Bacillus phage Stitch

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Northropvirinae; Claudivirus; Bacillus virus Stitch

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1PAL9|A0A1B1PAL9_9CAUD Uncharacterized protein OS=Bacillus phage Stitch OX=1874002 GN=STITCH_2 PE=4 SV=1
MM1 pKa = 7.74ALQTITLDD9 pKa = 3.59FTGKK13 pKa = 9.92IAGSTTQNPNIAYY26 pKa = 9.95FNDD29 pKa = 3.43VEE31 pKa = 4.58FTQLQYY37 pKa = 11.44DD38 pKa = 3.72QIFGSEE44 pKa = 4.13LRR46 pKa = 11.84VATTDD51 pKa = 3.23VLKK54 pKa = 11.09FEE56 pKa = 4.87FPVPTEE62 pKa = 3.8AQINLQSVGIYY73 pKa = 9.97YY74 pKa = 10.4KK75 pKa = 10.76SKK77 pKa = 10.67LDD79 pKa = 3.44SGEE82 pKa = 4.08NEE84 pKa = 4.25LRR86 pKa = 11.84SSDD89 pKa = 3.28KK90 pKa = 11.04GVYY93 pKa = 10.21NDD95 pKa = 3.84DD96 pKa = 3.07TTMYY100 pKa = 9.62KK101 pKa = 10.16QKK103 pKa = 10.61LLEE106 pKa = 4.4IYY108 pKa = 10.65GIDD111 pKa = 4.54IQDD114 pKa = 3.25QTIKK118 pKa = 10.89FNITTTGDD126 pKa = 3.36YY127 pKa = 10.86GVYY130 pKa = 9.45EE131 pKa = 4.48VKK133 pKa = 10.76CDD135 pKa = 3.52VTYY138 pKa = 11.34DD139 pKa = 3.67DD140 pKa = 5.31AGLNIYY146 pKa = 9.46GGQYY150 pKa = 9.94QYY152 pKa = 11.49SDD154 pKa = 3.1NGGVVVTVPIQNKK167 pKa = 9.11KK168 pKa = 10.52LEE170 pKa = 4.68EE171 pKa = 3.95ITTEE175 pKa = 4.16LEE177 pKa = 3.9GRR179 pKa = 11.84PVNSVTNVTFAQVGTEE195 pKa = 3.92LVPP198 pKa = 4.06

Molecular weight: 22.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1PAM2|A0A1B1PAM2_9CAUD Uncharacterized protein OS=Bacillus phage Stitch OX=1874002 GN=STITCH_12 PE=4 SV=1
MM1 pKa = 7.27GVAIDD6 pKa = 3.88MTGKK10 pKa = 10.27KK11 pKa = 9.85YY12 pKa = 10.95NRR14 pKa = 11.84LTCIEE19 pKa = 4.5KK20 pKa = 10.7LEE22 pKa = 3.97QRR24 pKa = 11.84QKK26 pKa = 10.89GHH28 pKa = 5.61IMWRR32 pKa = 11.84FKK34 pKa = 10.77CDD36 pKa = 3.1CGKK39 pKa = 10.97VITARR44 pKa = 11.84GGDD47 pKa = 3.5VRR49 pKa = 11.84QGKK52 pKa = 6.87TVSCGCRR59 pKa = 11.84TIEE62 pKa = 3.88IGKK65 pKa = 9.21NNRR68 pKa = 11.84GKK70 pKa = 10.89SNEE73 pKa = 3.71EE74 pKa = 3.54RR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 10.42KK78 pKa = 10.68SLSKK82 pKa = 10.97

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

6834

27

717

189.8

21.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.522 ± 0.385

1.054 ± 0.171

6.731 ± 0.263

7.887 ± 0.573

4.756 ± 0.309

6.57 ± 0.48

1.419 ± 0.17

6.848 ± 0.344

9.716 ± 0.591

6.687 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.336 ± 0.261

6.921 ± 0.376

2.458 ± 0.243

3.439 ± 0.301

3.98 ± 0.334

5.282 ± 0.261

6.014 ± 0.547

6.394 ± 0.416

1.215 ± 0.164

4.77 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski