Lysobacter arseniciresistens ZS79
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2363 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0F054|A0A0A0F054_9GAMM Type IV secretion protein Rhs OS=Lysobacter arseniciresistens ZS79 OX=913325 GN=N799_03160 PE=4 SV=1
MM1 pKa = 7.45 AASSNTKK8 pKa = 8.62 TLLMTLTAALALSACGGGSDD28 pKa = 4.04 NVASPGEE35 pKa = 4.41 GAFPPPPSSTPPPTTPPPTTPPPTTPPPTAAEE67 pKa = 4.55 CPTGTTDD74 pKa = 2.97 VGEE77 pKa = 4.39 VAGLTNCQLPSRR89 pKa = 11.84 IIGEE93 pKa = 4.21 LLLPQVDD100 pKa = 3.75 GLVYY104 pKa = 10.48 SVNGRR109 pKa = 11.84 TDD111 pKa = 2.77 VGEE114 pKa = 4.42 DD115 pKa = 3.28 MGGDD119 pKa = 3.58 AANPAPQARR128 pKa = 11.84 KK129 pKa = 7.67 GTLRR133 pKa = 11.84 IEE135 pKa = 4.44 PGVTVFGSAGADD147 pKa = 2.93 FLMVNRR153 pKa = 11.84 GSQIFAEE160 pKa = 4.63 GDD162 pKa = 3.16 AANPIVFTSRR172 pKa = 11.84 QSVEE176 pKa = 3.61 GTTDD180 pKa = 2.9 VDD182 pKa = 6.05 SIGQWGGLVLLGRR195 pKa = 11.84 AAISTCPGTAQPGTATCEE213 pKa = 4.19 AQVEE217 pKa = 4.64 GANGYY222 pKa = 10.25 YY223 pKa = 10.31 GGNANDD229 pKa = 4.87 DD230 pKa = 3.58 NTGVLKK236 pKa = 9.94 YY237 pKa = 10.58 VRR239 pKa = 11.84 VMHH242 pKa = 6.56 SGFEE246 pKa = 3.99 VLPDD250 pKa = 3.47 VEE252 pKa = 5.22 LNGITLAGLGSGTTVEE268 pKa = 4.4 YY269 pKa = 10.87 VQVHH273 pKa = 5.81 NSSDD277 pKa = 4.31 DD278 pKa = 3.51 GFEE281 pKa = 4.14 WFGGTVNAKK290 pKa = 10.1 YY291 pKa = 10.52 LVGTGNDD298 pKa = 4.0 DD299 pKa = 3.62 DD300 pKa = 5.28 TFDD303 pKa = 3.68 TDD305 pKa = 2.65 SGYY308 pKa = 10.87 RR309 pKa = 11.84 GGIQFGLILQRR320 pKa = 11.84 AGGGDD325 pKa = 3.26 RR326 pKa = 11.84 MNEE329 pKa = 3.99 MSSQDD334 pKa = 3.32 LPYY337 pKa = 10.91 RR338 pKa = 11.84 SMPNLANVTFVGAGHH353 pKa = 7.4 DD354 pKa = 3.63 NAIVLNQGTLVGYY367 pKa = 9.73 YY368 pKa = 9.89 NAVVTGGAPACVEE381 pKa = 4.27 FQSDD385 pKa = 3.46 TTDD388 pKa = 3.29 GVFHH392 pKa = 6.22 STYY395 pKa = 10.29 LSCDD399 pKa = 3.17 VPFAGGASGRR409 pKa = 11.84 EE410 pKa = 3.53 AAAFNAGTNNATGASTLTGGFINGANEE437 pKa = 3.98 AAVPAYY443 pKa = 10.34 AGLASVNAAFADD455 pKa = 3.34 VDD457 pKa = 4.06 YY458 pKa = 11.32 IGAVKK463 pKa = 10.53 DD464 pKa = 5.27 AGDD467 pKa = 3.68 TWWQGWTCGLTADD480 pKa = 4.67 DD481 pKa = 4.6 CC482 pKa = 5.0
Molecular weight: 48.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.935
IPC_protein 3.948
Toseland 3.732
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.77
Grimsley 3.63
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.291
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.062
Patrickios 0.884
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.962
Protein with the highest isoelectric point:
>tr|A0A0A0EWZ7|A0A0A0EWZ7_9GAMM TonB-dependent receptor OS=Lysobacter arseniciresistens ZS79 OX=913325 GN=N799_08535 PE=3 SV=1
MM1 pKa = 7.14 QSKK4 pKa = 10.73 LGLRR8 pKa = 11.84 KK9 pKa = 9.51 SKK11 pKa = 10.3 RR12 pKa = 11.84 VQLSQLCRR20 pKa = 11.84 WRR22 pKa = 11.84 TMSMRR27 pKa = 11.84 RR28 pKa = 11.84 KK29 pKa = 7.98 LTKK32 pKa = 10.35 RR33 pKa = 11.84 LTKK36 pKa = 10.19 QSMRR40 pKa = 11.84 RR41 pKa = 11.84 SIKK44 pKa = 8.86 QSRR47 pKa = 11.84 SSRR50 pKa = 11.84 FQLPTTKK57 pKa = 10.41 AWMQQ61 pKa = 3.24
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.427
IPC2_protein 10.921
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2363
0
2363
756334
41
1827
320.1
34.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.355 ± 0.075
0.773 ± 0.018
6.337 ± 0.047
5.692 ± 0.05
3.307 ± 0.029
8.887 ± 0.045
2.28 ± 0.025
3.98 ± 0.038
2.525 ± 0.044
10.654 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.224 ± 0.025
2.312 ± 0.03
5.34 ± 0.04
3.365 ± 0.031
7.922 ± 0.058
4.722 ± 0.038
4.859 ± 0.039
7.819 ± 0.048
1.451 ± 0.022
2.197 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here