = 3.95 PSQTTEE129 pKa = 5.15 QFTKK133 pKa = 9.77 WKK135 pKa = 8.55 TILYY139 pKa = 7.98 QTDD142 pKa = 3.38 DD143 pKa = 3.17 WSEE146 pKa = 4.12 RR147 pKa = 11.84 PSLPGTHH154 pKa = 6.79 DD155 pKa = 3.8 PKK157 pKa = 10.67 WFLAHH162 pKa = 6.76 TLTDD166 pKa = 3.51 KK167 pKa = 11.42 NGLFIVDD174 pKa = 3.51 KK175 pKa = 11.34 SGDD178 pKa = 3.19 KK179 pKa = 10.46 DD180 pKa = 3.7 YY181 pKa = 11.81 YY182 pKa = 11.5 AFFSTKK188 pKa = 9.65 EE189 pKa = 3.85 PSSAVARR196 pKa = 11.84 GQWTISSTVPAAAALSRR213 pKa = 11.84 QPSPRR218 pKa = 11.84 RR219 pKa = 11.84 SNSPNLDD226 pKa = 3.13 PCTRR230 pKa = 11.84 SRR232 pKa = 11.84 GSHH235 pKa = 6.37 CGGVSPSNPDD245 pKa = 3.35 TPDD248 pKa = 3.19 TAGVRR253 pKa = 11.84 PRR255 pKa = 11.84 DD256 pKa = 3.57 RR257 pKa = 11.84 TGGLGTPPKK266 pKa = 9.77 SARR269 pKa = 11.84 GGGGRR274 pKa = 11.84 GQSTGGSKK282 pKa = 10.19 LQRR285 pKa = 11.84 GPRR288 pKa = 11.84 VTPRR292 pKa = 11.84 SSRR295 pKa = 11.84 QSKK298 pKa = 9.2 EE299 pKa = 3.86 KK300 pKa = 11.06 NPVSPAEE307 pKa = 3.88 VGQNRR312 pKa = 11.84 KK313 pKa = 6.56 TVSGPGTRR321 pKa = 11.84 LDD323 pKa = 3.51 RR324 pKa = 11.84 LIRR327 pKa = 11.84 EE328 pKa = 4.32 ARR330 pKa = 11.84 DD331 pKa = 3.33 PPGVIIEE338 pKa = 4.75 GSTSQIKK345 pKa = 9.77 HH346 pKa = 4.82 LRR348 pKa = 11.84 RR349 pKa = 11.84 RR350 pKa = 11.84 IQLGPLKK357 pKa = 9.73 YY358 pKa = 10.59 LRR360 pKa = 11.84 VTSTWHH366 pKa = 5.73 WIVNKK371 pKa = 10.07 KK372 pKa = 7.2 VQKK375 pKa = 7.36 PCKK378 pKa = 8.9 MIVVFSNNAEE388 pKa = 3.98 RR389 pKa = 11.84 QTFLHH394 pKa = 6.71 LFRR397 pKa = 11.84 VEE399 pKa = 3.76 GDD401 pKa = 3.82 GISVRR406 pKa = 11.84 LCSFNGLL413 pKa = 3.61
Molecular weight: 46.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.211
IPC2_protein 9.414
IPC_protein 9.545
Toseland 10.277
ProMoST 9.882
Dawson 10.409
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.833
Grimsley 10.467
Solomon 10.452
Lehninger 10.423
Nozaki 10.262
DTASelect 10.058
Thurlkill 10.292
EMBOSS 10.657
Sillero 10.335
Patrickios 10.482
IPC_peptide 10.452
IPC2_peptide 8.726
IPC2.peptide.svr19 8.519
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2268
85
559
324.0
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.393 ± 0.876
2.381 ± 0.777
6.481 ± 0.642
5.159 ± 0.367
4.145 ± 0.594
6.481 ± 0.57
2.072 ± 0.295
4.409 ± 0.42
4.718 ± 0.677
8.069 ± 0.737
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.94 ± 0.246
3.483 ± 0.766
8.069 ± 1.74
4.056 ± 0.679
5.908 ± 0.643
8.951 ± 0.777
6.834 ± 0.768
6.217 ± 0.514
1.455 ± 0.246
2.778 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here