Ornithinibacillus halophilus
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3462 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5FLS8|A0A1M5FLS8_9BACI Probable molybdenum cofactor guanylyltransferase OS=Ornithinibacillus halophilus OX=930117 GN=mobA PE=3 SV=1
MM1 pKa = 7.46 ALIGLVLILAACGGDD16 pKa = 3.26 KK17 pKa = 11.23 ANIAVGPAGSATNTVSSLILEE38 pKa = 4.48 AYY40 pKa = 9.9 GIEE43 pKa = 4.22 EE44 pKa = 4.02 GDD46 pKa = 3.44 YY47 pKa = 9.91 TAFEE51 pKa = 4.46 EE52 pKa = 5.22 GFGDD56 pKa = 4.68 AADD59 pKa = 4.27 GVQDD63 pKa = 3.75 GNIDD67 pKa = 3.25 ISIGILGLPAGSIEE81 pKa = 4.2 NLHH84 pKa = 6.78 ASAGDD89 pKa = 3.39 AKK91 pKa = 10.24 MLSLSKK97 pKa = 10.5 EE98 pKa = 3.52 AVAYY102 pKa = 9.91 IEE104 pKa = 4.63 EE105 pKa = 4.07 NSAYY109 pKa = 10.34 RR110 pKa = 11.84 EE111 pKa = 3.97 MVIPADD117 pKa = 3.31 SYY119 pKa = 11.46 EE120 pKa = 3.98 FLEE123 pKa = 4.42 EE124 pKa = 4.6 DD125 pKa = 3.77 VTTVTAYY132 pKa = 10.48 AILMGNTNTISEE144 pKa = 4.29 EE145 pKa = 4.02 LGYY148 pKa = 10.79 QLAKK152 pKa = 11.08 VMIEE156 pKa = 3.82 NSSDD160 pKa = 2.86 ISHH163 pKa = 6.63 AQGAQMTLDD172 pKa = 3.31 NALNGLEE179 pKa = 5.04 DD180 pKa = 3.87 MPIHH184 pKa = 6.75 PGAARR189 pKa = 11.84 YY190 pKa = 9.54 YY191 pKa = 9.51 EE192 pKa = 4.13 EE193 pKa = 5.05 QGLEE197 pKa = 3.84 FDD199 pKa = 4.75 NPIAEE204 pKa = 4.47 LNVTEE209 pKa = 5.47 DD210 pKa = 3.5 VTEE213 pKa = 4.16 LTLGTGSSGGTYY225 pKa = 10.06 FPLGGEE231 pKa = 4.29 MATIWKK237 pKa = 10.3 DD238 pKa = 3.36 NIEE241 pKa = 4.74 GINVTSQEE249 pKa = 4.01 TGASLEE255 pKa = 3.84 NMARR259 pKa = 11.84 IGEE262 pKa = 4.27 GTMDD266 pKa = 4.28 LGMAVHH272 pKa = 6.94 GPAMDD277 pKa = 3.98 GVNGEE282 pKa = 4.74 GEE284 pKa = 3.86 FDD286 pKa = 3.88 APIEE290 pKa = 3.93 NAAFIGHH297 pKa = 7.62 IYY299 pKa = 9.97 PEE301 pKa = 4.28 VVQIVTRR308 pKa = 11.84 EE309 pKa = 4.02 STDD312 pKa = 2.82 IDD314 pKa = 3.66 SFEE317 pKa = 4.37 DD318 pKa = 3.44 LKK320 pKa = 11.61
Molecular weight: 33.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.77
IPC_protein 3.745
Toseland 3.554
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.579
Grimsley 3.465
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.592
EMBOSS 3.617
Sillero 3.859
Patrickios 1.176
IPC_peptide 3.706
IPC2_peptide 3.846
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A1M5ME28|A0A1M5ME28_9BACI Ribosomal RNA small subunit methyltransferase A OS=Ornithinibacillus halophilus OX=930117 GN=rsmA PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.22 KK14 pKa = 8.69 VHH16 pKa = 5.46 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.49 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.749
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.486
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3462
0
3462
975949
25
3510
281.9
31.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.292 ± 0.049
0.587 ± 0.011
5.529 ± 0.038
7.938 ± 0.048
4.544 ± 0.034
6.641 ± 0.047
2.04 ± 0.021
8.283 ± 0.042
6.793 ± 0.045
9.517 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.736 ± 0.021
4.915 ± 0.036
3.508 ± 0.025
3.704 ± 0.033
3.822 ± 0.032
5.937 ± 0.032
5.451 ± 0.03
7.081 ± 0.038
1.001 ± 0.016
3.684 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here