Canis familiaris papillomavirus 8
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3DRD9|G3DRD9_9PAPI Replication protein E1 OS=Canis familiaris papillomavirus 8 OX=1081055 GN=E1 PE=3 SV=1
MM1 pKa = 7.39 IGKK4 pKa = 9.27 DD5 pKa = 2.95 ATLRR9 pKa = 11.84 DD10 pKa = 3.45 IVLEE14 pKa = 4.07 EE15 pKa = 4.11 EE16 pKa = 4.49 QPCPVDD22 pKa = 5.48 DD23 pKa = 5.15 LYY25 pKa = 11.53 CDD27 pKa = 3.72 EE28 pKa = 4.87 EE29 pKa = 4.51 LPPEE33 pKa = 4.26 EE34 pKa = 4.28 EE35 pKa = 4.15 EE36 pKa = 3.89 EE37 pKa = 4.6 AEE39 pKa = 4.98 RR40 pKa = 11.84 EE41 pKa = 4.27 LQSFAPYY48 pKa = 10.0 RR49 pKa = 11.84 IQAHH53 pKa = 6.52 CGHH56 pKa = 7.06 CEE58 pKa = 3.49 RR59 pKa = 11.84 PIRR62 pKa = 11.84 LVVLSTRR69 pKa = 11.84 DD70 pKa = 3.73 GIISLEE76 pKa = 4.0 EE77 pKa = 4.18 LLTSCLDD84 pKa = 3.92 LCCPDD89 pKa = 3.48 CASRR93 pKa = 11.84 RR94 pKa = 11.84 WRR96 pKa = 11.84 FGRR99 pKa = 11.84 AQHH102 pKa = 6.55 GGG104 pKa = 3.34
Molecular weight: 11.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.383
IPC2_protein 4.533
IPC_protein 4.418
Toseland 4.279
ProMoST 4.52
Dawson 4.368
Bjellqvist 4.507
Wikipedia 4.215
Rodwell 4.266
Grimsley 4.19
Solomon 4.355
Lehninger 4.317
Nozaki 4.469
DTASelect 4.596
Thurlkill 4.279
EMBOSS 4.24
Sillero 4.533
Patrickios 3.16
IPC_peptide 4.368
IPC2_peptide 4.533
IPC2.peptide.svr19 4.455
Protein with the highest isoelectric point:
>tr|G3DRE2|G3DRE2_9PAPI Minor capsid protein L2 OS=Canis familiaris papillomavirus 8 OX=1081055 GN=L2 PE=3 SV=1
MM1 pKa = 7.02 QRR3 pKa = 11.84 DD4 pKa = 3.48 IVLRR8 pKa = 11.84 EE9 pKa = 3.74 YY10 pKa = 11.39 GRR12 pKa = 11.84 LCITMRR18 pKa = 11.84 PFHH21 pKa = 6.6 LAILLLALHH30 pKa = 7.03 RR31 pKa = 11.84 PPTLVEE37 pKa = 3.77 RR38 pKa = 11.84 LNYY41 pKa = 9.79 ILTGYY46 pKa = 8.52 PPPNDD51 pKa = 3.25 EE52 pKa = 4.75 PEE54 pKa = 4.06 TGEE57 pKa = 3.91 RR58 pKa = 11.84 SPARR62 pKa = 11.84 GRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 RR67 pKa = 11.84 RR68 pKa = 11.84 KK69 pKa = 9.1 EE70 pKa = 3.77 RR71 pKa = 11.84 LSSPQFTPPHH81 pKa = 5.73 TPPRR85 pKa = 11.84 SPTALLTPPSPFPSLLGGFSDD106 pKa = 4.56 PPSSQWEE113 pKa = 4.19 LPPSQRR119 pKa = 11.84 RR120 pKa = 11.84 LAQGRR125 pKa = 11.84 SRR127 pKa = 11.84 GPTPPKK133 pKa = 9.97 HH134 pKa = 6.5 PEE136 pKa = 3.9 SQRR139 pKa = 11.84 SDD141 pKa = 3.58 SPHH144 pKa = 7.14 PPQQGPGPSASQRR157 pKa = 11.84 IVSLPRR163 pKa = 11.84 GVPRR167 pKa = 11.84 SPRR170 pKa = 11.84 TNPFWFPFTEE180 pKa = 4.41 SVEE183 pKa = 4.23 EE184 pKa = 4.17 EE185 pKa = 4.44 GTGLSVVRR193 pKa = 11.84 GGGNNTPGPLRR204 pKa = 11.84 SRR206 pKa = 11.84 PQTPDD211 pKa = 2.13 HH212 pKa = 7.22 WDD214 pKa = 3.45 PLPRR218 pKa = 11.84 PEE220 pKa = 4.04 EE221 pKa = 3.92 VPRR224 pKa = 11.84 RR225 pKa = 11.84 RR226 pKa = 11.84 RR227 pKa = 11.84 RR228 pKa = 11.84 PRR230 pKa = 11.84 LQQQHH235 pKa = 5.92 EE236 pKa = 4.39 DD237 pKa = 3.45 LRR239 pKa = 11.84 LALQQLGRR247 pKa = 11.84 DD248 pKa = 3.55 AFQLRR253 pKa = 11.84 EE254 pKa = 4.56 DD255 pKa = 4.42 IEE257 pKa = 4.45 TDD259 pKa = 3.22 LNTFFGKK266 pKa = 10.57 LGIAPHH272 pKa = 6.65 RR273 pKa = 11.84 LL274 pKa = 3.19
Molecular weight: 31.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.179
IPC2_protein 9.75
IPC_protein 11.199
Toseland 11.316
ProMoST 11.784
Dawson 11.316
Bjellqvist 11.301
Wikipedia 11.784
Rodwell 10.965
Grimsley 11.359
Solomon 11.784
Lehninger 11.696
Nozaki 11.301
DTASelect 11.301
Thurlkill 11.316
EMBOSS 11.798
Sillero 11.316
Patrickios 10.701
IPC_peptide 11.798
IPC2_peptide 10.774
IPC2.peptide.svr19 9.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2680
104
625
382.9
42.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.53 ± 0.732
2.127 ± 0.569
6.194 ± 0.597
6.194 ± 0.553
3.993 ± 0.781
7.91 ± 0.503
2.313 ± 0.31
3.694 ± 0.57
3.843 ± 0.835
8.284 ± 0.86
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.642 ± 0.406
3.209 ± 0.751
8.582 ± 1.532
4.254 ± 0.529
7.612 ± 0.918
7.575 ± 0.487
6.269 ± 0.406
6.007 ± 1.019
1.567 ± 0.287
2.201 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here