Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; Sphingomonas wittichii

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5313 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5V4X8|A5V4X8_SPHWW Catechol 1 2-dioxygenase OS=Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) OX=392499 GN=Swit_0977 PE=3 SV=1
MM1 pKa = 7.9TIRR4 pKa = 11.84LCATDD9 pKa = 5.74DD10 pKa = 3.56IPEE13 pKa = 4.39GEE15 pKa = 4.32VRR17 pKa = 11.84QFAVDD22 pKa = 3.63DD23 pKa = 4.15GRR25 pKa = 11.84TLAVYY30 pKa = 10.0RR31 pKa = 11.84VDD33 pKa = 3.25GDD35 pKa = 3.91YY36 pKa = 10.93FATDD40 pKa = 3.97DD41 pKa = 4.3LCTHH45 pKa = 6.85GDD47 pKa = 3.27ASLSEE52 pKa = 4.4GEE54 pKa = 4.39IEE56 pKa = 5.33DD57 pKa = 4.63GKK59 pKa = 10.77ILCPYY64 pKa = 10.28HH65 pKa = 6.5MGSFDD70 pKa = 3.12IRR72 pKa = 11.84TGEE75 pKa = 3.88ACAAPCSIAIKK86 pKa = 9.01TYY88 pKa = 10.44RR89 pKa = 11.84LAVDD93 pKa = 3.86GDD95 pKa = 4.23DD96 pKa = 5.25VLLLDD101 pKa = 4.44

Molecular weight: 10.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5VCJ4|A5VCJ4_SPHWW 2-octaprenyl-3-methyl-6-methoxy-1 4-benzoquinol hydroxylase OS=Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) OX=392499 GN=Swit_3664 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5313

0

5313

1783296

33

1934

335.6

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.585 ± 0.05

0.81 ± 0.01

6.185 ± 0.025

5.259 ± 0.032

3.559 ± 0.019

9.17 ± 0.037

2.074 ± 0.016

5.059 ± 0.021

2.661 ± 0.024

9.813 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.015

2.339 ± 0.023

5.53 ± 0.025

2.9 ± 0.018

7.92 ± 0.034

5.08 ± 0.026

4.962 ± 0.023

7.05 ± 0.025

1.39 ± 0.013

2.283 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski