Lysobacter arseniciresistens ZS79

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; Lysobacter arseniciresistens

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2363 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A0F054|A0A0A0F054_9GAMM Type IV secretion protein Rhs OS=Lysobacter arseniciresistens ZS79 OX=913325 GN=N799_03160 PE=4 SV=1
MM1 pKa = 7.45AASSNTKK8 pKa = 8.62TLLMTLTAALALSACGGGSDD28 pKa = 4.04NVASPGEE35 pKa = 4.41GAFPPPPSSTPPPTTPPPTTPPPTTPPPTAAEE67 pKa = 4.55CPTGTTDD74 pKa = 2.97VGEE77 pKa = 4.39VAGLTNCQLPSRR89 pKa = 11.84IIGEE93 pKa = 4.21LLLPQVDD100 pKa = 3.75GLVYY104 pKa = 10.48SVNGRR109 pKa = 11.84TDD111 pKa = 2.77VGEE114 pKa = 4.42DD115 pKa = 3.28MGGDD119 pKa = 3.58AANPAPQARR128 pKa = 11.84KK129 pKa = 7.67GTLRR133 pKa = 11.84IEE135 pKa = 4.44PGVTVFGSAGADD147 pKa = 2.93FLMVNRR153 pKa = 11.84GSQIFAEE160 pKa = 4.63GDD162 pKa = 3.16AANPIVFTSRR172 pKa = 11.84QSVEE176 pKa = 3.61GTTDD180 pKa = 2.9VDD182 pKa = 6.05SIGQWGGLVLLGRR195 pKa = 11.84AAISTCPGTAQPGTATCEE213 pKa = 4.19AQVEE217 pKa = 4.64GANGYY222 pKa = 10.25YY223 pKa = 10.31GGNANDD229 pKa = 4.87DD230 pKa = 3.58NTGVLKK236 pKa = 9.94YY237 pKa = 10.58VRR239 pKa = 11.84VMHH242 pKa = 6.56SGFEE246 pKa = 3.99VLPDD250 pKa = 3.47VEE252 pKa = 5.22LNGITLAGLGSGTTVEE268 pKa = 4.4YY269 pKa = 10.87VQVHH273 pKa = 5.81NSSDD277 pKa = 4.31DD278 pKa = 3.51GFEE281 pKa = 4.14WFGGTVNAKK290 pKa = 10.1YY291 pKa = 10.52LVGTGNDD298 pKa = 4.0DD299 pKa = 3.62DD300 pKa = 5.28TFDD303 pKa = 3.68TDD305 pKa = 2.65SGYY308 pKa = 10.87RR309 pKa = 11.84GGIQFGLILQRR320 pKa = 11.84AGGGDD325 pKa = 3.26RR326 pKa = 11.84MNEE329 pKa = 3.99MSSQDD334 pKa = 3.32LPYY337 pKa = 10.91RR338 pKa = 11.84SMPNLANVTFVGAGHH353 pKa = 7.4DD354 pKa = 3.63NAIVLNQGTLVGYY367 pKa = 9.73YY368 pKa = 9.89NAVVTGGAPACVEE381 pKa = 4.27FQSDD385 pKa = 3.46TTDD388 pKa = 3.29GVFHH392 pKa = 6.22STYY395 pKa = 10.29LSCDD399 pKa = 3.17VPFAGGASGRR409 pKa = 11.84EE410 pKa = 3.53AAAFNAGTNNATGASTLTGGFINGANEE437 pKa = 3.98AAVPAYY443 pKa = 10.34AGLASVNAAFADD455 pKa = 3.34VDD457 pKa = 4.06YY458 pKa = 11.32IGAVKK463 pKa = 10.53DD464 pKa = 5.27AGDD467 pKa = 3.68TWWQGWTCGLTADD480 pKa = 4.67DD481 pKa = 4.6CC482 pKa = 5.0

Molecular weight:
48.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A0EWZ7|A0A0A0EWZ7_9GAMM TonB-dependent receptor OS=Lysobacter arseniciresistens ZS79 OX=913325 GN=N799_08535 PE=3 SV=1
MM1 pKa = 7.14QSKK4 pKa = 10.73LGLRR8 pKa = 11.84KK9 pKa = 9.51SKK11 pKa = 10.3RR12 pKa = 11.84VQLSQLCRR20 pKa = 11.84WRR22 pKa = 11.84TMSMRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 7.98LTKK32 pKa = 10.35RR33 pKa = 11.84LTKK36 pKa = 10.19QSMRR40 pKa = 11.84RR41 pKa = 11.84SIKK44 pKa = 8.86QSRR47 pKa = 11.84SSRR50 pKa = 11.84FQLPTTKK57 pKa = 10.41AWMQQ61 pKa = 3.24

Molecular weight:
7.46 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2363

0

2363

756334

41

1827

320.1

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.355 ± 0.075

0.773 ± 0.018

6.337 ± 0.047

5.692 ± 0.05

3.307 ± 0.029

8.887 ± 0.045

2.28 ± 0.025

3.98 ± 0.038

2.525 ± 0.044

10.654 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.025

2.312 ± 0.03

5.34 ± 0.04

3.365 ± 0.031

7.922 ± 0.058

4.722 ± 0.038

4.859 ± 0.039

7.819 ± 0.048

1.451 ± 0.022

2.197 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski