Amino acid dipepetide frequency for Pseudomonas phage vB_PsyM_KIL1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.356AlaAla: 7.356 ± 0.793
0.983AlaCys: 0.983 ± 0.215
4.878AlaAsp: 4.878 ± 0.487
4.72AlaGlu: 4.72 ± 0.449
3.226AlaPhe: 3.226 ± 0.334
5.035AlaGly: 5.035 ± 0.401
1.377AlaHis: 1.377 ± 0.235
4.72AlaIle: 4.72 ± 0.391
4.799AlaLys: 4.799 ± 0.413
6.333AlaLeu: 6.333 ± 0.586
2.163AlaMet: 2.163 ± 0.313
3.147AlaAsn: 3.147 ± 0.43
2.321AlaPro: 2.321 ± 0.316
3.58AlaGln: 3.58 ± 0.352
4.052AlaArg: 4.052 ± 0.502
4.76AlaSer: 4.76 ± 0.497
5.153AlaThr: 5.153 ± 0.573
5.153AlaVal: 5.153 ± 0.519
1.337AlaTrp: 1.337 ± 0.251
2.203AlaTyr: 2.203 ± 0.258
0.0AlaXaa: 0.0 ± 0.0
Cys
0.944CysAla: 0.944 ± 0.194
0.236CysCys: 0.236 ± 0.105
0.629CysAsp: 0.629 ± 0.176
0.59CysGlu: 0.59 ± 0.196
0.511CysPhe: 0.511 ± 0.158
1.062CysGly: 1.062 ± 0.244
0.393CysHis: 0.393 ± 0.116
0.747CysIle: 0.747 ± 0.164
1.101CysLys: 1.101 ± 0.227
1.023CysLeu: 1.023 ± 0.199
0.354CysMet: 0.354 ± 0.124
0.669CysAsn: 0.669 ± 0.174
0.708CysPro: 0.708 ± 0.158
0.472CysGln: 0.472 ± 0.13
0.669CysArg: 0.669 ± 0.16
1.141CysSer: 1.141 ± 0.22
0.905CysThr: 0.905 ± 0.198
1.062CysVal: 1.062 ± 0.191
0.157CysTrp: 0.157 ± 0.074
0.708CysTyr: 0.708 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
4.012AspAla: 4.012 ± 0.359
0.983AspCys: 0.983 ± 0.192
3.383AspAsp: 3.383 ± 0.374
3.658AspGlu: 3.658 ± 0.442
2.478AspPhe: 2.478 ± 0.316
4.799AspGly: 4.799 ± 0.453
0.983AspHis: 0.983 ± 0.197
2.675AspIle: 2.675 ± 0.331
4.72AspLys: 4.72 ± 0.471
5.507AspLeu: 5.507 ± 0.493
2.085AspMet: 2.085 ± 0.305
2.478AspAsn: 2.478 ± 0.287
1.927AspPro: 1.927 ± 0.275
1.888AspGln: 1.888 ± 0.28
2.557AspArg: 2.557 ± 0.313
3.304AspSer: 3.304 ± 0.384
3.344AspThr: 3.344 ± 0.391
3.816AspVal: 3.816 ± 0.374
1.573AspTrp: 1.573 ± 0.226
2.478AspTyr: 2.478 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
5.822GluAla: 5.822 ± 0.617
1.18GluCys: 1.18 ± 0.204
3.776GluAsp: 3.776 ± 0.405
5.822GluGlu: 5.822 ± 0.61
3.462GluPhe: 3.462 ± 0.418
3.383GluGly: 3.383 ± 0.398
1.298GluHis: 1.298 ± 0.25
3.934GluIle: 3.934 ± 0.432
3.501GluLys: 3.501 ± 0.381
5.586GluLeu: 5.586 ± 0.527
2.399GluMet: 2.399 ± 0.299
2.399GluAsn: 2.399 ± 0.31
1.77GluPro: 1.77 ± 0.272
2.636GluGln: 2.636 ± 0.338
3.698GluArg: 3.698 ± 0.434
4.091GluSer: 4.091 ± 0.397
3.265GluThr: 3.265 ± 0.333
4.76GluVal: 4.76 ± 0.38
1.534GluTrp: 1.534 ± 0.258
2.636GluTyr: 2.636 ± 0.284
0.0GluXaa: 0.0 ± 0.0
Phe
2.36PheAla: 2.36 ± 0.383
0.708PheCys: 0.708 ± 0.163
2.518PheAsp: 2.518 ± 0.344
3.304PheGlu: 3.304 ± 0.364
1.259PhePhe: 1.259 ± 0.249
3.108PheGly: 3.108 ± 0.384
0.787PheHis: 0.787 ± 0.175
2.006PheIle: 2.006 ± 0.335
2.557PheLys: 2.557 ± 0.327
3.068PheLeu: 3.068 ± 0.358
1.101PheMet: 1.101 ± 0.179
2.045PheAsn: 2.045 ± 0.255
1.455PhePro: 1.455 ± 0.257
1.377PheGln: 1.377 ± 0.201
1.77PheArg: 1.77 ± 0.288
3.304PheSer: 3.304 ± 0.34
2.832PheThr: 2.832 ± 0.329
2.557PheVal: 2.557 ± 0.3
0.433PheTrp: 0.433 ± 0.167
0.944PheTyr: 0.944 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
4.248GlyAla: 4.248 ± 0.495
1.377GlyCys: 1.377 ± 0.238
3.973GlyAsp: 3.973 ± 0.445
3.894GlyGlu: 3.894 ± 0.345
3.108GlyPhe: 3.108 ± 0.343
5.468GlyGly: 5.468 ± 0.588
1.377GlyHis: 1.377 ± 0.284
3.816GlyIle: 3.816 ± 0.459
5.822GlyLys: 5.822 ± 0.522
6.687GlyLeu: 6.687 ± 0.682
2.006GlyMet: 2.006 ± 0.272
3.462GlyAsn: 3.462 ± 0.498
1.18GlyPro: 1.18 ± 0.198
1.888GlyGln: 1.888 ± 0.273
3.54GlyArg: 3.54 ± 0.366
5.271GlySer: 5.271 ± 0.546
3.855GlyThr: 3.855 ± 0.507
6.176GlyVal: 6.176 ± 0.445
1.455GlyTrp: 1.455 ± 0.252
3.658GlyTyr: 3.658 ± 0.393
0.0GlyXaa: 0.0 ± 0.0
His
1.141HisAla: 1.141 ± 0.217
0.315HisCys: 0.315 ± 0.091
0.865HisAsp: 0.865 ± 0.163
1.062HisGlu: 1.062 ± 0.164
0.787HisPhe: 0.787 ± 0.198
1.298HisGly: 1.298 ± 0.202
0.354HisHis: 0.354 ± 0.114
1.023HisIle: 1.023 ± 0.208
1.18HisLys: 1.18 ± 0.216
1.455HisLeu: 1.455 ± 0.25
0.59HisMet: 0.59 ± 0.142
1.101HisAsn: 1.101 ± 0.217
0.826HisPro: 0.826 ± 0.194
0.511HisGln: 0.511 ± 0.154
1.18HisArg: 1.18 ± 0.206
1.534HisSer: 1.534 ± 0.219
1.101HisThr: 1.101 ± 0.191
1.219HisVal: 1.219 ± 0.226
0.393HisTrp: 0.393 ± 0.125
0.669HisTyr: 0.669 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
3.934IleAla: 3.934 ± 0.421
0.826IleCys: 0.826 ± 0.182
3.816IleAsp: 3.816 ± 0.426
4.406IleGlu: 4.406 ± 0.405
1.652IlePhe: 1.652 ± 0.254
3.894IleGly: 3.894 ± 0.445
1.062IleHis: 1.062 ± 0.179
2.636IleIle: 2.636 ± 0.336
3.816IleLys: 3.816 ± 0.407
4.288IleLeu: 4.288 ± 0.5
1.219IleMet: 1.219 ± 0.242
3.068IleAsn: 3.068 ± 0.343
2.439IlePro: 2.439 ± 0.289
2.321IleGln: 2.321 ± 0.274
3.304IleArg: 3.304 ± 0.3
3.698IleSer: 3.698 ± 0.41
4.012IleThr: 4.012 ± 0.352
3.422IleVal: 3.422 ± 0.439
0.669IleTrp: 0.669 ± 0.159
1.298IleTyr: 1.298 ± 0.191
0.0IleXaa: 0.0 ± 0.0
Lys
6.294LysAla: 6.294 ± 0.562
0.59LysCys: 0.59 ± 0.148
4.327LysAsp: 4.327 ± 0.452
4.602LysGlu: 4.602 ± 0.47
2.321LysPhe: 2.321 ± 0.294
4.327LysGly: 4.327 ± 0.45
1.18LysHis: 1.18 ± 0.216
3.973LysIle: 3.973 ± 0.359
3.462LysLys: 3.462 ± 0.413
4.76LysLeu: 4.76 ± 0.462
2.518LysMet: 2.518 ± 0.292
2.911LysAsn: 2.911 ± 0.423
2.754LysPro: 2.754 ± 0.335
2.714LysGln: 2.714 ± 0.365
3.855LysArg: 3.855 ± 0.38
4.091LysSer: 4.091 ± 0.43
3.894LysThr: 3.894 ± 0.409
5.232LysVal: 5.232 ± 0.438
0.865LysTrp: 0.865 ± 0.183
2.518LysTyr: 2.518 ± 0.336
0.0LysXaa: 0.0 ± 0.0
Leu
6.805LeuAla: 6.805 ± 0.517
1.023LeuCys: 1.023 ± 0.215
5.114LeuAsp: 5.114 ± 0.482
6.018LeuGlu: 6.018 ± 0.469
3.265LeuPhe: 3.265 ± 0.396
6.018LeuGly: 6.018 ± 0.647
1.062LeuHis: 1.062 ± 0.225
4.248LeuIle: 4.248 ± 0.467
5.822LeuLys: 5.822 ± 0.521
5.9LeuLeu: 5.9 ± 0.506
2.203LeuMet: 2.203 ± 0.308
4.248LeuAsn: 4.248 ± 0.497
3.816LeuPro: 3.816 ± 0.332
3.816LeuGln: 3.816 ± 0.44
3.855LeuArg: 3.855 ± 0.425
5.31LeuSer: 5.31 ± 0.477
5.035LeuThr: 5.035 ± 0.554
4.602LeuVal: 4.602 ± 0.463
1.337LeuTrp: 1.337 ± 0.218
2.518LeuTyr: 2.518 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
2.636MetAla: 2.636 ± 0.326
0.236MetCys: 0.236 ± 0.112
0.983MetAsp: 0.983 ± 0.215
2.085MetGlu: 2.085 ± 0.319
1.18MetPhe: 1.18 ± 0.286
1.495MetGly: 1.495 ± 0.227
0.708MetHis: 0.708 ± 0.174
1.849MetIle: 1.849 ± 0.28
2.203MetLys: 2.203 ± 0.345
2.045MetLeu: 2.045 ± 0.328
0.551MetMet: 0.551 ± 0.158
1.337MetAsn: 1.337 ± 0.277
0.944MetPro: 0.944 ± 0.211
1.18MetGln: 1.18 ± 0.188
1.062MetArg: 1.062 ± 0.189
2.399MetSer: 2.399 ± 0.242
1.809MetThr: 1.809 ± 0.307
1.809MetVal: 1.809 ± 0.338
0.354MetTrp: 0.354 ± 0.116
1.062MetTyr: 1.062 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
3.108AsnAla: 3.108 ± 0.356
0.669AsnCys: 0.669 ± 0.175
2.793AsnAsp: 2.793 ± 0.325
2.045AsnGlu: 2.045 ± 0.287
1.652AsnPhe: 1.652 ± 0.212
4.72AsnGly: 4.72 ± 0.451
1.18AsnHis: 1.18 ± 0.196
3.226AsnIle: 3.226 ± 0.366
3.147AsnLys: 3.147 ± 0.344
4.248AsnLeu: 4.248 ± 0.649
0.708AsnMet: 0.708 ± 0.141
2.793AsnAsn: 2.793 ± 0.43
2.281AsnPro: 2.281 ± 0.351
1.337AsnGln: 1.337 ± 0.236
2.36AsnArg: 2.36 ± 0.317
3.776AsnSer: 3.776 ± 0.496
2.911AsnThr: 2.911 ± 0.33
2.832AsnVal: 2.832 ± 0.371
0.629AsnTrp: 0.629 ± 0.139
1.652AsnTyr: 1.652 ± 0.227
0.0AsnXaa: 0.0 ± 0.0
Pro
3.068ProAla: 3.068 ± 0.368
0.669ProCys: 0.669 ± 0.175
2.006ProAsp: 2.006 ± 0.298
3.383ProGlu: 3.383 ± 0.346
1.298ProPhe: 1.298 ± 0.229
1.967ProGly: 1.967 ± 0.267
0.787ProHis: 0.787 ± 0.179
1.731ProIle: 1.731 ± 0.273
1.967ProLys: 1.967 ± 0.319
2.636ProLeu: 2.636 ± 0.283
1.18ProMet: 1.18 ± 0.215
1.809ProAsn: 1.809 ± 0.271
1.023ProPro: 1.023 ± 0.225
1.495ProGln: 1.495 ± 0.279
1.298ProArg: 1.298 ± 0.247
2.36ProSer: 2.36 ± 0.277
2.085ProThr: 2.085 ± 0.311
2.399ProVal: 2.399 ± 0.341
0.511ProTrp: 0.511 ± 0.178
1.534ProTyr: 1.534 ± 0.249
0.0ProXaa: 0.0 ± 0.0
Gln
3.658GlnAla: 3.658 ± 0.416
0.275GlnCys: 0.275 ± 0.107
2.399GlnAsp: 2.399 ± 0.323
1.927GlnGlu: 1.927 ± 0.278
1.573GlnPhe: 1.573 ± 0.25
2.95GlnGly: 2.95 ± 0.33
0.669GlnHis: 0.669 ± 0.144
2.439GlnIle: 2.439 ± 0.272
2.281GlnLys: 2.281 ± 0.293
3.54GlnLeu: 3.54 ± 0.35
1.337GlnMet: 1.337 ± 0.209
1.77GlnAsn: 1.77 ± 0.235
0.905GlnPro: 0.905 ± 0.183
1.691GlnGln: 1.691 ± 0.252
1.849GlnArg: 1.849 ± 0.251
2.163GlnSer: 2.163 ± 0.287
1.888GlnThr: 1.888 ± 0.274
2.872GlnVal: 2.872 ± 0.296
0.826GlnTrp: 0.826 ± 0.182
1.455GlnTyr: 1.455 ± 0.27
0.0GlnXaa: 0.0 ± 0.0
Arg
3.304ArgAla: 3.304 ± 0.36
1.062ArgCys: 1.062 ± 0.184
2.399ArgAsp: 2.399 ± 0.352
2.95ArgGlu: 2.95 ± 0.339
1.731ArgPhe: 1.731 ± 0.229
3.265ArgGly: 3.265 ± 0.446
1.062ArgHis: 1.062 ± 0.219
2.911ArgIle: 2.911 ± 0.289
3.658ArgLys: 3.658 ± 0.432
5.035ArgLeu: 5.035 ± 0.481
1.455ArgMet: 1.455 ± 0.253
2.399ArgAsn: 2.399 ± 0.286
1.534ArgPro: 1.534 ± 0.245
2.045ArgGln: 2.045 ± 0.305
2.675ArgArg: 2.675 ± 0.283
2.911ArgSer: 2.911 ± 0.38
2.754ArgThr: 2.754 ± 0.329
3.344ArgVal: 3.344 ± 0.313
1.062ArgTrp: 1.062 ± 0.181
1.613ArgTyr: 1.613 ± 0.247
0.0ArgXaa: 0.0 ± 0.0
Ser
4.17SerAla: 4.17 ± 0.495
0.826SerCys: 0.826 ± 0.277
3.462SerAsp: 3.462 ± 0.374
4.052SerGlu: 4.052 ± 0.41
2.518SerPhe: 2.518 ± 0.305
6.058SerGly: 6.058 ± 0.761
0.983SerHis: 0.983 ± 0.207
3.973SerIle: 3.973 ± 0.446
4.445SerLys: 4.445 ± 0.424
5.507SerLeu: 5.507 ± 0.527
1.927SerMet: 1.927 ± 0.257
3.186SerAsn: 3.186 ± 0.431
2.478SerPro: 2.478 ± 0.331
2.399SerGln: 2.399 ± 0.276
3.619SerArg: 3.619 ± 0.358
4.563SerSer: 4.563 ± 0.566
4.288SerThr: 4.288 ± 0.602
4.76SerVal: 4.76 ± 0.453
1.062SerTrp: 1.062 ± 0.183
2.203SerTyr: 2.203 ± 0.289
0.0SerXaa: 0.0 ± 0.0
Thr
4.406ThrAla: 4.406 ± 0.491
0.59ThrCys: 0.59 ± 0.148
2.872ThrAsp: 2.872 ± 0.388
3.776ThrGlu: 3.776 ± 0.394
2.518ThrPhe: 2.518 ± 0.324
5.389ThrGly: 5.389 ± 0.532
1.023ThrHis: 1.023 ± 0.212
3.934ThrIle: 3.934 ± 0.437
3.973ThrLys: 3.973 ± 0.392
5.153ThrLeu: 5.153 ± 0.326
1.652ThrMet: 1.652 ± 0.233
3.186ThrAsn: 3.186 ± 0.42
2.832ThrPro: 2.832 ± 0.363
2.399ThrGln: 2.399 ± 0.372
2.399ThrArg: 2.399 ± 0.371
4.012ThrSer: 4.012 ± 0.509
3.816ThrThr: 3.816 ± 0.463
4.445ThrVal: 4.445 ± 0.529
0.551ThrTrp: 0.551 ± 0.146
2.124ThrTyr: 2.124 ± 0.265
0.0ThrXaa: 0.0 ± 0.0
Val
6.215ValAla: 6.215 ± 0.616
1.023ValCys: 1.023 ± 0.189
4.72ValAsp: 4.72 ± 0.458
4.681ValGlu: 4.681 ± 0.579
2.911ValPhe: 2.911 ± 0.325
4.602ValGly: 4.602 ± 0.578
1.101ValHis: 1.101 ± 0.249
3.186ValIle: 3.186 ± 0.398
4.799ValLys: 4.799 ± 0.44
4.76ValLeu: 4.76 ± 0.47
1.534ValMet: 1.534 ± 0.289
3.265ValAsn: 3.265 ± 0.382
2.085ValPro: 2.085 ± 0.345
2.675ValGln: 2.675 ± 0.367
3.029ValArg: 3.029 ± 0.362
4.091ValSer: 4.091 ± 0.413
4.878ValThr: 4.878 ± 0.566
4.76ValVal: 4.76 ± 0.561
0.944ValTrp: 0.944 ± 0.205
3.147ValTyr: 3.147 ± 0.324
0.0ValXaa: 0.0 ± 0.0
Trp
1.416TrpAla: 1.416 ± 0.254
0.157TrpCys: 0.157 ± 0.078
1.259TrpAsp: 1.259 ± 0.193
1.062TrpGlu: 1.062 ± 0.243
0.629TrpPhe: 0.629 ± 0.163
0.747TrpGly: 0.747 ± 0.185
0.59TrpHis: 0.59 ± 0.156
0.983TrpIle: 0.983 ± 0.187
1.455TrpLys: 1.455 ± 0.206
2.163TrpLeu: 2.163 ± 0.322
0.275TrpMet: 0.275 ± 0.096
0.551TrpAsn: 0.551 ± 0.126
0.511TrpPro: 0.511 ± 0.157
0.826TrpGln: 0.826 ± 0.198
0.747TrpArg: 0.747 ± 0.158
0.747TrpSer: 0.747 ± 0.141
0.511TrpThr: 0.511 ± 0.132
1.219TrpVal: 1.219 ± 0.238
0.315TrpTrp: 0.315 ± 0.109
0.59TrpTyr: 0.59 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.399TyrAla: 2.399 ± 0.325
0.354TyrCys: 0.354 ± 0.108
2.478TyrAsp: 2.478 ± 0.323
2.911TyrGlu: 2.911 ± 0.33
1.416TyrPhe: 1.416 ± 0.226
2.675TyrGly: 2.675 ± 0.31
0.629TyrHis: 0.629 ± 0.169
1.77TyrIle: 1.77 ± 0.289
2.478TyrLys: 2.478 ± 0.338
2.321TyrLeu: 2.321 ± 0.323
0.629TyrMet: 0.629 ± 0.174
2.242TyrAsn: 2.242 ± 0.316
1.416TyrPro: 1.416 ± 0.211
1.219TyrGln: 1.219 ± 0.207
1.652TyrArg: 1.652 ± 0.219
2.99TyrSer: 2.99 ± 0.482
2.714TyrThr: 2.714 ± 0.334
1.967TyrVal: 1.967 ± 0.271
0.747TyrTrp: 0.747 ± 0.183
1.141TyrTyr: 1.141 ± 0.233
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 159 proteins (25423 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski