Bathymodiolus azoricus thioautotrophic gill symbiont

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; sulfur-oxidizing symbionts

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6K169|A0A1H6K169_9GAMM [similarity to] MloA protein OS=Bathymodiolus azoricus thioautotrophic gill symbiont OX=235205 GN=BAZSYMA_ACONTIG104430_1 PE=4 SV=1
MM1 pKa = 8.21PYY3 pKa = 9.21TGIVIMCIGLDD14 pKa = 3.58LTGVPSGDD22 pKa = 3.39SLLILSRR29 pKa = 11.84DD30 pKa = 3.44TLLALMNSAALSS42 pKa = 3.64

Molecular weight: 4.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6KXW9|A0A1H6KXW9_9GAMM 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Bathymodiolus azoricus thioautotrophic gill symbiont OX=235205 GN=fabZ PE=3 SV=1
MM1 pKa = 7.82PSLTVTVIVISPIAVMRR18 pKa = 11.84RR19 pKa = 11.84LVLSWLRR26 pKa = 11.84LTISKK31 pKa = 10.27SALLVMVKK39 pKa = 9.25VRR41 pKa = 11.84ASAAVSISVALRR53 pKa = 11.84IPTTVPMGG61 pKa = 3.54

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2029

0

2029

479403

34

1773

236.3

26.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.282 ± 0.058

1.1 ± 0.026

5.574 ± 0.051

5.622 ± 0.065

4.539 ± 0.049

6.425 ± 0.062

2.115 ± 0.031

7.95 ± 0.048

7.398 ± 0.059

10.015 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.036

5.325 ± 0.049

3.384 ± 0.036

4.123 ± 0.045

3.676 ± 0.038

6.708 ± 0.057

5.405 ± 0.043

6.541 ± 0.054

1.002 ± 0.021

3.203 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski